Comparative physiological and transcriptomic analyses reveal the mechanisms of CO2 enrichment in promoting the growth and quality in Lactuca sativa.
The increase in the concentration of CO2 in the atmosphere has attracted widespread attention. To explore the effect of elevated CO2 on lettuce growth and better understand the mechanism of elevated CO2 in lettuce cultivation, 3 kinds of lettuce with 4 real leaves were selected and planted in a sola...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0278159 |
| _version_ | 1811168429214793728 |
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| author | Hongxia Song Peiqi Wu Xiaonan Lu Bei Wang Tianyue Song Qiang Lu Meilan Li Xiaoyong Xu |
| author_facet | Hongxia Song Peiqi Wu Xiaonan Lu Bei Wang Tianyue Song Qiang Lu Meilan Li Xiaoyong Xu |
| author_sort | Hongxia Song |
| collection | DOAJ |
| description | The increase in the concentration of CO2 in the atmosphere has attracted widespread attention. To explore the effect of elevated CO2 on lettuce growth and better understand the mechanism of elevated CO2 in lettuce cultivation, 3 kinds of lettuce with 4 real leaves were selected and planted in a solar greenhouse. One week later, CO2 was applied from 8:00 a.m. to 10:00 a.m. on sunny days for 30 days. The results showed that the growth potential of lettuce was enhanced under CO2 enrichment. The content of vitamin C and chlorophyll in the three lettuce varieties increased, and the content of nitrate nitrogen decreased. The light saturation point and net photosynthetic rate of leaves increased, and the light compensation point decreased. Transcriptome analysis showed that there were 217 differentially expressed genes (DEGs) shared by the three varieties, among which 166 were upregulated, 44 were downregulated, and 7 DEGs were inconsistent in the three materials. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that these DEGs involved mainly the ethylene signaling pathway, jasmonic acid signaling pathway, porphyrin and chlorophyll metabolism pathway, starch and sucrose metabolism pathway, etc. Forty-one DEGs in response to CO2 enrichment were screened out by Gene Ontology (GO) analysis, and the biological processes involved were consistent with KEGG analysis. which suggested that the growth and nutritional quality of lettuce could be improved by increasing the enzyme activity and gene expression levels of photosynthesis, hormone signaling and carbohydrate metabolism. The results laid a theoretical foundation for lettuce cultivation in solar greenhouses and the application of CO2 fertilization technology. |
| first_indexed | 2024-04-10T16:26:12Z |
| format | Article |
| id | doaj.art-cd076f1ecbda4e0e90083ab9304cdf63 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T16:26:12Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cd076f1ecbda4e0e90083ab9304cdf632023-02-09T05:32:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01182e027815910.1371/journal.pone.0278159Comparative physiological and transcriptomic analyses reveal the mechanisms of CO2 enrichment in promoting the growth and quality in Lactuca sativa.Hongxia SongPeiqi WuXiaonan LuBei WangTianyue SongQiang LuMeilan LiXiaoyong XuThe increase in the concentration of CO2 in the atmosphere has attracted widespread attention. To explore the effect of elevated CO2 on lettuce growth and better understand the mechanism of elevated CO2 in lettuce cultivation, 3 kinds of lettuce with 4 real leaves were selected and planted in a solar greenhouse. One week later, CO2 was applied from 8:00 a.m. to 10:00 a.m. on sunny days for 30 days. The results showed that the growth potential of lettuce was enhanced under CO2 enrichment. The content of vitamin C and chlorophyll in the three lettuce varieties increased, and the content of nitrate nitrogen decreased. The light saturation point and net photosynthetic rate of leaves increased, and the light compensation point decreased. Transcriptome analysis showed that there were 217 differentially expressed genes (DEGs) shared by the three varieties, among which 166 were upregulated, 44 were downregulated, and 7 DEGs were inconsistent in the three materials. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that these DEGs involved mainly the ethylene signaling pathway, jasmonic acid signaling pathway, porphyrin and chlorophyll metabolism pathway, starch and sucrose metabolism pathway, etc. Forty-one DEGs in response to CO2 enrichment were screened out by Gene Ontology (GO) analysis, and the biological processes involved were consistent with KEGG analysis. which suggested that the growth and nutritional quality of lettuce could be improved by increasing the enzyme activity and gene expression levels of photosynthesis, hormone signaling and carbohydrate metabolism. The results laid a theoretical foundation for lettuce cultivation in solar greenhouses and the application of CO2 fertilization technology.https://doi.org/10.1371/journal.pone.0278159 |
| spellingShingle | Hongxia Song Peiqi Wu Xiaonan Lu Bei Wang Tianyue Song Qiang Lu Meilan Li Xiaoyong Xu Comparative physiological and transcriptomic analyses reveal the mechanisms of CO2 enrichment in promoting the growth and quality in Lactuca sativa. PLoS ONE |
| title | Comparative physiological and transcriptomic analyses reveal the mechanisms of CO2 enrichment in promoting the growth and quality in Lactuca sativa. |
| title_full | Comparative physiological and transcriptomic analyses reveal the mechanisms of CO2 enrichment in promoting the growth and quality in Lactuca sativa. |
| title_fullStr | Comparative physiological and transcriptomic analyses reveal the mechanisms of CO2 enrichment in promoting the growth and quality in Lactuca sativa. |
| title_full_unstemmed | Comparative physiological and transcriptomic analyses reveal the mechanisms of CO2 enrichment in promoting the growth and quality in Lactuca sativa. |
| title_short | Comparative physiological and transcriptomic analyses reveal the mechanisms of CO2 enrichment in promoting the growth and quality in Lactuca sativa. |
| title_sort | comparative physiological and transcriptomic analyses reveal the mechanisms of co2 enrichment in promoting the growth and quality in lactuca sativa |
| url | https://doi.org/10.1371/journal.pone.0278159 |
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