Isolation and characterization of Chlorella sp. mutants with enhanced thermo- and CO2 tolerances for CO2 sequestration and utilization of flue gases
Abstract Background The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temp...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | Biotechnology for Biofuels |
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| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1590-9 |
| _version_ | 1811257526648307712 |
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| author | Hsiang-Hui Chou Hsiang-Yen Su Xiang-Di Song Te-Jin Chow Chun-Yen Chen Jo-Shu Chang Tse-Min Lee |
| author_facet | Hsiang-Hui Chou Hsiang-Yen Su Xiang-Di Song Te-Jin Chow Chun-Yen Chen Jo-Shu Chang Tse-Min Lee |
| author_sort | Hsiang-Hui Chou |
| collection | DOAJ |
| description | Abstract Background The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temperatures is also critical for outdoor microalgal mass cultivation. Therefore, flue gas- and thermo-tolerant mutants of Chlorella vulgaris ESP-31 were generated and characterized for their ability to grow under various conditions. Results In this study, we obtained two CO2- and thermo-tolerant mutants of Chlorella vulgaris ESP-31, namely, 283 and 359, with enhanced CO2 tolerance and thermo-tolerance by using N-methyl-N-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by screening at high temperature and under high CO2 conditions with the w-zipper pouch selection method. The two mutants exhibited higher photosynthetic activity and biomass productivity than that of the ESP-31 wild type. More importantly, the mutants were able to grow at high temperature (40 °C) and a high concentration of simulated flue gas (25% CO2, 80–90 ppm SO2, 90–100 ppm NO) and showed higher carbohydrate and lipid contents than did the ESP-31 wild type. Conclusions The two thermo- and flue gas-tolerant mutants of Chlorella vulgaris ESP-31 were useful for CO2 mitigation from flue gas under heated conditions and for the production of carbohydrates and biodiesel directly using CO2 from flue gas. |
| first_indexed | 2024-04-12T17:57:45Z |
| format | Article |
| id | doaj.art-cd1a1daf19eb4887b765975af13a2d3a |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-12T17:57:45Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-cd1a1daf19eb4887b765975af13a2d3a2022-12-22T03:22:16ZengBMCBiotechnology for Biofuels1754-68342019-10-0112111410.1186/s13068-019-1590-9Isolation and characterization of Chlorella sp. mutants with enhanced thermo- and CO2 tolerances for CO2 sequestration and utilization of flue gasesHsiang-Hui Chou0Hsiang-Yen Su1Xiang-Di Song2Te-Jin Chow3Chun-Yen Chen4Jo-Shu Chang5Tse-Min Lee6Department of Biotechnology, Fooyin UniversityChina-Latin America Joint Laboratory for Clean Energy and Climate Change, School of Chemical Engineering and Energy Technology, Dongguan University of TechnologyDepartment of Biotechnology, Fooyin UniversityDepartment of Biotechnology, Fooyin UniversityUniversity Center of Bioscience and Biotechnology, National Cheng Kung UniversityDepartment of Chemical Engineering, National Cheng-Kung UniversityDepartment of Marine Biotechnology and Resources, National Sun Yat-sen UniversityAbstract Background The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temperatures is also critical for outdoor microalgal mass cultivation. Therefore, flue gas- and thermo-tolerant mutants of Chlorella vulgaris ESP-31 were generated and characterized for their ability to grow under various conditions. Results In this study, we obtained two CO2- and thermo-tolerant mutants of Chlorella vulgaris ESP-31, namely, 283 and 359, with enhanced CO2 tolerance and thermo-tolerance by using N-methyl-N-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by screening at high temperature and under high CO2 conditions with the w-zipper pouch selection method. The two mutants exhibited higher photosynthetic activity and biomass productivity than that of the ESP-31 wild type. More importantly, the mutants were able to grow at high temperature (40 °C) and a high concentration of simulated flue gas (25% CO2, 80–90 ppm SO2, 90–100 ppm NO) and showed higher carbohydrate and lipid contents than did the ESP-31 wild type. Conclusions The two thermo- and flue gas-tolerant mutants of Chlorella vulgaris ESP-31 were useful for CO2 mitigation from flue gas under heated conditions and for the production of carbohydrates and biodiesel directly using CO2 from flue gas.http://link.springer.com/article/10.1186/s13068-019-1590-9Flue gasMicroalgaeChlorella sp.Thermo-tolerance |
| spellingShingle | Hsiang-Hui Chou Hsiang-Yen Su Xiang-Di Song Te-Jin Chow Chun-Yen Chen Jo-Shu Chang Tse-Min Lee Isolation and characterization of Chlorella sp. mutants with enhanced thermo- and CO2 tolerances for CO2 sequestration and utilization of flue gases Biotechnology for Biofuels Flue gas Microalgae Chlorella sp. Thermo-tolerance |
| title | Isolation and characterization of Chlorella sp. mutants with enhanced thermo- and CO2 tolerances for CO2 sequestration and utilization of flue gases |
| title_full | Isolation and characterization of Chlorella sp. mutants with enhanced thermo- and CO2 tolerances for CO2 sequestration and utilization of flue gases |
| title_fullStr | Isolation and characterization of Chlorella sp. mutants with enhanced thermo- and CO2 tolerances for CO2 sequestration and utilization of flue gases |
| title_full_unstemmed | Isolation and characterization of Chlorella sp. mutants with enhanced thermo- and CO2 tolerances for CO2 sequestration and utilization of flue gases |
| title_short | Isolation and characterization of Chlorella sp. mutants with enhanced thermo- and CO2 tolerances for CO2 sequestration and utilization of flue gases |
| title_sort | isolation and characterization of chlorella sp mutants with enhanced thermo and co2 tolerances for co2 sequestration and utilization of flue gases |
| topic | Flue gas Microalgae Chlorella sp. Thermo-tolerance |
| url | http://link.springer.com/article/10.1186/s13068-019-1590-9 |
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