The Application of Microfluidic Technologies in Aptamer Selection
Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligan...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.730035/full |
| _version_ | 1819094484073316352 |
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| author | Yang Liu Yang Liu Nijia Wang Nijia Wang Chiu-Wing Chan Aiping Lu Aiping Lu Yuanyuan Yu Yuanyuan Yu Ge Zhang Ge Zhang Kangning Ren Kangning Ren Kangning Ren Kangning Ren |
| author_facet | Yang Liu Yang Liu Nijia Wang Nijia Wang Chiu-Wing Chan Aiping Lu Aiping Lu Yuanyuan Yu Yuanyuan Yu Ge Zhang Ge Zhang Kangning Ren Kangning Ren Kangning Ren Kangning Ren |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligands by exponential enrichment (SELEX). Numerous SELEX methods have been developed for aptamer selections. However, the conventional SELEX methods still suffer from high labor intensity, low operation efficiency, and low success rate. Thus, the applications of aptamer with desired properties are limited. With their advantages of low cost, high speed, and upgraded extent of automation, microfluidic technologies have become promising tools for rapid and high throughput aptamer selection. This paper reviews current progresses of such microfluidic systems for aptamer selection. Comparisons of selection performances with discussions on principles, structure, operations, as well as advantages and limitations of various microfluidic-based aptamer selection methods are provided. |
| first_indexed | 2024-12-21T23:28:08Z |
| format | Article |
| id | doaj.art-cd0beeba1637487c931ba40dcf57b78e |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-21T23:28:08Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-cd0beeba1637487c931ba40dcf57b78e2022-12-21T18:46:34ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-09-01910.3389/fcell.2021.730035730035The Application of Microfluidic Technologies in Aptamer SelectionYang Liu0Yang Liu1Nijia Wang2Nijia Wang3Chiu-Wing Chan4Aiping Lu5Aiping Lu6Yuanyuan Yu7Yuanyuan Yu8Ge Zhang9Ge Zhang10Kangning Ren11Kangning Ren12Kangning Ren13Kangning Ren14Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong, SAR ChinaGuangdong-Hong Kong Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery, Hong Kong, Hong Kong, SAR ChinaDepartment of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong, SAR ChinaGuangdong-Hong Kong Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery, Hong Kong, Hong Kong, SAR ChinaDepartment of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong, SAR ChinaGuangdong-Hong Kong Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery, Hong Kong, Hong Kong, SAR ChinaSchool of Chinese Medicine, Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases, Hong Kong Baptist University, Hong Kong, Hong Kong, SAR ChinaGuangdong-Hong Kong Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery, Hong Kong, Hong Kong, SAR ChinaSchool of Chinese Medicine, Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases, Hong Kong Baptist University, Hong Kong, Hong Kong, SAR ChinaGuangdong-Hong Kong Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery, Hong Kong, Hong Kong, SAR ChinaSchool of Chinese Medicine, Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases, Hong Kong Baptist University, Hong Kong, Hong Kong, SAR ChinaDepartment of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong, SAR ChinaGuangdong-Hong Kong Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery, Hong Kong, Hong Kong, SAR ChinaInstitute of Research and Continuing Education, Hong Kong Baptist University, Shenzhen, ChinaState Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon, Hong Kong, SAR ChinaAptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligands by exponential enrichment (SELEX). Numerous SELEX methods have been developed for aptamer selections. However, the conventional SELEX methods still suffer from high labor intensity, low operation efficiency, and low success rate. Thus, the applications of aptamer with desired properties are limited. With their advantages of low cost, high speed, and upgraded extent of automation, microfluidic technologies have become promising tools for rapid and high throughput aptamer selection. This paper reviews current progresses of such microfluidic systems for aptamer selection. Comparisons of selection performances with discussions on principles, structure, operations, as well as advantages and limitations of various microfluidic-based aptamer selection methods are provided.https://www.frontiersin.org/articles/10.3389/fcell.2021.730035/fullaptameroligonucleotideSELEXmicrofluidicsmicroarray |
| spellingShingle | Yang Liu Yang Liu Nijia Wang Nijia Wang Chiu-Wing Chan Aiping Lu Aiping Lu Yuanyuan Yu Yuanyuan Yu Ge Zhang Ge Zhang Kangning Ren Kangning Ren Kangning Ren Kangning Ren The Application of Microfluidic Technologies in Aptamer Selection Frontiers in Cell and Developmental Biology aptamer oligonucleotide SELEX microfluidics microarray |
| title | The Application of Microfluidic Technologies in Aptamer Selection |
| title_full | The Application of Microfluidic Technologies in Aptamer Selection |
| title_fullStr | The Application of Microfluidic Technologies in Aptamer Selection |
| title_full_unstemmed | The Application of Microfluidic Technologies in Aptamer Selection |
| title_short | The Application of Microfluidic Technologies in Aptamer Selection |
| title_sort | application of microfluidic technologies in aptamer selection |
| topic | aptamer oligonucleotide SELEX microfluidics microarray |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.730035/full |
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