Different Effects of Quercetin Glycosides and Quercetin on Kidney Mitochondrial Function—Uncoupling, Cytochrome C Reducing and Antioxidant Activity
Flavonols are found in plants as aglycones and as glycosides. Antioxidant activity of flavonols may occur via several mechanisms within the cell, and mitochondria as a target may play an important role. There is a lack of information about the influence of the sugar moiety on biological activity of...
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/19/6377 |
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| author | Kristina Zymone Raimondas Benetis Darius Trumbeckas Ingrida Baseviciene Sonata Trumbeckaite |
| author_facet | Kristina Zymone Raimondas Benetis Darius Trumbeckas Ingrida Baseviciene Sonata Trumbeckaite |
| author_sort | Kristina Zymone |
| collection | DOAJ |
| description | Flavonols are found in plants as aglycones and as glycosides. Antioxidant activity of flavonols may occur via several mechanisms within the cell, and mitochondria as a target may play an important role. There is a lack of information about the influence of the sugar moiety on biological activity of flavonoid glycosides. The aims of study were to investigate the effects of quercetin and its glycosides on mitochondrial respiration rates at various metabolic states, and to evaluate their antioxidant potential using chemical and biological approaches. Mitochondrial function was measured using an oxygraphic method, cytochrome c reduction spectrophotometrically, H<sub>2</sub>O<sub>2</sub> generation in mitochondria fluorimetrically, and antioxidant activity of flavonoids using an HPLC-post column system. Our data revealed that quercetin and its glycosides isoquercitrin, rutin, and hyperoside uncouple kidney mitochondrial respiration (increasing the State 2 respiration rate) and significantly reduce cytochrome c. Moreover, quercetin, and its glycosides decrease the production of mitochondrial H<sub>2</sub>O<sub>2</sub> and possess radical scavenging and ferric reducing capacities. The highest activity was characteristic for quercetin, showing that the sugar moiety significantly diminishes its activity. In conclusion, our results show the efficient radical scavenging, ferric and cytochrome c reducing capacities, and uncoupling properties of quercetin and its glycosides, as well as the importance of the sugar residue and its structure in the regulation of kidney mitochondrial function. |
| first_indexed | 2024-03-09T21:26:03Z |
| format | Article |
| id | doaj.art-cd21905a3ffa4780b69e3dce40a8e756 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:26:03Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cd21905a3ffa4780b69e3dce40a8e7562023-11-23T21:10:27ZengMDPI AGMolecules1420-30492022-09-012719637710.3390/molecules27196377Different Effects of Quercetin Glycosides and Quercetin on Kidney Mitochondrial Function—Uncoupling, Cytochrome C Reducing and Antioxidant ActivityKristina Zymone0Raimondas Benetis1Darius Trumbeckas2Ingrida Baseviciene3Sonata Trumbeckaite4Laboratory of Biopharmaceutical Research, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, LithuaniaDepartment of Drug Chemistry, Faculty of Pharmacy, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, LithuaniaDepartment of Urology, Medical Academy, Lithuanian University of Health Sciences, Eivenių G. 2, LT-50009 Kaunas, LithuaniaDepartment of Obstetrics and Gynaecology, Lithuanian University of Health Sciences, Eiveniu Str. 2, LT-50009 Kaunas, LithuaniaNeuroscience Institute, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, LithuaniaFlavonols are found in plants as aglycones and as glycosides. Antioxidant activity of flavonols may occur via several mechanisms within the cell, and mitochondria as a target may play an important role. There is a lack of information about the influence of the sugar moiety on biological activity of flavonoid glycosides. The aims of study were to investigate the effects of quercetin and its glycosides on mitochondrial respiration rates at various metabolic states, and to evaluate their antioxidant potential using chemical and biological approaches. Mitochondrial function was measured using an oxygraphic method, cytochrome c reduction spectrophotometrically, H<sub>2</sub>O<sub>2</sub> generation in mitochondria fluorimetrically, and antioxidant activity of flavonoids using an HPLC-post column system. Our data revealed that quercetin and its glycosides isoquercitrin, rutin, and hyperoside uncouple kidney mitochondrial respiration (increasing the State 2 respiration rate) and significantly reduce cytochrome c. Moreover, quercetin, and its glycosides decrease the production of mitochondrial H<sub>2</sub>O<sub>2</sub> and possess radical scavenging and ferric reducing capacities. The highest activity was characteristic for quercetin, showing that the sugar moiety significantly diminishes its activity. In conclusion, our results show the efficient radical scavenging, ferric and cytochrome c reducing capacities, and uncoupling properties of quercetin and its glycosides, as well as the importance of the sugar residue and its structure in the regulation of kidney mitochondrial function.https://www.mdpi.com/1420-3049/27/19/6377quercetinrutinhyperosideisoquercitrinkidney mitochondriarespiration rates |
| spellingShingle | Kristina Zymone Raimondas Benetis Darius Trumbeckas Ingrida Baseviciene Sonata Trumbeckaite Different Effects of Quercetin Glycosides and Quercetin on Kidney Mitochondrial Function—Uncoupling, Cytochrome C Reducing and Antioxidant Activity Molecules quercetin rutin hyperoside isoquercitrin kidney mitochondria respiration rates |
| title | Different Effects of Quercetin Glycosides and Quercetin on Kidney Mitochondrial Function—Uncoupling, Cytochrome C Reducing and Antioxidant Activity |
| title_full | Different Effects of Quercetin Glycosides and Quercetin on Kidney Mitochondrial Function—Uncoupling, Cytochrome C Reducing and Antioxidant Activity |
| title_fullStr | Different Effects of Quercetin Glycosides and Quercetin on Kidney Mitochondrial Function—Uncoupling, Cytochrome C Reducing and Antioxidant Activity |
| title_full_unstemmed | Different Effects of Quercetin Glycosides and Quercetin on Kidney Mitochondrial Function—Uncoupling, Cytochrome C Reducing and Antioxidant Activity |
| title_short | Different Effects of Quercetin Glycosides and Quercetin on Kidney Mitochondrial Function—Uncoupling, Cytochrome C Reducing and Antioxidant Activity |
| title_sort | different effects of quercetin glycosides and quercetin on kidney mitochondrial function uncoupling cytochrome c reducing and antioxidant activity |
| topic | quercetin rutin hyperoside isoquercitrin kidney mitochondria respiration rates |
| url | https://www.mdpi.com/1420-3049/27/19/6377 |
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