Experimental transmission of ovine atypical scrapie to cattle
Abstract Classical bovine spongiform encephalopathy (BSE) in cattle was caused by the recycling and feeding of meat and bone meal contaminated with a transmissible spongiform encephalopathy (TSE) agent but its origin remains unknown. This study aimed to determine whether atypical scrapie could cause...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-023-01224-3 |
| _version_ | 1827633414894780416 |
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| author | Timm Konold John Spiropoulos Janet Hills Hasina Abdul Saira Cawthraw Laura Phelan Amy McKenna Lauren Read Sara Canoyra Alba Marín-Moreno Juan María Torres |
| author_facet | Timm Konold John Spiropoulos Janet Hills Hasina Abdul Saira Cawthraw Laura Phelan Amy McKenna Lauren Read Sara Canoyra Alba Marín-Moreno Juan María Torres |
| author_sort | Timm Konold |
| collection | DOAJ |
| description | Abstract Classical bovine spongiform encephalopathy (BSE) in cattle was caused by the recycling and feeding of meat and bone meal contaminated with a transmissible spongiform encephalopathy (TSE) agent but its origin remains unknown. This study aimed to determine whether atypical scrapie could cause disease in cattle and to compare it with other known TSEs in cattle. Two groups of calves (five and two) were intracerebrally inoculated with atypical scrapie brain homogenate from two sheep with atypical scrapie. Controls were five calves intracerebrally inoculated with saline solution and one non-inoculated animal. Cattle were clinically monitored until clinical end-stage or at least 96 months post-inoculation (mpi). After euthanasia, tissues were collected for TSE diagnosis and potential transgenic mouse bioassay. One animal was culled with BSE-like clinical signs at 48 mpi. The other cattle either developed intercurrent diseases leading to cull or remained clinical unremarkable at study endpoint, including control cattle. None of the animals tested positive for TSEs by Western immunoblot and immunohistochemistry. Bioassay of brain samples from the clinical suspect in Ov-Tg338 and Bov-Tg110 mice was also negative. By contrast, protein misfolding cyclic amplification detected prions in the examined brains from atypical scrapie-challenged cattle, which had a classical BSE-like phenotype. This study demonstrates for the first time that a TSE agent with BSE-like properties can be amplified in cattle inoculated with atypical scrapie brain homogenate. |
| first_indexed | 2024-03-09T14:59:25Z |
| format | Article |
| id | doaj.art-cd0bc6d5b7294b23ba2c9edbd652c074 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-03-09T14:59:25Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-cd0bc6d5b7294b23ba2c9edbd652c0742023-11-26T13:59:55ZengBMCVeterinary Research1297-97162023-10-0154111310.1186/s13567-023-01224-3Experimental transmission of ovine atypical scrapie to cattleTimm Konold0John Spiropoulos1Janet Hills2Hasina Abdul3Saira Cawthraw4Laura Phelan5Amy McKenna6Lauren Read7Sara Canoyra8Alba Marín-Moreno9Juan María Torres10Department of Pathology and Animal Sciences, Animal & Plant Health Agency WeybridgeDepartment of Pathology and Animal Sciences, Animal & Plant Health Agency WeybridgeDepartment of Pathology and Animal Sciences, Animal & Plant Health Agency WeybridgeDepartment of Pathology and Animal Sciences, Animal & Plant Health Agency WeybridgeCentral Unit for Sequencing and PCR, Animal & Plant Health Agency WeybridgeDepartment of Pathology and Animal Sciences, Animal & Plant Health Agency WeybridgeDepartment of Pathology and Animal Sciences, Animal & Plant Health Agency WeybridgeDepartment of Pathology and Animal Sciences, Animal & Plant Health Agency WeybridgeCentro de Investigación en Sanidad Animal (CISA-INIA-CSIC)Centro de Investigación en Sanidad Animal (CISA-INIA-CSIC)Centro de Investigación en Sanidad Animal (CISA-INIA-CSIC)Abstract Classical bovine spongiform encephalopathy (BSE) in cattle was caused by the recycling and feeding of meat and bone meal contaminated with a transmissible spongiform encephalopathy (TSE) agent but its origin remains unknown. This study aimed to determine whether atypical scrapie could cause disease in cattle and to compare it with other known TSEs in cattle. Two groups of calves (five and two) were intracerebrally inoculated with atypical scrapie brain homogenate from two sheep with atypical scrapie. Controls were five calves intracerebrally inoculated with saline solution and one non-inoculated animal. Cattle were clinically monitored until clinical end-stage or at least 96 months post-inoculation (mpi). After euthanasia, tissues were collected for TSE diagnosis and potential transgenic mouse bioassay. One animal was culled with BSE-like clinical signs at 48 mpi. The other cattle either developed intercurrent diseases leading to cull or remained clinical unremarkable at study endpoint, including control cattle. None of the animals tested positive for TSEs by Western immunoblot and immunohistochemistry. Bioassay of brain samples from the clinical suspect in Ov-Tg338 and Bov-Tg110 mice was also negative. By contrast, protein misfolding cyclic amplification detected prions in the examined brains from atypical scrapie-challenged cattle, which had a classical BSE-like phenotype. This study demonstrates for the first time that a TSE agent with BSE-like properties can be amplified in cattle inoculated with atypical scrapie brain homogenate.https://doi.org/10.1186/s13567-023-01224-3Transmissible spongiform encephalopathyprionatypical scrapiebovinePMCAmouse bioassay |
| spellingShingle | Timm Konold John Spiropoulos Janet Hills Hasina Abdul Saira Cawthraw Laura Phelan Amy McKenna Lauren Read Sara Canoyra Alba Marín-Moreno Juan María Torres Experimental transmission of ovine atypical scrapie to cattle Veterinary Research Transmissible spongiform encephalopathy prion atypical scrapie bovine PMCA mouse bioassay |
| title | Experimental transmission of ovine atypical scrapie to cattle |
| title_full | Experimental transmission of ovine atypical scrapie to cattle |
| title_fullStr | Experimental transmission of ovine atypical scrapie to cattle |
| title_full_unstemmed | Experimental transmission of ovine atypical scrapie to cattle |
| title_short | Experimental transmission of ovine atypical scrapie to cattle |
| title_sort | experimental transmission of ovine atypical scrapie to cattle |
| topic | Transmissible spongiform encephalopathy prion atypical scrapie bovine PMCA mouse bioassay |
| url | https://doi.org/10.1186/s13567-023-01224-3 |
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