Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, Brazil
<p>Abstract</p> <p>Background</p> <p>Hepatitis B virus (HBV) can be classified into nine genotypes (A-I) defined by sequence divergence of more than 8% based on the complete genome. This study aims to identify the genotypic distribution of HBV in 40 HBsAg-positive patie...
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| Format: | Article |
| Language: | English |
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BMC
2010-11-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/7/1/315 |
| _version_ | 1818691234212872192 |
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| author | Honda Eduardo R Carrilho Flair J Pinho João Vieira Deusilene S Botelho Lívia Alvarado-Mora Mónica V Santos Alcione O Salcedo Juan M |
| author_facet | Honda Eduardo R Carrilho Flair J Pinho João Vieira Deusilene S Botelho Lívia Alvarado-Mora Mónica V Santos Alcione O Salcedo Juan M |
| author_sort | Honda Eduardo R |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Hepatitis B virus (HBV) can be classified into nine genotypes (A-I) defined by sequence divergence of more than 8% based on the complete genome. This study aims to identify the genotypic distribution of HBV in 40 HBsAg-positive patients from Rondônia, Brazil. A fragment of 1306 bp partially comprising surface and polymerase overlapping genes was amplified by PCR. Amplified DNA was purified and sequenced. Amplified DNA was purified and sequenced on an ABI PRISM<sup>® </sup>377 Automatic Sequencer (Applied Biosystems, Foster City, CA, USA). The obtained sequences were aligned with reference sequences obtained from the GenBank using Clustal X software and then edited with Se-Al software. Phylogenetic analyses were conducted by the Markov Chain Monte Carlo (MCMC) approach using BEAST v.1.5.3.</p> <p>Results</p> <p>The subgenotypes distribution was A1 (37.1%), D3 (22.8%), F2a (20.0%), D4 (17.1%) and D2 (2.8%).</p> <p>Conclusions</p> <p>These results for the first HBV genotypic characterization in Rondônia state are consistent with other studies in Brazil, showing the presence of several HBV genotypes that reflects the mixed origin of the population, involving descendants from Native Americans, Europeans, and Africans.</p> |
| first_indexed | 2024-12-17T12:38:39Z |
| format | Article |
| id | doaj.art-cd161aefdccf4634853976ed41a320c7 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-17T12:38:39Z |
| publishDate | 2010-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-cd161aefdccf4634853976ed41a320c72022-12-21T21:48:09ZengBMCVirology Journal1743-422X2010-11-017131510.1186/1743-422X-7-315Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, BrazilHonda Eduardo RCarrilho Flair JPinho JoãoVieira Deusilene SBotelho LíviaAlvarado-Mora Mónica VSantos Alcione OSalcedo Juan M<p>Abstract</p> <p>Background</p> <p>Hepatitis B virus (HBV) can be classified into nine genotypes (A-I) defined by sequence divergence of more than 8% based on the complete genome. This study aims to identify the genotypic distribution of HBV in 40 HBsAg-positive patients from Rondônia, Brazil. A fragment of 1306 bp partially comprising surface and polymerase overlapping genes was amplified by PCR. Amplified DNA was purified and sequenced. Amplified DNA was purified and sequenced on an ABI PRISM<sup>® </sup>377 Automatic Sequencer (Applied Biosystems, Foster City, CA, USA). The obtained sequences were aligned with reference sequences obtained from the GenBank using Clustal X software and then edited with Se-Al software. Phylogenetic analyses were conducted by the Markov Chain Monte Carlo (MCMC) approach using BEAST v.1.5.3.</p> <p>Results</p> <p>The subgenotypes distribution was A1 (37.1%), D3 (22.8%), F2a (20.0%), D4 (17.1%) and D2 (2.8%).</p> <p>Conclusions</p> <p>These results for the first HBV genotypic characterization in Rondônia state are consistent with other studies in Brazil, showing the presence of several HBV genotypes that reflects the mixed origin of the population, involving descendants from Native Americans, Europeans, and Africans.</p>http://www.virologyj.com/content/7/1/315 |
| spellingShingle | Honda Eduardo R Carrilho Flair J Pinho João Vieira Deusilene S Botelho Lívia Alvarado-Mora Mónica V Santos Alcione O Salcedo Juan M Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, Brazil Virology Journal |
| title | Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, Brazil |
| title_full | Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, Brazil |
| title_fullStr | Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, Brazil |
| title_full_unstemmed | Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, Brazil |
| title_short | Characterization of Hepatitis B virus (HBV) genotypes in patients from Rondônia, Brazil |
| title_sort | characterization of hepatitis b virus hbv genotypes in patients from rondonia brazil |
| url | http://www.virologyj.com/content/7/1/315 |
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