Induction, Multiplication, and Evaluation of Antioxidant Activity of <i>Polyalthia bullata</i> Callus, a Woody Medicinal Plant
<i>Polyalthia bullata</i> is an endangered medicinal plant species. Hence, establishment of <i>P. bullata</i> callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Muras...
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MDPI AG
2020-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/12/1772 |
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| author | Munirah Adibah Kamarul Zaman Azzreena Mohamad Azzeme Illy Kamaliah Ramle Nurfazlinyana Normanshah Siti Nurhafizah Ramli Noor Azmi Shaharuddin Syahida Ahmad Siti Nor Akmar Abdullah |
| author_facet | Munirah Adibah Kamarul Zaman Azzreena Mohamad Azzeme Illy Kamaliah Ramle Nurfazlinyana Normanshah Siti Nurhafizah Ramli Noor Azmi Shaharuddin Syahida Ahmad Siti Nor Akmar Abdullah |
| author_sort | Munirah Adibah Kamarul Zaman |
| collection | DOAJ |
| description | <i>Polyalthia bullata</i> is an endangered medicinal plant species. Hence, establishment of <i>P. bullata</i> callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on <i>P. bullata</i> callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC<sub>50</sub> of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in <i>P. bullata</i> callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the <i>P. bullata</i> callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production. |
| first_indexed | 2024-03-10T14:05:18Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:05:18Z |
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| spelling | doaj.art-cd01bdaae7be4c13802835d111986aec2023-11-21T00:47:18ZengMDPI AGPlants2223-77472020-12-01912177210.3390/plants9121772Induction, Multiplication, and Evaluation of Antioxidant Activity of <i>Polyalthia bullata</i> Callus, a Woody Medicinal PlantMunirah Adibah Kamarul Zaman0Azzreena Mohamad Azzeme1Illy Kamaliah Ramle2Nurfazlinyana Normanshah3Siti Nurhafizah Ramli4Noor Azmi Shaharuddin5Syahida Ahmad6Siti Nor Akmar Abdullah7Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Seri Kembangan 43400, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Seri Kembangan 43400, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Seri Kembangan 43400, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Seri Kembangan 43400, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Seri Kembangan 43400, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Seri Kembangan 43400, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Seri Kembangan 43400, MalaysiaInstitute of Plantation Studies, Universiti Putra Malaysia, Selangor, Seri Kembangan 43400, Malaysia<i>Polyalthia bullata</i> is an endangered medicinal plant species. Hence, establishment of <i>P. bullata</i> callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on <i>P. bullata</i> callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC<sub>50</sub> of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in <i>P. bullata</i> callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the <i>P. bullata</i> callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production.https://www.mdpi.com/2223-7747/9/12/1772<i>Polyalthia bullata</i>callus inductioncallus multiplicationauxinstotal phenolic contenttotal flavonoid content |
| spellingShingle | Munirah Adibah Kamarul Zaman Azzreena Mohamad Azzeme Illy Kamaliah Ramle Nurfazlinyana Normanshah Siti Nurhafizah Ramli Noor Azmi Shaharuddin Syahida Ahmad Siti Nor Akmar Abdullah Induction, Multiplication, and Evaluation of Antioxidant Activity of <i>Polyalthia bullata</i> Callus, a Woody Medicinal Plant Plants <i>Polyalthia bullata</i> callus induction callus multiplication auxins total phenolic content total flavonoid content |
| title | Induction, Multiplication, and Evaluation of Antioxidant Activity of <i>Polyalthia bullata</i> Callus, a Woody Medicinal Plant |
| title_full | Induction, Multiplication, and Evaluation of Antioxidant Activity of <i>Polyalthia bullata</i> Callus, a Woody Medicinal Plant |
| title_fullStr | Induction, Multiplication, and Evaluation of Antioxidant Activity of <i>Polyalthia bullata</i> Callus, a Woody Medicinal Plant |
| title_full_unstemmed | Induction, Multiplication, and Evaluation of Antioxidant Activity of <i>Polyalthia bullata</i> Callus, a Woody Medicinal Plant |
| title_short | Induction, Multiplication, and Evaluation of Antioxidant Activity of <i>Polyalthia bullata</i> Callus, a Woody Medicinal Plant |
| title_sort | induction multiplication and evaluation of antioxidant activity of i polyalthia bullata i callus a woody medicinal plant |
| topic | <i>Polyalthia bullata</i> callus induction callus multiplication auxins total phenolic content total flavonoid content |
| url | https://www.mdpi.com/2223-7747/9/12/1772 |
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