Detection of Oxacillin/Cefoxitin Resistance in <i>Staphylococcus aureus</i> Present in Recurrent Tonsillitis
Background: Recurrent tonsillitis is one of the most common diseases in childhood, caused many times by ß-lactam-resistant <i>S. aureus</i>. The objective of this study was to investigate an alternative method to identify resistance to oxacillin/cefoxitin in <i>S. aureus</i>...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/615 |
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| author | Aline Cristine Magalhães Costa Messias Aline Rodrigues Gama Letícia Suriano de Almeida Prado Paulo Alex Neves da Silva Arlindo Rodrigues Galvão Filho Clarimar José Coelho Melissa Ameloti Gomes Avelino José Daniel Gonçalves Vieira Lilian Carla Carneiro |
| author_facet | Aline Cristine Magalhães Costa Messias Aline Rodrigues Gama Letícia Suriano de Almeida Prado Paulo Alex Neves da Silva Arlindo Rodrigues Galvão Filho Clarimar José Coelho Melissa Ameloti Gomes Avelino José Daniel Gonçalves Vieira Lilian Carla Carneiro |
| author_sort | Aline Cristine Magalhães Costa Messias |
| collection | DOAJ |
| description | Background: Recurrent tonsillitis is one of the most common diseases in childhood, caused many times by ß-lactam-resistant <i>S. aureus</i>. The objective of this study was to investigate an alternative method to identify resistance to oxacillin/cefoxitin in <i>S. aureus</i> from hospitalized children with recurrent tonsillitis. Methods: The samples of <i>S. aureus</i> came from patients with recurrent tonsillitis and were used in 16S rRNA sequencing and an antibiogram test for identification and verifying resistance, after which HSI methodology were applied for separation of <i>S. aureus</i> resistances. Results: The <i>S. aureus</i> isolated showed sensitivity to oxacillin/cefoxitin and the diagnostic images show a visual description of the resistance different groups formed, that may be related to sensitivity and resistance to oxacillin/cefoxitin, characterizing the MRSA <i>S. aureus</i>. Conclusions: Samples that showed phenotypic resistance to oxacillin/cefoxitin were clearly separated from samples that did not show this resistance. A PLS-DA model predicted the presence of resistance to oxacillin/cefoxitin in <i>S. aureus</i> samples and it was possible to observe the pixels classified as MRSA. The HSI was able to successfully discriminate samples in replicas that were sensitive and resistant, based on the calibration model it received. |
| first_indexed | 2024-03-09T11:00:34Z |
| format | Article |
| id | doaj.art-cd1ec19d2f524468a36a453be2517c2f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:00:34Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cd1ec19d2f524468a36a453be2517c2f2023-12-01T01:21:33ZengMDPI AGMicroorganisms2076-26072023-02-0111361510.3390/microorganisms11030615Detection of Oxacillin/Cefoxitin Resistance in <i>Staphylococcus aureus</i> Present in Recurrent TonsillitisAline Cristine Magalhães Costa Messias0Aline Rodrigues Gama1Letícia Suriano de Almeida Prado2Paulo Alex Neves da Silva3Arlindo Rodrigues Galvão Filho4Clarimar José Coelho5Melissa Ameloti Gomes Avelino6José Daniel Gonçalves Vieira7Lilian Carla Carneiro8Institute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-020, GO, BrazilInstitute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-020, GO, BrazilFaculty of Medicine, Federal University of Goiás, 235 Street, Goiânia 74690-900, GO, BrazilFaculty of Medicine, Federal University of Goiás, 235 Street, Goiânia 74690-900, GO, BrazilEngineering School, Pontifical Catholic University of Goiás, Avenue Universitária, Goiânia 74175-120, GO, BrazilEngineering School, Pontifical Catholic University of Goiás, Avenue Universitária, Goiânia 74175-120, GO, BrazilFaculty of Medicine, Federal University of Goiás, 235 Street, Goiânia 74690-900, GO, BrazilInstitute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-020, GO, BrazilInstitute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-020, GO, BrazilBackground: Recurrent tonsillitis is one of the most common diseases in childhood, caused many times by ß-lactam-resistant <i>S. aureus</i>. The objective of this study was to investigate an alternative method to identify resistance to oxacillin/cefoxitin in <i>S. aureus</i> from hospitalized children with recurrent tonsillitis. Methods: The samples of <i>S. aureus</i> came from patients with recurrent tonsillitis and were used in 16S rRNA sequencing and an antibiogram test for identification and verifying resistance, after which HSI methodology were applied for separation of <i>S. aureus</i> resistances. Results: The <i>S. aureus</i> isolated showed sensitivity to oxacillin/cefoxitin and the diagnostic images show a visual description of the resistance different groups formed, that may be related to sensitivity and resistance to oxacillin/cefoxitin, characterizing the MRSA <i>S. aureus</i>. Conclusions: Samples that showed phenotypic resistance to oxacillin/cefoxitin were clearly separated from samples that did not show this resistance. A PLS-DA model predicted the presence of resistance to oxacillin/cefoxitin in <i>S. aureus</i> samples and it was possible to observe the pixels classified as MRSA. The HSI was able to successfully discriminate samples in replicas that were sensitive and resistant, based on the calibration model it received.https://www.mdpi.com/2076-2607/11/3/615bacteriadiagnostic imagingchildrenantibiogramcontamination |
| spellingShingle | Aline Cristine Magalhães Costa Messias Aline Rodrigues Gama Letícia Suriano de Almeida Prado Paulo Alex Neves da Silva Arlindo Rodrigues Galvão Filho Clarimar José Coelho Melissa Ameloti Gomes Avelino José Daniel Gonçalves Vieira Lilian Carla Carneiro Detection of Oxacillin/Cefoxitin Resistance in <i>Staphylococcus aureus</i> Present in Recurrent Tonsillitis Microorganisms bacteria diagnostic imaging children antibiogram contamination |
| title | Detection of Oxacillin/Cefoxitin Resistance in <i>Staphylococcus aureus</i> Present in Recurrent Tonsillitis |
| title_full | Detection of Oxacillin/Cefoxitin Resistance in <i>Staphylococcus aureus</i> Present in Recurrent Tonsillitis |
| title_fullStr | Detection of Oxacillin/Cefoxitin Resistance in <i>Staphylococcus aureus</i> Present in Recurrent Tonsillitis |
| title_full_unstemmed | Detection of Oxacillin/Cefoxitin Resistance in <i>Staphylococcus aureus</i> Present in Recurrent Tonsillitis |
| title_short | Detection of Oxacillin/Cefoxitin Resistance in <i>Staphylococcus aureus</i> Present in Recurrent Tonsillitis |
| title_sort | detection of oxacillin cefoxitin resistance in i staphylococcus aureus i present in recurrent tonsillitis |
| topic | bacteria diagnostic imaging children antibiogram contamination |
| url | https://www.mdpi.com/2076-2607/11/3/615 |
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