Factors influencing the high rejection rates of HIV 1/2 serology samples at Charlotte Maxeke Johannesburg Academic Hospital and the cost implications
Background: HIV enzyme-linked immunosorbent assay (ELISA) is one of the most requested test sets within Virology and forms an essential part of patient management. Assessment of the rejection criteria is a key quality indicator, crucial for improving laboratory services and efficiency to ensure accu...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AOSIS
2022-01-01
|
| Series: | Southern African Journal of HIV Medicine |
| Subjects: | |
| Online Access: | https://sajhivmed.org.za/index.php/hivmed/article/view/1326 |
| _version_ | 1818013420967952384 |
|---|---|
| author | Bhaveshan Reddy Naseem Cassim Florette Treurnicht Zinhle Makatini |
| author_facet | Bhaveshan Reddy Naseem Cassim Florette Treurnicht Zinhle Makatini |
| author_sort | Bhaveshan Reddy |
| collection | DOAJ |
| description | Background: HIV enzyme-linked immunosorbent assay (ELISA) is one of the most requested test sets within Virology and forms an essential part of patient management. Assessment of the rejection criteria is a key quality indicator, crucial for improving laboratory services and efficiency to ensure accurate and reliable results.
Objectives: The aim of this study was to identify the factors that influence the HIV 1/2 serology rejection rates (RR) at Charlotte Maxeke Johannesburg Academic Hospital and to evaluate the associated costs.
Methods: A retrospective study was conducted (June to December 2019) to identify the RR and rejection criteria of HIV serology samples throughout the total testing process. Descriptive analysis using percentages and frequencies was used to analyse the RR by phase, health establishment, ward and healthcare professional. A cost analysis incorporating minor and major costs was modelled in each phase of testing, and the total cost of rejections was calculated.
Results: A total of 6678 tests were received, and 738 were rejected (RR = 11.1%). The pre-analytical phase contributed significantly to the overall RR, with the requirement of a separate sample (57.44%) the most common reason for rejection. The total cost per rejected test was $2.47, which amounted to a total rejection cost of $197.55, of which $158.18 was caused by the pre-analytical rejection criteria.
Conclusion: High RR of HIV tests were noted, resulting in significant cost wastage. Identification and analysis of rejections must be implemented across all laboratories to improve the efficiency of testing, provide a cost-saving benefit and maintain high laboratory standards. |
| first_indexed | 2024-04-14T06:32:56Z |
| format | Article |
| id | doaj.art-cd02f44c6d614e7592c1ec6a146f6339 |
| institution | Directory Open Access Journal |
| issn | 1608-9693 2078-6751 |
| language | English |
| last_indexed | 2024-04-14T06:32:56Z |
| publishDate | 2022-01-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Southern African Journal of HIV Medicine |
| spelling | doaj.art-cd02f44c6d614e7592c1ec6a146f63392022-12-22T02:07:34ZengAOSISSouthern African Journal of HIV Medicine1608-96932078-67512022-01-01231e1e610.4102/sajhivmed.v23i1.1326782Factors influencing the high rejection rates of HIV 1/2 serology samples at Charlotte Maxeke Johannesburg Academic Hospital and the cost implicationsBhaveshan Reddy0Naseem Cassim1Florette Treurnicht2Zinhle Makatini3Department of Virology, Faculty of Health Science, University of the Witwatersrand, Johannesburg, South Africa; and, National Health Laboratory Service, JohannesburgNational Health Laboratory Service, Johannesburg, South Africa; and, Department of Haematology and Molecular Medicine, Faculty of Health Sciences, University of the Witwatersrand, JohannesburgDepartment of Virology, Faculty of Health Science, University of the Witwatersrand, Johannesburg, South Africa; and, National Health Laboratory Service, JohannesburgDepartment of Virology, Faculty of Health Science, University of the Witwatersrand, Johannesburg, South Africa; and, National Health Laboratory Service, JohannesburgBackground: HIV enzyme-linked immunosorbent assay (ELISA) is one of the most requested test sets within Virology and forms an essential part of patient management. Assessment of the rejection criteria is a key quality indicator, crucial for improving laboratory services and efficiency to ensure accurate and reliable results. Objectives: The aim of this study was to identify the factors that influence the HIV 1/2 serology rejection rates (RR) at Charlotte Maxeke Johannesburg Academic Hospital and to evaluate the associated costs. Methods: A retrospective study was conducted (June to December 2019) to identify the RR and rejection criteria of HIV serology samples throughout the total testing process. Descriptive analysis using percentages and frequencies was used to analyse the RR by phase, health establishment, ward and healthcare professional. A cost analysis incorporating minor and major costs was modelled in each phase of testing, and the total cost of rejections was calculated. Results: A total of 6678 tests were received, and 738 were rejected (RR = 11.1%). The pre-analytical phase contributed significantly to the overall RR, with the requirement of a separate sample (57.44%) the most common reason for rejection. The total cost per rejected test was $2.47, which amounted to a total rejection cost of $197.55, of which $158.18 was caused by the pre-analytical rejection criteria. Conclusion: High RR of HIV tests were noted, resulting in significant cost wastage. Identification and analysis of rejections must be implemented across all laboratories to improve the efficiency of testing, provide a cost-saving benefit and maintain high laboratory standards.https://sajhivmed.org.za/index.php/hivmed/article/view/1326hivrejection ratescost analysislaboratorydiagnostics |
| spellingShingle | Bhaveshan Reddy Naseem Cassim Florette Treurnicht Zinhle Makatini Factors influencing the high rejection rates of HIV 1/2 serology samples at Charlotte Maxeke Johannesburg Academic Hospital and the cost implications Southern African Journal of HIV Medicine hiv rejection rates cost analysis laboratory diagnostics |
| title | Factors influencing the high rejection rates of HIV 1/2 serology samples at Charlotte Maxeke Johannesburg Academic Hospital and the cost implications |
| title_full | Factors influencing the high rejection rates of HIV 1/2 serology samples at Charlotte Maxeke Johannesburg Academic Hospital and the cost implications |
| title_fullStr | Factors influencing the high rejection rates of HIV 1/2 serology samples at Charlotte Maxeke Johannesburg Academic Hospital and the cost implications |
| title_full_unstemmed | Factors influencing the high rejection rates of HIV 1/2 serology samples at Charlotte Maxeke Johannesburg Academic Hospital and the cost implications |
| title_short | Factors influencing the high rejection rates of HIV 1/2 serology samples at Charlotte Maxeke Johannesburg Academic Hospital and the cost implications |
| title_sort | factors influencing the high rejection rates of hiv 1 2 serology samples at charlotte maxeke johannesburg academic hospital and the cost implications |
| topic | hiv rejection rates cost analysis laboratory diagnostics |
| url | https://sajhivmed.org.za/index.php/hivmed/article/view/1326 |
| work_keys_str_mv | AT bhaveshanreddy factorsinfluencingthehighrejectionratesofhiv12serologysamplesatcharlottemaxekejohannesburgacademichospitalandthecostimplications AT naseemcassim factorsinfluencingthehighrejectionratesofhiv12serologysamplesatcharlottemaxekejohannesburgacademichospitalandthecostimplications AT florettetreurnicht factorsinfluencingthehighrejectionratesofhiv12serologysamplesatcharlottemaxekejohannesburgacademichospitalandthecostimplications AT zinhlemakatini factorsinfluencingthehighrejectionratesofhiv12serologysamplesatcharlottemaxekejohannesburgacademichospitalandthecostimplications |