Calcium-sensing receptor acts as an antiviral factor for rotavirus infections and participates in cellular antiviral response
Objective(s): Rotavirus (RV) is one of the most significant pathogens associated with childhood diarrhoeal deaths worldwide. Elevated cytoplasmic calcium is required for RV replication, but the underlying mechanisms responsible for calcium influx remain poorly understood. The Calcium-sensing recepto...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2022-08-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_20706_4d3e1b419318a79562e68c18121f6b9d.pdf |
| _version_ | 1811335769098289152 |
|---|---|
| author | Haohai Huang Dan Liao Bin He Yejia Cui Rong Pu Guanghui Zhou |
| author_facet | Haohai Huang Dan Liao Bin He Yejia Cui Rong Pu Guanghui Zhou |
| author_sort | Haohai Huang |
| collection | DOAJ |
| description | Objective(s): Rotavirus (RV) is one of the most significant pathogens associated with childhood diarrhoeal deaths worldwide. Elevated cytoplasmic calcium is required for RV replication, but the underlying mechanisms responsible for calcium influx remain poorly understood. The Calcium-sensing receptor (CaSR) is an important Ca2+ sensor that regulates the transport of Ca2+ into or out of the extracellular space by affecting the status of Ca2+ ion channels on the membrane of cells. Currently, the function of CaSR in RV replication is unclear. Materials and Methods: We evaluated the mRNA and protein levels of CaSR in RV-infected cells using qRT-PCR and Western blotting, respectively. Furthermore, we silenced or overexpressed CaSR in Caco-2 cells using siRNA or a CaSR gene contained adenovirus (Adv-CaSR). qRT-PCR, plaque assay, and Western blotting were used to determine the synthesis of virus genomic RNA, production of progeny virion, and the levels of viral proteins. The content of Ca2+ in cells was observed under confocal microscopy. Results: Compared with control cells, the RV-infected cells presented significantly decreased CaSR expression. Moreover, adenoviral-mediated over-expression or induction of CaSR by R568 greatly inhibited the RV RNA synthesis, protein expression, and formation of viroplasm plaques, thereby suppressing RV replication. In contrast, CaSR-silenced cells exhibited significantly enhanced RV replication. Compared with the Adv-Control group, the concentration of cytosolic Ca2+ significantly decreased in the Adv-CaSR group. Conclusion: These findings demonstrated that CaSR is a potential target for inhibition of RV replication. Therefore, enhancing the expression of CaSR might protect hosts from RV infections. |
| first_indexed | 2024-04-13T17:29:57Z |
| format | Article |
| id | doaj.art-ccfee6ec3f164f53aacf6fc647fc16d4 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T17:29:57Z |
| publishDate | 2022-08-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-ccfee6ec3f164f53aacf6fc647fc16d42022-12-22T02:37:37ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-08-01258997100110.22038/ijbms.2022.64533.1420120706Calcium-sensing receptor acts as an antiviral factor for rotavirus infections and participates in cellular antiviral responseHaohai Huang0Dan Liao1Bin He2Yejia Cui3Rong Pu4Guanghui Zhou5Department of Clinical Pharmacy, SSL Central Hospital of Dongguan, Dongguan Third People’s Hospital, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan, Guangdong, ChinaDepartment of Gynaecology, SSL Central Hospital of Dongguan, Dongguan Third People’s Hospital, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan, Guangdong, ChinaCentral Laboratory, SSL Central Hospital of Dongguan, Dongguan Third People’s Hospital, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan, Guangdong, ChinaDepartment of Clinical Laboratory, SSL Central Hospital of Dongguan City, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan, Guangdong, ChinaDepartment of Clinical Laboratory, SSL Central Hospital of Dongguan City, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan, Guangdong, ChinaDepartment of Rehabilitation medicine, SSL Central Hospital of Dongguan, Dongguan Third People’s Hospital, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan, Guangdong, ChinaObjective(s): Rotavirus (RV) is one of the most significant pathogens associated with childhood diarrhoeal deaths worldwide. Elevated cytoplasmic calcium is required for RV replication, but the underlying mechanisms responsible for calcium influx remain poorly understood. The Calcium-sensing receptor (CaSR) is an important Ca2+ sensor that regulates the transport of Ca2+ into or out of the extracellular space by affecting the status of Ca2+ ion channels on the membrane of cells. Currently, the function of CaSR in RV replication is unclear. Materials and Methods: We evaluated the mRNA and protein levels of CaSR in RV-infected cells using qRT-PCR and Western blotting, respectively. Furthermore, we silenced or overexpressed CaSR in Caco-2 cells using siRNA or a CaSR gene contained adenovirus (Adv-CaSR). qRT-PCR, plaque assay, and Western blotting were used to determine the synthesis of virus genomic RNA, production of progeny virion, and the levels of viral proteins. The content of Ca2+ in cells was observed under confocal microscopy. Results: Compared with control cells, the RV-infected cells presented significantly decreased CaSR expression. Moreover, adenoviral-mediated over-expression or induction of CaSR by R568 greatly inhibited the RV RNA synthesis, protein expression, and formation of viroplasm plaques, thereby suppressing RV replication. In contrast, CaSR-silenced cells exhibited significantly enhanced RV replication. Compared with the Adv-Control group, the concentration of cytosolic Ca2+ significantly decreased in the Adv-CaSR group. Conclusion: These findings demonstrated that CaSR is a potential target for inhibition of RV replication. Therefore, enhancing the expression of CaSR might protect hosts from RV infections.https://ijbms.mums.ac.ir/article_20706_4d3e1b419318a79562e68c18121f6b9d.pdfcalcium-sensing receptordiarrheainfectionreplicationrotavirus |
| spellingShingle | Haohai Huang Dan Liao Bin He Yejia Cui Rong Pu Guanghui Zhou Calcium-sensing receptor acts as an antiviral factor for rotavirus infections and participates in cellular antiviral response Iranian Journal of Basic Medical Sciences calcium-sensing receptor diarrhea infection replication rotavirus |
| title | Calcium-sensing receptor acts as an antiviral factor for rotavirus infections and participates in cellular antiviral response |
| title_full | Calcium-sensing receptor acts as an antiviral factor for rotavirus infections and participates in cellular antiviral response |
| title_fullStr | Calcium-sensing receptor acts as an antiviral factor for rotavirus infections and participates in cellular antiviral response |
| title_full_unstemmed | Calcium-sensing receptor acts as an antiviral factor for rotavirus infections and participates in cellular antiviral response |
| title_short | Calcium-sensing receptor acts as an antiviral factor for rotavirus infections and participates in cellular antiviral response |
| title_sort | calcium sensing receptor acts as an antiviral factor for rotavirus infections and participates in cellular antiviral response |
| topic | calcium-sensing receptor diarrhea infection replication rotavirus |
| url | https://ijbms.mums.ac.ir/article_20706_4d3e1b419318a79562e68c18121f6b9d.pdf |
| work_keys_str_mv | AT haohaihuang calciumsensingreceptoractsasanantiviralfactorforrotavirusinfectionsandparticipatesincellularantiviralresponse AT danliao calciumsensingreceptoractsasanantiviralfactorforrotavirusinfectionsandparticipatesincellularantiviralresponse AT binhe calciumsensingreceptoractsasanantiviralfactorforrotavirusinfectionsandparticipatesincellularantiviralresponse AT yejiacui calciumsensingreceptoractsasanantiviralfactorforrotavirusinfectionsandparticipatesincellularantiviralresponse AT rongpu calciumsensingreceptoractsasanantiviralfactorforrotavirusinfectionsandparticipatesincellularantiviralresponse AT guanghuizhou calciumsensingreceptoractsasanantiviralfactorforrotavirusinfectionsandparticipatesincellularantiviralresponse |