A two-dimensional pooling strategy for rare variant detection on next-generation sequencing platforms.

A two-dimensional pooling strategy for rare variant detection on next-generation sequencing platforms.

We describe a method for pooling and sequencing DNA from a large number of individual samples while preserving information regarding sample identity. DNA from 576 individuals was arranged into four 12 row by 12 column matrices and then pooled by row and by column resulting in 96 total pools with 12...

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Bibliographic Details
Main Authors:	Philip C Zuzarte, Robert E Denroche, Gordon Fehringer, Hagit Katzov-Eckert, Rayjean J Hung, John D McPherson
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2014-01-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC3984111?pdf=render

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