Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application
In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vi...
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| Language: | English |
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/4/2191 |
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| author | Jing Huang Nairveen Ali Elsie Quansah Shuxia Guo Michel Noutsias Tobias Meyer-Zedler Thomas Bocklitz Jürgen Popp Ute Neugebauer Anuradha Ramoji |
| author_facet | Jing Huang Nairveen Ali Elsie Quansah Shuxia Guo Michel Noutsias Tobias Meyer-Zedler Thomas Bocklitz Jürgen Popp Ute Neugebauer Anuradha Ramoji |
| author_sort | Jing Huang |
| collection | DOAJ |
| description | In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational signal. However, most often encountered challenge is the inhomogeneous redistribution of biomolecules due to the coffee-ring effect. In this study, the variation in biomolecule distribution within the dried-sample droplet has been investigated using Raman and FT-IR spectroscopy and fluorescence lifetime imaging method. The plasma-sample from healthy donors were investigated to show the spectral differences between the inner and outer-ring region of the dried-sample droplet. Further, the preferred location of deposition of the most abundant protein albumin in the blood during the drying process of the plasma has been illustrated by using deuterated albumin. Subsequently, two patients with different cardiac-related diseases were investigated exemplarily to illustrate the variation in the pattern of plasma and serum biomolecule distribution during the drying process and its impact on patient-stratification. The study shows that a uniform sampling position of the droplet, both at the inner and the outer ring, is necessary for thorough clinical characterization of the patient’s plasma and serum sample using vibrational spectroscopy. |
| first_indexed | 2024-03-09T00:37:50Z |
| format | Article |
| id | doaj.art-cd1a6f3321274993ae299f0e3c3654bc |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T00:37:50Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cd1a6f3321274993ae299f0e3c3654bc2023-12-11T18:02:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224219110.3390/ijms22042191Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical ApplicationJing Huang0Nairveen Ali1Elsie Quansah2Shuxia Guo3Michel Noutsias4Tobias Meyer-Zedler5Thomas Bocklitz6Jürgen Popp7Ute Neugebauer8Anuradha Ramoji9Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, GermanyInstitute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, GermanyInstitute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, GermanyInstitute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, GermanyDepartment of Cardiology Internal Medicine, Jena University Hospital, Am Klinikum 1, D-07747 Jena, GermanyInstitute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, GermanyInstitute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, GermanyInstitute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, GermanyInstitute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, GermanyInstitute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, D-07743 Jena, GermanyIn recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational signal. However, most often encountered challenge is the inhomogeneous redistribution of biomolecules due to the coffee-ring effect. In this study, the variation in biomolecule distribution within the dried-sample droplet has been investigated using Raman and FT-IR spectroscopy and fluorescence lifetime imaging method. The plasma-sample from healthy donors were investigated to show the spectral differences between the inner and outer-ring region of the dried-sample droplet. Further, the preferred location of deposition of the most abundant protein albumin in the blood during the drying process of the plasma has been illustrated by using deuterated albumin. Subsequently, two patients with different cardiac-related diseases were investigated exemplarily to illustrate the variation in the pattern of plasma and serum biomolecule distribution during the drying process and its impact on patient-stratification. The study shows that a uniform sampling position of the droplet, both at the inner and the outer ring, is necessary for thorough clinical characterization of the patient’s plasma and serum sample using vibrational spectroscopy.https://www.mdpi.com/1422-0067/22/4/2191plasmaserumcoffee-ring effectcardiac patientsvibrational spectroscopyfluorescence lifetime |
| spellingShingle | Jing Huang Nairveen Ali Elsie Quansah Shuxia Guo Michel Noutsias Tobias Meyer-Zedler Thomas Bocklitz Jürgen Popp Ute Neugebauer Anuradha Ramoji Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application International Journal of Molecular Sciences plasma serum coffee-ring effect cardiac patients vibrational spectroscopy fluorescence lifetime |
| title | Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application |
| title_full | Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application |
| title_fullStr | Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application |
| title_full_unstemmed | Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application |
| title_short | Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application |
| title_sort | vibrational spectroscopic investigation of blood plasma and serum by drop coating deposition for clinical application |
| topic | plasma serum coffee-ring effect cardiac patients vibrational spectroscopy fluorescence lifetime |
| url | https://www.mdpi.com/1422-0067/22/4/2191 |
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