The inhibitory effects of Staphylococcus aureus on the antibiotic susceptibility and virulence factors of Pseudomonas aeruginosa: A549 cell line model
Abstract Pseudomonas aeruginosa and Staphylococcus aureus often lead to serious lung infections. This study aimed to investigate the role of S. aureus in the expression of the β-lactamase enzymes and virulence factors of P. aeruginosa in the polymicrobial infections of the respiratory tract. Biofilm...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-03-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-021-01210-y |
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| author | Sanaz Dehbashi Mohammad Yousef Alikhani Hamed Tahmasebi Mohammad Reza Arabestani |
| author_facet | Sanaz Dehbashi Mohammad Yousef Alikhani Hamed Tahmasebi Mohammad Reza Arabestani |
| author_sort | Sanaz Dehbashi |
| collection | DOAJ |
| description | Abstract Pseudomonas aeruginosa and Staphylococcus aureus often lead to serious lung infections. This study aimed to investigate the role of S. aureus in the expression of the β-lactamase enzymes and virulence factors of P. aeruginosa in the polymicrobial infections of the respiratory tract. Biofilm and planktonic co-culture of P. aeruginosa and S. aureus were performed in the A549 cell line. Then, antibiotic resistance and virulence factors of P. aeruginosa were examined, and the expression of lasR, lasI, algD, mexR, and KPC genes were determined using qPCR. S.aureus decreased β-lactam resistance but increased resistance to tobramycin in the biofilm condition. Furthermore, S.aureus showed a positive effect on reducing resistance to meropenem, doripenem, and tobramycin (except PA-2). Altough it was demonstrated that S.aureus reduced the viability of P. aeruginosa, particularly in the biofilm state, the pathogenicity of the recovered strains of P.aeruginosa increased. Moreover, the gene expression levels for lasR/I and algD were increased in biofilm conditions. The levels of lasI were more prominent in the virulent strain than the β-lactamase producing strain. Furthermore, the expression of KPC was increased in all strains of P. aeruginosa. According to the findings of this study, S. aureus has an inhibitory effect in polymicrobial infections by suppressing the β-lactamase genes and viability of P. aeruginosa. Also, it cooperates with the biofilm-producing P. aeruginosa strains to increase pathogenicity and resistance to tobramycin. |
| first_indexed | 2024-12-14T17:50:45Z |
| format | Article |
| id | doaj.art-cd04c694b74b43299309c1669f6c4068 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-14T17:50:45Z |
| publishDate | 2021-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-cd04c694b74b43299309c1669f6c40682022-12-21T22:52:39ZengSpringerOpenAMB Express2191-08552021-03-0111111010.1186/s13568-021-01210-yThe inhibitory effects of Staphylococcus aureus on the antibiotic susceptibility and virulence factors of Pseudomonas aeruginosa: A549 cell line modelSanaz Dehbashi0Mohammad Yousef Alikhani1Hamed Tahmasebi2Mohammad Reza Arabestani3Department of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesSchool of Medicine, Shahroud University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesAbstract Pseudomonas aeruginosa and Staphylococcus aureus often lead to serious lung infections. This study aimed to investigate the role of S. aureus in the expression of the β-lactamase enzymes and virulence factors of P. aeruginosa in the polymicrobial infections of the respiratory tract. Biofilm and planktonic co-culture of P. aeruginosa and S. aureus were performed in the A549 cell line. Then, antibiotic resistance and virulence factors of P. aeruginosa were examined, and the expression of lasR, lasI, algD, mexR, and KPC genes were determined using qPCR. S.aureus decreased β-lactam resistance but increased resistance to tobramycin in the biofilm condition. Furthermore, S.aureus showed a positive effect on reducing resistance to meropenem, doripenem, and tobramycin (except PA-2). Altough it was demonstrated that S.aureus reduced the viability of P. aeruginosa, particularly in the biofilm state, the pathogenicity of the recovered strains of P.aeruginosa increased. Moreover, the gene expression levels for lasR/I and algD were increased in biofilm conditions. The levels of lasI were more prominent in the virulent strain than the β-lactamase producing strain. Furthermore, the expression of KPC was increased in all strains of P. aeruginosa. According to the findings of this study, S. aureus has an inhibitory effect in polymicrobial infections by suppressing the β-lactamase genes and viability of P. aeruginosa. Also, it cooperates with the biofilm-producing P. aeruginosa strains to increase pathogenicity and resistance to tobramycin.https://doi.org/10.1186/s13568-021-01210-yStaphylococcus aureusPseudomonas aeruginosaPolymicrobial infectionsRespiratory TractResistanceVirulence |
| spellingShingle | Sanaz Dehbashi Mohammad Yousef Alikhani Hamed Tahmasebi Mohammad Reza Arabestani The inhibitory effects of Staphylococcus aureus on the antibiotic susceptibility and virulence factors of Pseudomonas aeruginosa: A549 cell line model AMB Express Staphylococcus aureus Pseudomonas aeruginosa Polymicrobial infections Respiratory Tract Resistance Virulence |
| title | The inhibitory effects of Staphylococcus aureus on the antibiotic susceptibility and virulence factors of Pseudomonas aeruginosa: A549 cell line model |
| title_full | The inhibitory effects of Staphylococcus aureus on the antibiotic susceptibility and virulence factors of Pseudomonas aeruginosa: A549 cell line model |
| title_fullStr | The inhibitory effects of Staphylococcus aureus on the antibiotic susceptibility and virulence factors of Pseudomonas aeruginosa: A549 cell line model |
| title_full_unstemmed | The inhibitory effects of Staphylococcus aureus on the antibiotic susceptibility and virulence factors of Pseudomonas aeruginosa: A549 cell line model |
| title_short | The inhibitory effects of Staphylococcus aureus on the antibiotic susceptibility and virulence factors of Pseudomonas aeruginosa: A549 cell line model |
| title_sort | inhibitory effects of staphylococcus aureus on the antibiotic susceptibility and virulence factors of pseudomonas aeruginosa a549 cell line model |
| topic | Staphylococcus aureus Pseudomonas aeruginosa Polymicrobial infections Respiratory Tract Resistance Virulence |
| url | https://doi.org/10.1186/s13568-021-01210-y |
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