Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
This study was conducted to assess whether Lactobacillus-containing probiotics could protect intestinal mucosa in rats during traumatic hemorrhagic shock and to determine its underlying mechanisms. Healthy male Sprague–Dawley rats (300 ± 20 g) were randomly divided into four groups. During the study...
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| Format: | Article |
| Language: | English |
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De Gruyter
2021-10-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2021-0112 |
| _version_ | 1797998064756064256 |
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| author | Wang Lei Liu Shu-li Xu Zhi-peng Song Qi Li Lei Qiu Zhao-lei Wang Zhen-jie |
| author_facet | Wang Lei Liu Shu-li Xu Zhi-peng Song Qi Li Lei Qiu Zhao-lei Wang Zhen-jie |
| author_sort | Wang Lei |
| collection | DOAJ |
| description | This study was conducted to assess whether Lactobacillus-containing probiotics could protect intestinal mucosa in rats during traumatic hemorrhagic shock and to determine its underlying mechanisms. Healthy male Sprague–Dawley rats (300 ± 20 g) were randomly divided into four groups. During the study, reverse transcription polymerase chain reaction, western blotting, and hematoxylin and eosin methods were used. There was a significant increase in the expression of toll-like receptor 4 (TLR4) in the rats that experienced traumatic hemorrhagic shock, along with increased mRNA of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6. Pretreatment with Lactobacillus-containing probiotics reduced TLR4 expression, decreased phosphorylation (Ser536) and acetylation (Lys310) of p65, and decreased TNF-α and IL-6 mRNA. The probiotics combined acetate Ringer’s group showed a less severe pathological manifestation compared to the other experimental groups. Lactobacillus-containing probiotics inhibited nuclear factor-kappa B signaling via the downregulation of TLR4, resulting in inflammatory homeostasis, which might be the mechanism whereby Lactobacillus protects the intestinal mucosa from damage caused by the traumatic hemorrhagic shock. |
| first_indexed | 2024-04-11T10:42:33Z |
| format | Article |
| id | doaj.art-cd21fca2d5fc4e1d8a39ca4fb416f7c6 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-04-11T10:42:33Z |
| publishDate | 2021-10-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-cd21fca2d5fc4e1d8a39ca4fb416f7c62022-12-22T04:29:08ZengDe GruyterOpen Life Sciences2391-54122021-10-011611122112910.1515/biol-2021-0112Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shockWang Lei0Liu Shu-li1Xu Zhi-peng2Song Qi3Li Lei4Qiu Zhao-lei5Wang Zhen-jie6Department of Emergency, Anhui Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, ChinaDepartment of Intensive Care Unit, The Second Affiliated Hospital of Shandong First Medical University, Tai’an, Shandong, 271000, ChinaDepartment of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, 233004, ChinaDepartment of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, 233004, ChinaDepartment of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, 233004, ChinaDepartment of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, 233004, ChinaDepartment of Emergency Surgery, The First Affiliated Hospital of Bengbu Medical College, No. 287, Changhuai Road, Bengbu, Anhui, 233004, ChinaThis study was conducted to assess whether Lactobacillus-containing probiotics could protect intestinal mucosa in rats during traumatic hemorrhagic shock and to determine its underlying mechanisms. Healthy male Sprague–Dawley rats (300 ± 20 g) were randomly divided into four groups. During the study, reverse transcription polymerase chain reaction, western blotting, and hematoxylin and eosin methods were used. There was a significant increase in the expression of toll-like receptor 4 (TLR4) in the rats that experienced traumatic hemorrhagic shock, along with increased mRNA of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6. Pretreatment with Lactobacillus-containing probiotics reduced TLR4 expression, decreased phosphorylation (Ser536) and acetylation (Lys310) of p65, and decreased TNF-α and IL-6 mRNA. The probiotics combined acetate Ringer’s group showed a less severe pathological manifestation compared to the other experimental groups. Lactobacillus-containing probiotics inhibited nuclear factor-kappa B signaling via the downregulation of TLR4, resulting in inflammatory homeostasis, which might be the mechanism whereby Lactobacillus protects the intestinal mucosa from damage caused by the traumatic hemorrhagic shock.https://doi.org/10.1515/biol-2021-0112compound lactobacillus traumamucosal integritynf-κb signaling pathway |
| spellingShingle | Wang Lei Liu Shu-li Xu Zhi-peng Song Qi Li Lei Qiu Zhao-lei Wang Zhen-jie Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock Open Life Sciences compound lactobacillus trauma mucosal integrity nf-κb signaling pathway |
| title | Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock |
| title_full | Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock |
| title_fullStr | Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock |
| title_full_unstemmed | Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock |
| title_short | Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock |
| title_sort | protective effect of lactobacillus containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock |
| topic | compound lactobacillus trauma mucosal integrity nf-κb signaling pathway |
| url | https://doi.org/10.1515/biol-2021-0112 |
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