Synthetic and Nanotechnological Approaches for a Diagnostic Use of Manganese
The development of multimodal imaging techniques such as positron emission tomography (PET) and magnetic resonance imaging (MRI) allows the contemporary obtaining of metabolic and morphological information. To fully exploit the complementarity of the two imaging modalities, the design of probes disp...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/10/3124 |
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| author | Maddalena Sguizzato Petra Martini Lorenza Marvelli Walter Pula Markus Drechsler Martina Capozza Enzo Terreno Lucia Del Bianco Federico Spizzo Rita Cortesi Alessandra Boschi |
| author_facet | Maddalena Sguizzato Petra Martini Lorenza Marvelli Walter Pula Markus Drechsler Martina Capozza Enzo Terreno Lucia Del Bianco Federico Spizzo Rita Cortesi Alessandra Boschi |
| author_sort | Maddalena Sguizzato |
| collection | DOAJ |
| description | The development of multimodal imaging techniques such as positron emission tomography (PET) and magnetic resonance imaging (MRI) allows the contemporary obtaining of metabolic and morphological information. To fully exploit the complementarity of the two imaging modalities, the design of probes displaying radioactive and magnetic properties at the same time could be very beneficial. In this regard, transition metals offer appealing options, with manganese representing an ideal candidate. As nanosized imaging probes have demonstrated great value for designing advanced diagnostic/theranostic procedures, this work focuses on the potential of liposomal formulations loaded with a new synthesized paramagnetic Mn(II) chelates. Negatively charged liposomes were produced by thin-layer hydration method and extrusion. The obtained formulations were characterized in terms of size, surface charge, efficiency of encapsulation, stability over time, relaxivity, effective magnetic moment, and in vitro antiproliferative effect on human cells by means of the MTT assay. The negatively charged paramagnetic liposomes were monodisperse, with an average hydrodynamic diameter not exceeding 200 nm, and they displayed good stability and no cytotoxicity. As determined by optical emission spectroscopy, manganese complexes are loaded almost completely on liposomes maintaining their paramagnetic properties. |
| first_indexed | 2024-03-10T03:20:32Z |
| format | Article |
| id | doaj.art-cd24ad0b06dd48208672d4846e0e18bc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:20:32Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cd24ad0b06dd48208672d4846e0e18bc2023-11-23T12:21:26ZengMDPI AGMolecules1420-30492022-05-012710312410.3390/molecules27103124Synthetic and Nanotechnological Approaches for a Diagnostic Use of ManganeseMaddalena Sguizzato0Petra Martini1Lorenza Marvelli2Walter Pula3Markus Drechsler4Martina Capozza5Enzo Terreno6Lucia Del Bianco7Federico Spizzo8Rita Cortesi9Alessandra Boschi10Department of Chemical, Pharmaceutical and Agricultural Sciences (DoCPAS), University of Ferrara, 44121 Ferrara, ItalyDepartment of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, ItalyDepartment of Chemical, Pharmaceutical and Agricultural Sciences (DoCPAS), University of Ferrara, 44121 Ferrara, ItalyDepartment of Chemical, Pharmaceutical and Agricultural Sciences (DoCPAS), University of Ferrara, 44121 Ferrara, ItalyKey Lab “Electron and Optical Microscopy”, Bavarian Polymer Institute (BPI), University of Bayreuth, 95440 Bayreuth, GermanyMolecular & Preclinical Imaging Center, Department of Molecular Biotechnologies and Health Sciences, University of Torino, 10126 Torino, ItalyMolecular & Preclinical Imaging Center, Department of Molecular Biotechnologies and Health Sciences, University of Torino, 10126 Torino, ItalyDepartment of Physics and Earth Science, University of Ferrara, 44122 Ferrara, ItalyDepartment of Physics and Earth Science, University of Ferrara, 44122 Ferrara, ItalyDepartment of Chemical, Pharmaceutical and Agricultural Sciences (DoCPAS), University of Ferrara, 44121 Ferrara, ItalyDepartment of Chemical, Pharmaceutical and Agricultural Sciences (DoCPAS), University of Ferrara, 44121 Ferrara, ItalyThe development of multimodal imaging techniques such as positron emission tomography (PET) and magnetic resonance imaging (MRI) allows the contemporary obtaining of metabolic and morphological information. To fully exploit the complementarity of the two imaging modalities, the design of probes displaying radioactive and magnetic properties at the same time could be very beneficial. In this regard, transition metals offer appealing options, with manganese representing an ideal candidate. As nanosized imaging probes have demonstrated great value for designing advanced diagnostic/theranostic procedures, this work focuses on the potential of liposomal formulations loaded with a new synthesized paramagnetic Mn(II) chelates. Negatively charged liposomes were produced by thin-layer hydration method and extrusion. The obtained formulations were characterized in terms of size, surface charge, efficiency of encapsulation, stability over time, relaxivity, effective magnetic moment, and in vitro antiproliferative effect on human cells by means of the MTT assay. The negatively charged paramagnetic liposomes were monodisperse, with an average hydrodynamic diameter not exceeding 200 nm, and they displayed good stability and no cytotoxicity. As determined by optical emission spectroscopy, manganese complexes are loaded almost completely on liposomes maintaining their paramagnetic properties.https://www.mdpi.com/1420-3049/27/10/3124lipid-based nanosystemsliposomesmanganesePET/MRImagnetic susceptibility |
| spellingShingle | Maddalena Sguizzato Petra Martini Lorenza Marvelli Walter Pula Markus Drechsler Martina Capozza Enzo Terreno Lucia Del Bianco Federico Spizzo Rita Cortesi Alessandra Boschi Synthetic and Nanotechnological Approaches for a Diagnostic Use of Manganese Molecules lipid-based nanosystems liposomes manganese PET/MRI magnetic susceptibility |
| title | Synthetic and Nanotechnological Approaches for a Diagnostic Use of Manganese |
| title_full | Synthetic and Nanotechnological Approaches for a Diagnostic Use of Manganese |
| title_fullStr | Synthetic and Nanotechnological Approaches for a Diagnostic Use of Manganese |
| title_full_unstemmed | Synthetic and Nanotechnological Approaches for a Diagnostic Use of Manganese |
| title_short | Synthetic and Nanotechnological Approaches for a Diagnostic Use of Manganese |
| title_sort | synthetic and nanotechnological approaches for a diagnostic use of manganese |
| topic | lipid-based nanosystems liposomes manganese PET/MRI magnetic susceptibility |
| url | https://www.mdpi.com/1420-3049/27/10/3124 |
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