Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, Indonesia
Yellow mosaic disease was reported for the first time in Indonesia in 2008. Its infection on yard long bean caused significant yield loss. Mungbean yellow mosaic India virus (MYMIV), member of genus Begomovirus was identified as the main causal agent. Specific and accurate detection is important fo...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Indonesian |
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Perhimpunan Fitopatologi Indonesia
2023-04-01
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| Series: | Jurnal Fitopatologi Indonesia |
| Subjects: | |
| Online Access: | https://jurnal.ipb.ac.id/index.php/jfiti/article/view/46255 |
| _version_ | 1797852079065137152 |
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| author | Sari Nurulita Kikin Mutaqin Sri Hidayat |
| author_facet | Sari Nurulita Kikin Mutaqin Sri Hidayat |
| author_sort | Sari Nurulita |
| collection | DOAJ |
| description |
Yellow mosaic disease was reported for the first time in Indonesia in 2008. Its infection on yard long bean caused significant yield loss. Mungbean yellow mosaic India virus (MYMIV), member of genus Begomovirus was identified as the main causal agent. Specific and accurate detection is important for disease monitoring as part of disease management strategy. The aim of this study was to construct specific primer pairs for quick and robust detection of MYMIV using polymerase chain reaction method. A pair of primers MY1/MY2 was designed in this study to amplify part of MYMIV coat protein. In silico and in vitro test showed that MY1/MY2 primers specifically amplified MYMIV.
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| first_indexed | 2024-04-09T19:28:11Z |
| format | Article |
| id | doaj.art-cd1522247af1447489b814b89aa8c258 |
| institution | Directory Open Access Journal |
| issn | 0215-7950 2339-2479 |
| language | Indonesian |
| last_indexed | 2024-04-09T19:28:11Z |
| publishDate | 2023-04-01 |
| publisher | Perhimpunan Fitopatologi Indonesia |
| record_format | Article |
| series | Jurnal Fitopatologi Indonesia |
| spelling | doaj.art-cd1522247af1447489b814b89aa8c2582023-04-05T06:20:58ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792023-04-0119210.14692/jfi.19.2.83-88Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, IndonesiaSari Nurulita0Kikin MutaqinSri HidayatDepartemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Yellow mosaic disease was reported for the first time in Indonesia in 2008. Its infection on yard long bean caused significant yield loss. Mungbean yellow mosaic India virus (MYMIV), member of genus Begomovirus was identified as the main causal agent. Specific and accurate detection is important for disease monitoring as part of disease management strategy. The aim of this study was to construct specific primer pairs for quick and robust detection of MYMIV using polymerase chain reaction method. A pair of primers MY1/MY2 was designed in this study to amplify part of MYMIV coat protein. In silico and in vitro test showed that MY1/MY2 primers specifically amplified MYMIV. https://jurnal.ipb.ac.id/index.php/jfiti/article/view/46255begomoviruscoat proteinMYMIVpolymerase chain reaction |
| spellingShingle | Sari Nurulita Kikin Mutaqin Sri Hidayat Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, Indonesia Jurnal Fitopatologi Indonesia begomovirus coat protein MYMIV polymerase chain reaction |
| title | Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, Indonesia |
| title_full | Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, Indonesia |
| title_fullStr | Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, Indonesia |
| title_full_unstemmed | Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, Indonesia |
| title_short | Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, Indonesia |
| title_sort | development of specific detection for mungbean yellow mosaic india virus infecting yard long bean in java indonesia |
| topic | begomovirus coat protein MYMIV polymerase chain reaction |
| url | https://jurnal.ipb.ac.id/index.php/jfiti/article/view/46255 |
| work_keys_str_mv | AT sarinurulita developmentofspecificdetectionformungbeanyellowmosaicindiavirusinfectingyardlongbeaninjavaindonesia AT kikinmutaqin developmentofspecificdetectionformungbeanyellowmosaicindiavirusinfectingyardlongbeaninjavaindonesia AT srihidayat developmentofspecificdetectionformungbeanyellowmosaicindiavirusinfectingyardlongbeaninjavaindonesia |