The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis.
As the number of contact lens users increases, contact lens induced corneal infection is becoming more common. Acanthamoeba keratitis (AK) is a type of those which is caused by Acanthamoeba species, and may cause severe ocular inflammation and visual loss. We evaluated whether Torreya nucifera (T. n...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0281141 |
| _version_ | 1811167203760799744 |
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| author | Min Seung Kang Sangyoon Kim Da Som Kim Hak Sun Yu Ji Eun Lee |
| author_facet | Min Seung Kang Sangyoon Kim Da Som Kim Hak Sun Yu Ji Eun Lee |
| author_sort | Min Seung Kang |
| collection | DOAJ |
| description | As the number of contact lens users increases, contact lens induced corneal infection is becoming more common. Acanthamoeba keratitis (AK) is a type of those which is caused by Acanthamoeba species, and may cause severe ocular inflammation and visual loss. We evaluated whether Torreya nucifera (T. nucifera) extract has an anti-amoebic effect and studied its mechanism of action on Acanthamoeba lugdunensis (A. lugdunensis). Cell viability was tested using the alamarBlue™ method, and the cell death mechanism was confirmed using the Tali® Apoptosis Kit. The SYTOX® Green assay was performed to check the plasma membrane permeability. The JC-1 dye was used to measure the mitochondrial membrane potential. A CellTiter-Glo® Luminescent Assay was used to measure the adenosine-triphosphate (ATP) level. Morphological changes in the mitochondria were examined by transmission electron microscopy (TEM). Cystic changes and a decrease in cell viability after treatment with T. nucifera were observed. Both apoptotic and necrotic cells were found in the Tali® Apoptosis assay. There was no significant difference in plasma membrane permeability between the control and T. nucifera treated groups. The collapse of the mitochondrial membrane potential and reduced ATP level in A. lugdunensis was confirmed in the groups treated with T. nucifera. Structural damage to the mitochondria was observed on TEM in the groups treated with T. nucifera. T. nucifera showed an anti-amoebic effect on A. lugdunensis, by inducing the loss of mitochondrial membrane potential. Thus, it could be a future therapeutic agent for AK. |
| first_indexed | 2024-04-10T16:06:24Z |
| format | Article |
| id | doaj.art-cd1b23ee4c234c50b2067d096f1b573c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T16:06:24Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cd1b23ee4c234c50b2067d096f1b573c2023-02-10T05:31:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01182e028114110.1371/journal.pone.0281141The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis.Min Seung KangSangyoon KimDa Som KimHak Sun YuJi Eun LeeAs the number of contact lens users increases, contact lens induced corneal infection is becoming more common. Acanthamoeba keratitis (AK) is a type of those which is caused by Acanthamoeba species, and may cause severe ocular inflammation and visual loss. We evaluated whether Torreya nucifera (T. nucifera) extract has an anti-amoebic effect and studied its mechanism of action on Acanthamoeba lugdunensis (A. lugdunensis). Cell viability was tested using the alamarBlue™ method, and the cell death mechanism was confirmed using the Tali® Apoptosis Kit. The SYTOX® Green assay was performed to check the plasma membrane permeability. The JC-1 dye was used to measure the mitochondrial membrane potential. A CellTiter-Glo® Luminescent Assay was used to measure the adenosine-triphosphate (ATP) level. Morphological changes in the mitochondria were examined by transmission electron microscopy (TEM). Cystic changes and a decrease in cell viability after treatment with T. nucifera were observed. Both apoptotic and necrotic cells were found in the Tali® Apoptosis assay. There was no significant difference in plasma membrane permeability between the control and T. nucifera treated groups. The collapse of the mitochondrial membrane potential and reduced ATP level in A. lugdunensis was confirmed in the groups treated with T. nucifera. Structural damage to the mitochondria was observed on TEM in the groups treated with T. nucifera. T. nucifera showed an anti-amoebic effect on A. lugdunensis, by inducing the loss of mitochondrial membrane potential. Thus, it could be a future therapeutic agent for AK.https://doi.org/10.1371/journal.pone.0281141 |
| spellingShingle | Min Seung Kang Sangyoon Kim Da Som Kim Hak Sun Yu Ji Eun Lee The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis. PLoS ONE |
| title | The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis. |
| title_full | The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis. |
| title_fullStr | The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis. |
| title_full_unstemmed | The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis. |
| title_short | The amoebicidal effect of Torreya nucifera extract on Acanthamoeba lugdunensis. |
| title_sort | amoebicidal effect of torreya nucifera extract on acanthamoeba lugdunensis |
| url | https://doi.org/10.1371/journal.pone.0281141 |
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