The AlkB Homolog SlALKBH10B Negatively Affects Drought and Salt Tolerance in <i>Solanum lycopersicum</i>
ALKBH proteins, the homologs of <i>Escherichia coli</i> AlkB dioxygenase, constitute a single-protein repair system that safeguards cellular DNA and RNA against the harmful effects of alkylating agents. ALKBH10B, the first discovered <i>N</i><sup>6</sup>-methylade...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/173 |
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| author | Hui Shen Ying Zhou Changguang Liao Qiaoli Xie Guoping Chen Zongli Hu Ting Wu |
| author_facet | Hui Shen Ying Zhou Changguang Liao Qiaoli Xie Guoping Chen Zongli Hu Ting Wu |
| author_sort | Hui Shen |
| collection | DOAJ |
| description | ALKBH proteins, the homologs of <i>Escherichia coli</i> AlkB dioxygenase, constitute a single-protein repair system that safeguards cellular DNA and RNA against the harmful effects of alkylating agents. ALKBH10B, the first discovered <i>N</i><sup>6</sup>-methyladenosine (m<sup>6</sup>A) demethylase in Arabidopsis (<i>Arabidopsis thaliana</i>), has been shown to regulate plant growth, development, and stress responses. However, until now, the functional role of the plant ALKBH10B has solely been reported in arabidopsis, cotton, and poplar, leaving its functional implications in other plant species shrouded in mystery. In this study, we identified the AlkB homolog SlALKBH10B in tomato (<i>Solanum lycopersicum</i>) through phylogenetic and gene expression analyses. <i>SlALKBH10B</i> exhibited a wide range of expression patterns and was induced by exogenous abscisic acid (ABA) and abiotic stresses. By employing CRISPR/Cas9 gene editing techniques to knock out <i>SlALKBH10B</i>, we observed an increased sensitivity of mutants to ABA treatment and upregulation of gene expression related to ABA synthesis and response. Furthermore, the <i>Slalkbh10b</i> mutants displayed an enhanced tolerance to drought and salt stress, characterized by higher water retention, accumulation of photosynthetic products, proline accumulation, and lower levels of reactive oxygen species and cellular damage. Collectively, these findings provide insights into the negative impact of <i>SlALKBH10B</i> on drought and salt tolerance in tomato plant, expanding our understanding of the biological functionality of <i>SlALKBH10B</i>. |
| first_indexed | 2024-03-08T15:06:45Z |
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| language | English |
| last_indexed | 2024-03-08T15:06:45Z |
| publishDate | 2023-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cd1ce437b4ba4fea8f93f511681588212024-01-10T14:58:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125117310.3390/ijms25010173The AlkB Homolog SlALKBH10B Negatively Affects Drought and Salt Tolerance in <i>Solanum lycopersicum</i>Hui Shen0Ying Zhou1Changguang Liao2Qiaoli Xie3Guoping Chen4Zongli Hu5Ting Wu6Laboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400030, ChinaLaboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400030, ChinaLaboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400030, ChinaLaboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400030, ChinaLaboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400030, ChinaLaboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400030, ChinaLaboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400030, ChinaALKBH proteins, the homologs of <i>Escherichia coli</i> AlkB dioxygenase, constitute a single-protein repair system that safeguards cellular DNA and RNA against the harmful effects of alkylating agents. ALKBH10B, the first discovered <i>N</i><sup>6</sup>-methyladenosine (m<sup>6</sup>A) demethylase in Arabidopsis (<i>Arabidopsis thaliana</i>), has been shown to regulate plant growth, development, and stress responses. However, until now, the functional role of the plant ALKBH10B has solely been reported in arabidopsis, cotton, and poplar, leaving its functional implications in other plant species shrouded in mystery. In this study, we identified the AlkB homolog SlALKBH10B in tomato (<i>Solanum lycopersicum</i>) through phylogenetic and gene expression analyses. <i>SlALKBH10B</i> exhibited a wide range of expression patterns and was induced by exogenous abscisic acid (ABA) and abiotic stresses. By employing CRISPR/Cas9 gene editing techniques to knock out <i>SlALKBH10B</i>, we observed an increased sensitivity of mutants to ABA treatment and upregulation of gene expression related to ABA synthesis and response. Furthermore, the <i>Slalkbh10b</i> mutants displayed an enhanced tolerance to drought and salt stress, characterized by higher water retention, accumulation of photosynthetic products, proline accumulation, and lower levels of reactive oxygen species and cellular damage. Collectively, these findings provide insights into the negative impact of <i>SlALKBH10B</i> on drought and salt tolerance in tomato plant, expanding our understanding of the biological functionality of <i>SlALKBH10B</i>.https://www.mdpi.com/1422-0067/25/1/173AlkB<i>SlALKBH10B</i>abscisic aciddroughtsalttomato |
| spellingShingle | Hui Shen Ying Zhou Changguang Liao Qiaoli Xie Guoping Chen Zongli Hu Ting Wu The AlkB Homolog SlALKBH10B Negatively Affects Drought and Salt Tolerance in <i>Solanum lycopersicum</i> International Journal of Molecular Sciences AlkB <i>SlALKBH10B</i> abscisic acid drought salt tomato |
| title | The AlkB Homolog SlALKBH10B Negatively Affects Drought and Salt Tolerance in <i>Solanum lycopersicum</i> |
| title_full | The AlkB Homolog SlALKBH10B Negatively Affects Drought and Salt Tolerance in <i>Solanum lycopersicum</i> |
| title_fullStr | The AlkB Homolog SlALKBH10B Negatively Affects Drought and Salt Tolerance in <i>Solanum lycopersicum</i> |
| title_full_unstemmed | The AlkB Homolog SlALKBH10B Negatively Affects Drought and Salt Tolerance in <i>Solanum lycopersicum</i> |
| title_short | The AlkB Homolog SlALKBH10B Negatively Affects Drought and Salt Tolerance in <i>Solanum lycopersicum</i> |
| title_sort | alkb homolog slalkbh10b negatively affects drought and salt tolerance in i solanum lycopersicum i |
| topic | AlkB <i>SlALKBH10B</i> abscisic acid drought salt tomato |
| url | https://www.mdpi.com/1422-0067/25/1/173 |
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