Chemical composition, antidiabetic, anti-inflammatory, antioxidant and toxicityactivities, of the essential oil of <em>Fortunella margarita</em> peels
The purpose of this study was to identify the principal components of the essential oil extracted from Fortunella margarita peels via hydrodistillation and to evaluate in vitro its anti-diabetic, anti-inflammatory, antioxidant, and toxicity properties. Among the detected compounds were limonene, D-...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2022-10-01
|
| Series: | Journal of Biological Research |
| Subjects: | |
| Online Access: | https://www.pagepressjournals.org/index.php/jbr/article/view/10641 |
| _version_ | 1797986274012823552 |
|---|---|
| author | Zineb Lakache Hinda Hacib Hamza Aliboudhar Mohamed Toumi Mohamed Mahdid Nassima Lamrani Hassina Tounssi Abdelkrim Kameli |
| author_facet | Zineb Lakache Hinda Hacib Hamza Aliboudhar Mohamed Toumi Mohamed Mahdid Nassima Lamrani Hassina Tounssi Abdelkrim Kameli |
| author_sort | Zineb Lakache |
| collection | DOAJ |
| description |
The purpose of this study was to identify the principal components of the essential oil extracted from Fortunella margarita peels via hydrodistillation and to evaluate in vitro its anti-diabetic, anti-inflammatory, antioxidant, and toxicity properties. Among the detected compounds were limonene, D-germacrene, β-myrcene, and α-pinene. Method of inhibiting the denaturation of Bovine Serum Albumin (BSA) was utilized to assess the anti-inflammatory properties of Fortunella margarita. At a concentration of 400g/mL, a high anti-inflammatory effect was observed. The percentage of BSA protection against heat increased with increasing concentration. Also, the evaluation of antidiabetic activity by glucose uptake by yeast cells revealed that Fortunella margarita was more effective than the standard drug novoformine in the presence of 5 mM glucose. The antioxidant potential of the essential oil was evaluated using the DPPH free radical scavenging, reducing power and β-carotene/linoleic acid tests, where the essential oil had much lower antioxidant activity. A bioassay on the lethality of brine shrimp was conducted to determine the toxicity of the essential oil. The study reveals that the essential oil is a possible source of important bioactive compounds and that its constituents may exhibit synergistic effects. Our findings suggest that the essential oil from Fortunella margarita could be used in the future as a substitute for synthetic anti-diabetic, anti-inflammatory, and antioxidant agents with potential applications in the food and pharmaceutical industries.
|
| first_indexed | 2024-04-11T07:31:04Z |
| format | Article |
| id | doaj.art-cd0fe0480cf54a48a049d8e744b0cbe1 |
| institution | Directory Open Access Journal |
| issn | 1826-8838 2284-0230 |
| language | English |
| last_indexed | 2024-04-11T07:31:04Z |
| publishDate | 2022-10-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Journal of Biological Research |
| spelling | doaj.art-cd0fe0480cf54a48a049d8e744b0cbe12022-12-22T04:36:53ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302022-10-0110.4081/jbr.2022.10641Chemical composition, antidiabetic, anti-inflammatory, antioxidant and toxicityactivities, of the essential oil of <em>Fortunella margarita</em> peelsZineb Lakache0Hinda Hacib1Hamza Aliboudhar2Mohamed Toumi3Mohamed Mahdid4Nassima Lamrani5Hassina Tounssi6Abdelkrim Kameli7Laboratory of Ethnobotany and Natural Substances, ENS-Kouba, AlgiersLaboratory of Ethnobotany and Natural Substances, ENS-Kouba, AlgiersUSTHB, Laboratory of Functional Organic Analysis, Faculty of Chemistry, University of Sciences and Technology Houari Boumediene, El Bab-Ezzouar, AlgiersLaboratory of Ethnobotany and Natural Substances, ENS-Kouba, AlgiersLaboratory of Ethnobotany and Natural Substances, ENS-Kouba, AlgiersLaboratory of Ethnobotany and Natural Substances, ENS-Kouba, AlgiersLaboratory of Ethnobotany and Natural Substances, ENS-Kouba, AlgiersLaboratory of Ethnobotany and Natural Substances, ENS-Kouba, Algiers The purpose of this study was to identify the principal components of the essential oil extracted from Fortunella margarita peels via hydrodistillation and to evaluate in vitro its anti-diabetic, anti-inflammatory, antioxidant, and toxicity properties. Among the detected compounds were limonene, D-germacrene, β-myrcene, and α-pinene. Method of inhibiting the denaturation of Bovine Serum Albumin (BSA) was utilized to assess the anti-inflammatory properties of Fortunella margarita. At a concentration of 400g/mL, a high anti-inflammatory effect was observed. The percentage of BSA protection against heat increased with increasing concentration. Also, the evaluation of antidiabetic activity by glucose uptake by yeast cells revealed that Fortunella margarita was more effective than the standard drug novoformine in the presence of 5 mM glucose. The antioxidant potential of the essential oil was evaluated using the DPPH free radical scavenging, reducing power and β-carotene/linoleic acid tests, where the essential oil had much lower antioxidant activity. A bioassay on the lethality of brine shrimp was conducted to determine the toxicity of the essential oil. The study reveals that the essential oil is a possible source of important bioactive compounds and that its constituents may exhibit synergistic effects. Our findings suggest that the essential oil from Fortunella margarita could be used in the future as a substitute for synthetic anti-diabetic, anti-inflammatory, and antioxidant agents with potential applications in the food and pharmaceutical industries. https://www.pagepressjournals.org/index.php/jbr/article/view/10641Fortunella margaritaessential oilGC-MSbiological activities |
| spellingShingle | Zineb Lakache Hinda Hacib Hamza Aliboudhar Mohamed Toumi Mohamed Mahdid Nassima Lamrani Hassina Tounssi Abdelkrim Kameli Chemical composition, antidiabetic, anti-inflammatory, antioxidant and toxicityactivities, of the essential oil of <em>Fortunella margarita</em> peels Journal of Biological Research Fortunella margarita essential oil GC-MS biological activities |
| title | Chemical composition, antidiabetic, anti-inflammatory, antioxidant and toxicityactivities, of the essential oil of <em>Fortunella margarita</em> peels |
| title_full | Chemical composition, antidiabetic, anti-inflammatory, antioxidant and toxicityactivities, of the essential oil of <em>Fortunella margarita</em> peels |
| title_fullStr | Chemical composition, antidiabetic, anti-inflammatory, antioxidant and toxicityactivities, of the essential oil of <em>Fortunella margarita</em> peels |
| title_full_unstemmed | Chemical composition, antidiabetic, anti-inflammatory, antioxidant and toxicityactivities, of the essential oil of <em>Fortunella margarita</em> peels |
| title_short | Chemical composition, antidiabetic, anti-inflammatory, antioxidant and toxicityactivities, of the essential oil of <em>Fortunella margarita</em> peels |
| title_sort | chemical composition antidiabetic anti inflammatory antioxidant and toxicityactivities of the essential oil of em fortunella margarita em peels |
| topic | Fortunella margarita essential oil GC-MS biological activities |
| url | https://www.pagepressjournals.org/index.php/jbr/article/view/10641 |
| work_keys_str_mv | AT zineblakache chemicalcompositionantidiabeticantiinflammatoryantioxidantandtoxicityactivitiesoftheessentialoilofemfortunellamargaritaempeels AT hindahacib chemicalcompositionantidiabeticantiinflammatoryantioxidantandtoxicityactivitiesoftheessentialoilofemfortunellamargaritaempeels AT hamzaaliboudhar chemicalcompositionantidiabeticantiinflammatoryantioxidantandtoxicityactivitiesoftheessentialoilofemfortunellamargaritaempeels AT mohamedtoumi chemicalcompositionantidiabeticantiinflammatoryantioxidantandtoxicityactivitiesoftheessentialoilofemfortunellamargaritaempeels AT mohamedmahdid chemicalcompositionantidiabeticantiinflammatoryantioxidantandtoxicityactivitiesoftheessentialoilofemfortunellamargaritaempeels AT nassimalamrani chemicalcompositionantidiabeticantiinflammatoryantioxidantandtoxicityactivitiesoftheessentialoilofemfortunellamargaritaempeels AT hassinatounssi chemicalcompositionantidiabeticantiinflammatoryantioxidantandtoxicityactivitiesoftheessentialoilofemfortunellamargaritaempeels AT abdelkrimkameli chemicalcompositionantidiabeticantiinflammatoryantioxidantandtoxicityactivitiesoftheessentialoilofemfortunellamargaritaempeels |