Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT Activity
The objective of this study was to assess the inhibitory effect of the flavonoid aromadendrin on T cell activity to identify a non-cytotoxic immunosuppressive reagent. Conventional and qualitative PCR, MTT assays, flow cytometry and Western blotting were used to evaluate the effect of aromadendrin o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/19/4590 |
| _version_ | 1797551581220044800 |
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| author | Hyun-Su Lee Gil-Saeng Jeong |
| author_facet | Hyun-Su Lee Gil-Saeng Jeong |
| author_sort | Hyun-Su Lee |
| collection | DOAJ |
| description | The objective of this study was to assess the inhibitory effect of the flavonoid aromadendrin on T cell activity to identify a non-cytotoxic immunosuppressive reagent. Conventional and qualitative PCR, MTT assays, flow cytometry and Western blotting were used to evaluate the effect of aromadendrin on the activity, cell viability and confluency, and proximal signal transduction of activated T cells. Aromadendrin effectively regulated IL-2 and IFNγ production in vitro from activated Jurkat T cells without cytotoxicity. Pre-treatment with aromadendrin also suppressed the expression levels of surface molecules CD69, CD25, and CD40L. Reduced calcium (Ca<sup>2+</sup>) influx in activated T cells pre-treated with aromadendrin was observed. Western blotting revealed that aromadendrin blocked the dephosphorylation of nuclear factor of activated T (NFAT) cells and its nuclear translocation. Involvement of the NFκB and MAPK pathways in the inhibitory effect of aromadendrin was also demonstrated. Results obtained demonstrated the suppressive effect of aromadendrin on T cell activation by Ca<sup>2+</sup> influx regulation through NFAT activity suppression of the activated T cells. |
| first_indexed | 2024-03-10T15:47:05Z |
| format | Article |
| id | doaj.art-cd1255ee53b14ba2aa27ffa4d1657328 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:47:05Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cd1255ee53b14ba2aa27ffa4d16573282023-11-20T16:23:43ZengMDPI AGMolecules1420-30492020-10-012519459010.3390/molecules25194590Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT ActivityHyun-Su Lee0Gil-Saeng Jeong1College of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Daegu 42601, KoreaCollege of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Daegu 42601, KoreaThe objective of this study was to assess the inhibitory effect of the flavonoid aromadendrin on T cell activity to identify a non-cytotoxic immunosuppressive reagent. Conventional and qualitative PCR, MTT assays, flow cytometry and Western blotting were used to evaluate the effect of aromadendrin on the activity, cell viability and confluency, and proximal signal transduction of activated T cells. Aromadendrin effectively regulated IL-2 and IFNγ production in vitro from activated Jurkat T cells without cytotoxicity. Pre-treatment with aromadendrin also suppressed the expression levels of surface molecules CD69, CD25, and CD40L. Reduced calcium (Ca<sup>2+</sup>) influx in activated T cells pre-treated with aromadendrin was observed. Western blotting revealed that aromadendrin blocked the dephosphorylation of nuclear factor of activated T (NFAT) cells and its nuclear translocation. Involvement of the NFκB and MAPK pathways in the inhibitory effect of aromadendrin was also demonstrated. Results obtained demonstrated the suppressive effect of aromadendrin on T cell activation by Ca<sup>2+</sup> influx regulation through NFAT activity suppression of the activated T cells.https://www.mdpi.com/1420-3049/25/19/4590T cellsIL-2calciumNFATMAPK |
| spellingShingle | Hyun-Su Lee Gil-Saeng Jeong Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT Activity Molecules T cells IL-2 calcium NFAT MAPK |
| title | Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT Activity |
| title_full | Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT Activity |
| title_fullStr | Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT Activity |
| title_full_unstemmed | Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT Activity |
| title_short | Aromadendrin Inhibits T Cell Activation via Regulation of Calcium Influx and NFAT Activity |
| title_sort | aromadendrin inhibits t cell activation via regulation of calcium influx and nfat activity |
| topic | T cells IL-2 calcium NFAT MAPK |
| url | https://www.mdpi.com/1420-3049/25/19/4590 |
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