Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells
Hyperforin (HF), a substance that accumulates in the leaves and flowers of Hypericum perforatum L. (St. John’s wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains. HF exhibits several medicinal properties and is mainly used as an antidepressant. So far, the antigenotoxicity...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/22/1/167 |
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| author | Petronela Imreova Jana Feruszova Stanislav Kyzek Kristina Bodnarova Martina Zduriencikova Katarina Kozics Pavel Mucaji Eliska Galova Andrea Sevcovicova Eva Miadokova Ivan Chalupa |
| author_facet | Petronela Imreova Jana Feruszova Stanislav Kyzek Kristina Bodnarova Martina Zduriencikova Katarina Kozics Pavel Mucaji Eliska Galova Andrea Sevcovicova Eva Miadokova Ivan Chalupa |
| author_sort | Petronela Imreova |
| collection | DOAJ |
| description | Hyperforin (HF), a substance that accumulates in the leaves and flowers of Hypericum perforatum L. (St. John’s wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains. HF exhibits several medicinal properties and is mainly used as an antidepressant. So far, the antigenotoxicity of HF has not been investigated at the level of primary genetic damage, gene mutations, and chromosome aberrations, simultaneously. The present work is designed to investigate the potential antigenotoxic effects of HF using three different experimental test systems. The antigenotoxic effect of HF leading to the decrease of primary/transient promutagenic genetic changes was detected by the alkaline comet assay on human lymphocytes. The HF antimutagenic effect leading to the reduction of gene mutations was assessed using the Ames test on the standard Salmonella typhimurium (TA97, TA98, and TA100) bacterial strains, and the anticlastogenic effect of HF leading to the reduction of chromosome aberrations was evaluated by the in vitro mammalian chromosome aberration test on the human tumor cell line HepG2 and the non-carcinogenic cell line VH10. Our findings provided evidence that HF showed antigenotoxic effects towards oxidative mutagen zeocin in the comet assay and diagnostic mutagen (4-nitroquinoline-1-oxide) in the Ames test. Moreover, HF exhibited an anticlastogenic effect towards benzo(a)pyrene and cisplatin in the chromosome aberration test. |
| first_indexed | 2024-12-19T12:48:35Z |
| format | Article |
| id | doaj.art-cd0d0d62cb78428084a073d45b989d99 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-19T12:48:35Z |
| publishDate | 2017-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cd0d0d62cb78428084a073d45b989d992022-12-21T20:20:39ZengMDPI AGMolecules1420-30492017-01-0122116710.3390/molecules22010167molecules22010167Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial CellsPetronela Imreova0Jana Feruszova1Stanislav Kyzek2Kristina Bodnarova3Martina Zduriencikova4Katarina Kozics5Pavel Mucaji6Eliska Galova7Andrea Sevcovicova8Eva Miadokova9Ivan Chalupa10Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovičova 6, 842 15 Bratislava, SlovakiaDepartment of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovičova 6, 842 15 Bratislava, SlovakiaDepartment of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovičova 6, 842 15 Bratislava, SlovakiaDepartment of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovičova 6, 842 15 Bratislava, SlovakiaCancer Research Institute, Biomedical Research Center, SAS, Dúbravská cesta 9, 845 05 Bratislava, SlovakiaCancer Research Institute, Biomedical Research Center, SAS, Dúbravská cesta 9, 845 05 Bratislava, SlovakiaDepartment of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, SlovakiaDepartment of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovičova 6, 842 15 Bratislava, SlovakiaDepartment of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovičova 6, 842 15 Bratislava, SlovakiaDepartment of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovičova 6, 842 15 Bratislava, SlovakiaCancer Research Institute, Biomedical Research Center, SAS, Dúbravská cesta 9, 845 05 Bratislava, SlovakiaHyperforin (HF), a substance that accumulates in the leaves and flowers of Hypericum perforatum L. (St. John’s wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains. HF exhibits several medicinal properties and is mainly used as an antidepressant. So far, the antigenotoxicity of HF has not been investigated at the level of primary genetic damage, gene mutations, and chromosome aberrations, simultaneously. The present work is designed to investigate the potential antigenotoxic effects of HF using three different experimental test systems. The antigenotoxic effect of HF leading to the decrease of primary/transient promutagenic genetic changes was detected by the alkaline comet assay on human lymphocytes. The HF antimutagenic effect leading to the reduction of gene mutations was assessed using the Ames test on the standard Salmonella typhimurium (TA97, TA98, and TA100) bacterial strains, and the anticlastogenic effect of HF leading to the reduction of chromosome aberrations was evaluated by the in vitro mammalian chromosome aberration test on the human tumor cell line HepG2 and the non-carcinogenic cell line VH10. Our findings provided evidence that HF showed antigenotoxic effects towards oxidative mutagen zeocin in the comet assay and diagnostic mutagen (4-nitroquinoline-1-oxide) in the Ames test. Moreover, HF exhibited an anticlastogenic effect towards benzo(a)pyrene and cisplatin in the chromosome aberration test.http://www.mdpi.com/1420-3049/22/1/167hyperforinantigenotoxicityMTT assaycomet assayAmes testchromosome aberrations test |
| spellingShingle | Petronela Imreova Jana Feruszova Stanislav Kyzek Kristina Bodnarova Martina Zduriencikova Katarina Kozics Pavel Mucaji Eliska Galova Andrea Sevcovicova Eva Miadokova Ivan Chalupa Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells Molecules hyperforin antigenotoxicity MTT assay comet assay Ames test chromosome aberrations test |
| title | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
| title_full | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
| title_fullStr | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
| title_full_unstemmed | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
| title_short | Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells |
| title_sort | hyperforin exhibits antigenotoxic activity on human and bacterial cells |
| topic | hyperforin antigenotoxicity MTT assay comet assay Ames test chromosome aberrations test |
| url | http://www.mdpi.com/1420-3049/22/1/167 |
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