Regulatory Effects of Functional Soluble Dietary Fiber from <i>Saccharina japonica</i> Byproduct on the Liver of Obese Mice with Type 2 Diabetes Mellitus
Though the relationship between dietary fiber and physical health has been investigated widely, the use of dietary fiber from marine plants has been investigated relatively rarely. The <i>Saccharina japonica</i> byproducts after the production of algin contain a large amount of insoluble...
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MDPI AG
2022-01-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/2/91 |
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| author | Liping Zhang Xixi Wang Yingying He Junhan Cao Kai Wang Huan Lin Changfeng Qu Jinlai Miao |
| author_facet | Liping Zhang Xixi Wang Yingying He Junhan Cao Kai Wang Huan Lin Changfeng Qu Jinlai Miao |
| author_sort | Liping Zhang |
| collection | DOAJ |
| description | Though the relationship between dietary fiber and physical health has been investigated widely, the use of dietary fiber from marine plants has been investigated relatively rarely. The <i>Saccharina japonica</i> byproducts after the production of algin contain a large amount of insoluble polysaccharide, which will cause a waste of resources if ignored. Soluble dietary fiber (SDF)prepared from waste byproducts of <i>Saccharina japonica</i> by alkaline hydrolysis method for the first time had a wrinkled microscopic surface and low crystallinity, which not only significantly reduced liver index, serum levels of aspartate aminotransferase (AST) and alanine amiotransferase (ALT), and liver fat accumulation damage to the livers of obese diabetic mice, but also activated the PI3K/AKT signaling pathway to increase liver glycogen synthesis and glycolysis. By LC-MS/MS employing a Nexera UPLC tandem QE high-resolution mass spectrometer, the 6 potential biomarker metabolites were screened, namely glycerophosphocholine (GPC), phosphocholine (PCho), pantothenic acid, glutathione (GSH), oxidized glutathione (GSSG), and betaine; several pathways of these metabolites were associated with lipid metabolism, glycogen metabolism, and amino acid metabolism in the liver were observed. This study further provided a detailed insight into the mechanisms of SDF from <i>Saccharina japonica</i> byproducts in regulating the livers of obese mice with type 2 diabetes and laid a reliable foundation for the further development and utilization of <i>Saccharina japonica</i>. |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T21:33:42Z |
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| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-cd0eb63c39674f11891d322425f65dc92023-11-23T20:49:41ZengMDPI AGMarine Drugs1660-33972022-01-012029110.3390/md20020091Regulatory Effects of Functional Soluble Dietary Fiber from <i>Saccharina japonica</i> Byproduct on the Liver of Obese Mice with Type 2 Diabetes MellitusLiping Zhang0Xixi Wang1Yingying He2Junhan Cao3Kai Wang4Huan Lin5Changfeng Qu6Jinlai Miao7Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao 266071, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resource, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resource, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resource, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resource, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resource, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resource, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resource, Qingdao 266061, ChinaThough the relationship between dietary fiber and physical health has been investigated widely, the use of dietary fiber from marine plants has been investigated relatively rarely. The <i>Saccharina japonica</i> byproducts after the production of algin contain a large amount of insoluble polysaccharide, which will cause a waste of resources if ignored. Soluble dietary fiber (SDF)prepared from waste byproducts of <i>Saccharina japonica</i> by alkaline hydrolysis method for the first time had a wrinkled microscopic surface and low crystallinity, which not only significantly reduced liver index, serum levels of aspartate aminotransferase (AST) and alanine amiotransferase (ALT), and liver fat accumulation damage to the livers of obese diabetic mice, but also activated the PI3K/AKT signaling pathway to increase liver glycogen synthesis and glycolysis. By LC-MS/MS employing a Nexera UPLC tandem QE high-resolution mass spectrometer, the 6 potential biomarker metabolites were screened, namely glycerophosphocholine (GPC), phosphocholine (PCho), pantothenic acid, glutathione (GSH), oxidized glutathione (GSSG), and betaine; several pathways of these metabolites were associated with lipid metabolism, glycogen metabolism, and amino acid metabolism in the liver were observed. This study further provided a detailed insight into the mechanisms of SDF from <i>Saccharina japonica</i> byproducts in regulating the livers of obese mice with type 2 diabetes and laid a reliable foundation for the further development and utilization of <i>Saccharina japonica</i>.https://www.mdpi.com/1660-3397/20/2/91<i>Saccharina japonica</i>dietary fiberPI3K/AKTliver metabolomics |
| spellingShingle | Liping Zhang Xixi Wang Yingying He Junhan Cao Kai Wang Huan Lin Changfeng Qu Jinlai Miao Regulatory Effects of Functional Soluble Dietary Fiber from <i>Saccharina japonica</i> Byproduct on the Liver of Obese Mice with Type 2 Diabetes Mellitus Marine Drugs <i>Saccharina japonica</i> dietary fiber PI3K/AKT liver metabolomics |
| title | Regulatory Effects of Functional Soluble Dietary Fiber from <i>Saccharina japonica</i> Byproduct on the Liver of Obese Mice with Type 2 Diabetes Mellitus |
| title_full | Regulatory Effects of Functional Soluble Dietary Fiber from <i>Saccharina japonica</i> Byproduct on the Liver of Obese Mice with Type 2 Diabetes Mellitus |
| title_fullStr | Regulatory Effects of Functional Soluble Dietary Fiber from <i>Saccharina japonica</i> Byproduct on the Liver of Obese Mice with Type 2 Diabetes Mellitus |
| title_full_unstemmed | Regulatory Effects of Functional Soluble Dietary Fiber from <i>Saccharina japonica</i> Byproduct on the Liver of Obese Mice with Type 2 Diabetes Mellitus |
| title_short | Regulatory Effects of Functional Soluble Dietary Fiber from <i>Saccharina japonica</i> Byproduct on the Liver of Obese Mice with Type 2 Diabetes Mellitus |
| title_sort | regulatory effects of functional soluble dietary fiber from i saccharina japonica i byproduct on the liver of obese mice with type 2 diabetes mellitus |
| topic | <i>Saccharina japonica</i> dietary fiber PI3K/AKT liver metabolomics |
| url | https://www.mdpi.com/1660-3397/20/2/91 |
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