The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (<i>Rangifer tarandus</i> L.) from the Russian Arctic Zone: Regional and Seasonal Effects
The reindeer (<i>Rangifer tarandus</i> L.) is a unique animal inhabitant of arctic regions. Low ambient temperatures and scant diets (primarily, lichens) have resulted in different evolutional adaptations, including the composition of the ruminal microbiota. In the study presented here,...
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MDPI AG
2021-03-01
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| author | Larisa A. Ilina Valentina A. Filippova Evgeni A. Brazhnik Andrey V. Dubrovin Elena A. Yildirim Timur P. Dunyashev Georgiy Y. Laptev Natalia I. Novikova Dmitriy V. Sobolev Aleksandr A. Yuzhakov Kasim A. Laishev |
| author_facet | Larisa A. Ilina Valentina A. Filippova Evgeni A. Brazhnik Andrey V. Dubrovin Elena A. Yildirim Timur P. Dunyashev Georgiy Y. Laptev Natalia I. Novikova Dmitriy V. Sobolev Aleksandr A. Yuzhakov Kasim A. Laishev |
| author_sort | Larisa A. Ilina |
| collection | DOAJ |
| description | The reindeer (<i>Rangifer tarandus</i> L.) is a unique animal inhabitant of arctic regions. Low ambient temperatures and scant diets (primarily, lichens) have resulted in different evolutional adaptations, including the composition of the ruminal microbiota. In the study presented here, the effects of seasonal and regional aspects of the composition of the ruminal microbiota in reindeer (Nenets breed, 38 animals) were studied (wooded tundra from the Yamalo-Nenetski Autonomous District (YNAD) vs. from the Nenetski Autonomous District (NAD)). The ruminal content of calves (<i>n</i> = 12) and adult animals (<i>n</i> = 26, 15 males and 11 females) was sampled in the summer (<i>n</i> = 16) and winter seasons (<i>n</i> = 22). The composition of the ruminal microbial population was determined by the V3–V4 16S rRNA gene region sequencing. It was found that the population was dominated by Bacteroidetes and Firmicutes phyla, followed by <i>Spirochaetes</i> and <i>Verrucomicrobia</i>. An analysis of the community using non-metric multidimensional scaling and Bray–Curtis similarity metrics provided evidence that the most influential factors affecting the composition of ruminal microbiota are the region (<i>p</i> = 0.001) and season (<i>p</i> = 0.001); heat map analysis revealed several communities that are strongly affected by these two factors. In the summer season, the following communities were significantly larger compared to in the winter season: <i>Coriobactriaceae</i>, <i>Erysipelothrihaceae</i>, and <i>Mycoplasmataceae</i>. The following communities were significantly larger in the winter season compared to in summer: <i>Paraprevotellaceae</i>, <i>Butyrivibrio</i> spp., <i>Succiniclasticum</i> spp., <i>Coprococcus</i> spp., <i>Ruminococcus</i> spp., and <i>Pseudobutyrivibrio</i> spp. In NAD (tundra), the following communities were significantly larger in comparison to YNAD (wooded tundra): <i>Verrucomicrobia</i> (Verruco-5), <i>Anaerolinaceae</i>, PeHg47 <i>Planctomycetes</i>, cellulolytic <i>Lachnospiraceae</i>, and <i>Succiniclasticum</i> spp. The following bacterial groups were significantly larger in YNAD in comparison to NAD: cellulolytic <i>Ruminococaceae</i>, <i>Dehalobacteriaceae</i>, <i>Veillionelaceae</i>, and <i>Oscilospira</i> spp. The significant differences in the ruminal microbial population were primarily related to the ingredients of diets, affected by region and season. The summer-related increases in the communities of certain pathogens (<i>Mycoplasmataceae</i>, <i>Fusobacterium</i> spp., <i>Porphyromonas endodentalis</i>) were found. Regional differences were primarily related to the ratio of the species involved in ruminal cellulose degradation and ruminal fatty acids metabolism; these differences reflect the regional dissimilarities in botanical diet ingredients. |
| first_indexed | 2024-03-10T13:00:22Z |
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| id | doaj.art-cd1d1522c48c49768a44096e6f0a9a06 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:00:22Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj.art-cd1d1522c48c49768a44096e6f0a9a062023-11-21T11:34:43ZengMDPI AGAnimals2076-26152021-03-0111391110.3390/ani11030911The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (<i>Rangifer tarandus</i> L.) from the Russian Arctic Zone: Regional and Seasonal EffectsLarisa A. Ilina0Valentina A. Filippova1Evgeni A. Brazhnik2Andrey V. Dubrovin3Elena A. Yildirim4Timur P. Dunyashev5Georgiy Y. Laptev6Natalia I. Novikova7Dmitriy V. Sobolev8Aleksandr A. Yuzhakov9Kasim A. Laishev10BIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, RussiaBIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, RussiaBIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, RussiaBIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, RussiaBIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, RussiaBIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, RussiaBIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, RussiaBIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, RussiaBIOTROF + Ltd., 8 Malinovskaya St, Liter A, 7-N, Pushkin, 196602 St. Petersburg, RussiaDepartment of Animal Husbandry and Environmental Management of the Arctic, Federal Research Center of Russian Academy Sciences, 7, Sh. Podbel’skogo, Pushkin, 196608 St. Petersburg, RussiaDepartment of Animal Husbandry and Environmental Management of the Arctic, Federal Research Center of Russian Academy Sciences, 7, Sh. Podbel’skogo, Pushkin, 196608 St. Petersburg, RussiaThe reindeer (<i>Rangifer tarandus</i> L.) is a unique animal inhabitant of arctic regions. Low ambient temperatures and scant diets (primarily, lichens) have resulted in different evolutional adaptations, including the composition of the ruminal microbiota. In the study presented here, the effects of seasonal and regional aspects of the composition of the ruminal microbiota in reindeer (Nenets breed, 38 animals) were studied (wooded tundra from the Yamalo-Nenetski Autonomous District (YNAD) vs. from the Nenetski Autonomous District (NAD)). The ruminal content of calves (<i>n</i> = 12) and adult animals (<i>n</i> = 26, 15 males and 11 females) was sampled in the summer (<i>n</i> = 16) and winter seasons (<i>n</i> = 22). The composition of the ruminal microbial population was determined by the V3–V4 16S rRNA gene region sequencing. It was found that the population was dominated by Bacteroidetes and Firmicutes phyla, followed by <i>Spirochaetes</i> and <i>Verrucomicrobia</i>. An analysis of the community using non-metric multidimensional scaling and Bray–Curtis similarity metrics provided evidence that the most influential factors affecting the composition of ruminal microbiota are the region (<i>p</i> = 0.001) and season (<i>p</i> = 0.001); heat map analysis revealed several communities that are strongly affected by these two factors. In the summer season, the following communities were significantly larger compared to in the winter season: <i>Coriobactriaceae</i>, <i>Erysipelothrihaceae</i>, and <i>Mycoplasmataceae</i>. The following communities were significantly larger in the winter season compared to in summer: <i>Paraprevotellaceae</i>, <i>Butyrivibrio</i> spp., <i>Succiniclasticum</i> spp., <i>Coprococcus</i> spp., <i>Ruminococcus</i> spp., and <i>Pseudobutyrivibrio</i> spp. In NAD (tundra), the following communities were significantly larger in comparison to YNAD (wooded tundra): <i>Verrucomicrobia</i> (Verruco-5), <i>Anaerolinaceae</i>, PeHg47 <i>Planctomycetes</i>, cellulolytic <i>Lachnospiraceae</i>, and <i>Succiniclasticum</i> spp. The following bacterial groups were significantly larger in YNAD in comparison to NAD: cellulolytic <i>Ruminococaceae</i>, <i>Dehalobacteriaceae</i>, <i>Veillionelaceae</i>, and <i>Oscilospira</i> spp. The significant differences in the ruminal microbial population were primarily related to the ingredients of diets, affected by region and season. The summer-related increases in the communities of certain pathogens (<i>Mycoplasmataceae</i>, <i>Fusobacterium</i> spp., <i>Porphyromonas endodentalis</i>) were found. Regional differences were primarily related to the ratio of the species involved in ruminal cellulose degradation and ruminal fatty acids metabolism; these differences reflect the regional dissimilarities in botanical diet ingredients.https://www.mdpi.com/2076-2615/11/3/911reindeerrumenmicrobiomeNGSarctic zone |
| spellingShingle | Larisa A. Ilina Valentina A. Filippova Evgeni A. Brazhnik Andrey V. Dubrovin Elena A. Yildirim Timur P. Dunyashev Georgiy Y. Laptev Natalia I. Novikova Dmitriy V. Sobolev Aleksandr A. Yuzhakov Kasim A. Laishev The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (<i>Rangifer tarandus</i> L.) from the Russian Arctic Zone: Regional and Seasonal Effects Animals reindeer rumen microbiome NGS arctic zone |
| title | The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (<i>Rangifer tarandus</i> L.) from the Russian Arctic Zone: Regional and Seasonal Effects |
| title_full | The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (<i>Rangifer tarandus</i> L.) from the Russian Arctic Zone: Regional and Seasonal Effects |
| title_fullStr | The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (<i>Rangifer tarandus</i> L.) from the Russian Arctic Zone: Regional and Seasonal Effects |
| title_full_unstemmed | The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (<i>Rangifer tarandus</i> L.) from the Russian Arctic Zone: Regional and Seasonal Effects |
| title_short | The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (<i>Rangifer tarandus</i> L.) from the Russian Arctic Zone: Regional and Seasonal Effects |
| title_sort | comparative analysis of the ruminal bacterial population in reindeer i rangifer tarandus i l from the russian arctic zone regional and seasonal effects |
| topic | reindeer rumen microbiome NGS arctic zone |
| url | https://www.mdpi.com/2076-2615/11/3/911 |
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