Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle
Bovine leukemia virus (BLV) affects cattle health and productivity worldwide, causing abnormal immune function and immunosuppression. Transfer RNA fragments (tRFs) are known to be involved in inhibition of gene expression and have been associated with stress and immune response, tumor growth, and vi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2018.00236/full |
| _version_ | 1828781439552847872 |
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| author | Tasia M. Taxis Tasia M. Taxis Marcus E. Kehrli Rui D’Orey-Branco Eduardo Casas |
| author_facet | Tasia M. Taxis Tasia M. Taxis Marcus E. Kehrli Rui D’Orey-Branco Eduardo Casas |
| author_sort | Tasia M. Taxis |
| collection | DOAJ |
| description | Bovine leukemia virus (BLV) affects cattle health and productivity worldwide, causing abnormal immune function and immunosuppression. Transfer RNA fragments (tRFs) are known to be involved in inhibition of gene expression and have been associated with stress and immune response, tumor growth, and viral infection. The objective of this study was to identify tRFs associated with antibody response to BLV in Holstein cattle. Sera from 14 animals were collected to establish IgG reactivity to BLV by ELISA. Seven animals were seropositive (positive group) and seven were seronegative (negative group) for BLV exposure. Leukocytes from each animal were collected and tRFs were extracted for sequencing. tRF5GlnCTG, tRF5GlnTTG, and tRF5HisGTG, were significantly different between seropositive and seronegative groups (P < 0.0067). In all cases the positive group had a lower number of normalized sequences for tRFs when compared to the negative group. Result suggests that tRF5s could potentially be used as biomarkers to establish exposure of cattle to BLV. |
| first_indexed | 2024-12-11T17:34:13Z |
| format | Article |
| id | doaj.art-cd116a9da8604a6e9f3d1c0d25c24df6 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-11T17:34:13Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-cd116a9da8604a6e9f3d1c0d25c24df62022-12-22T00:56:44ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-07-01910.3389/fgene.2018.00236384114Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein CattleTasia M. Taxis0Tasia M. Taxis1Marcus E. Kehrli2Rui D’Orey-Branco3Eduardo Casas4National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, United StatesDepartment of Animal Science, Michigan State University, East Lansing, MI, United StatesNational Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, United StatesDepartment of Animal Science, Texas A&M University, Overton, TX, United StatesNational Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, United StatesBovine leukemia virus (BLV) affects cattle health and productivity worldwide, causing abnormal immune function and immunosuppression. Transfer RNA fragments (tRFs) are known to be involved in inhibition of gene expression and have been associated with stress and immune response, tumor growth, and viral infection. The objective of this study was to identify tRFs associated with antibody response to BLV in Holstein cattle. Sera from 14 animals were collected to establish IgG reactivity to BLV by ELISA. Seven animals were seropositive (positive group) and seven were seronegative (negative group) for BLV exposure. Leukocytes from each animal were collected and tRFs were extracted for sequencing. tRF5GlnCTG, tRF5GlnTTG, and tRF5HisGTG, were significantly different between seropositive and seronegative groups (P < 0.0067). In all cases the positive group had a lower number of normalized sequences for tRFs when compared to the negative group. Result suggests that tRF5s could potentially be used as biomarkers to establish exposure of cattle to BLV.https://www.frontiersin.org/article/10.3389/fgene.2018.00236/fullBLVbovinecattleleukemia virussmall non-coding RNAtRNA halves |
| spellingShingle | Tasia M. Taxis Tasia M. Taxis Marcus E. Kehrli Rui D’Orey-Branco Eduardo Casas Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle Frontiers in Genetics BLV bovine cattle leukemia virus small non-coding RNA tRNA halves |
| title | Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle |
| title_full | Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle |
| title_fullStr | Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle |
| title_full_unstemmed | Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle |
| title_short | Association of Transfer RNA Fragments in White Blood Cells With Antibody Response to Bovine Leukemia Virus in Holstein Cattle |
| title_sort | association of transfer rna fragments in white blood cells with antibody response to bovine leukemia virus in holstein cattle |
| topic | BLV bovine cattle leukemia virus small non-coding RNA tRNA halves |
| url | https://www.frontiersin.org/article/10.3389/fgene.2018.00236/full |
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