Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925
Kluyveromyces marxianus TISTR 5925, isolated from rotten fruit in Thailand, can ferment at pH 3 at temperatures between 42 and 45 ℃. Bioethanol production from cassava pulp using the simultaneous saccharification and fermentation (SSF) process was evaluated and compared with the separated hydrolysis...
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| Format: | Article |
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AIMS Press
2013-10-01
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| Series: | AIMS Energy |
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| Online Access: | http://www.aimspress.com/article/10.3934/energy.2013.1.3/fulltext.html |
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| author | Waraporn Apiwatanapiwat Prapassorn Rugthaworn Pilanee Vaithanomsat Warunee Thanapase Akihiko Kosugi Takamitsu Arai Yutaka Mori Yoshinori Murata |
| author_facet | Waraporn Apiwatanapiwat Prapassorn Rugthaworn Pilanee Vaithanomsat Warunee Thanapase Akihiko Kosugi Takamitsu Arai Yutaka Mori Yoshinori Murata |
| author_sort | Waraporn Apiwatanapiwat |
| collection | DOAJ |
| description | Kluyveromyces marxianus TISTR 5925, isolated from rotten fruit in Thailand, can ferment at pH 3 at temperatures between 42 and 45 ℃. Bioethanol production from cassava pulp using the simultaneous saccharification and fermentation (SSF) process was evaluated and compared with the separated hydrolysis and fermentation (SHF) process using K. marxianus TISTR 5925. The ethanol concentrations obtained from the SSF process were higher than those from the SHF process. The optimum conditions for ethanol production were investigated by response surface methodology (RSM) based on a five level central composite design involving the following variables: enzyme dilution (times), temperature (℃) and fermentation time (h). Cassava pulp was pretreated by boiling for 10 min, treated with a mixture of enzymes (cellulase, pectinase, α-amylase and glucoamylase), then fermented by K. marxianus TISTR 5925. Data obtained from the RSM were subjected to analysis of variance and fit to a second order polynomial equation. At optimum enzyme dilution (0.1 times), temperature (41 ℃) and fermentation time (27 h), the maximum obtained concentration of ethanol was 5.0% (w/v), which is very close to the predicted ethanol concentration of 5.3% (w/v). |
| first_indexed | 2024-12-11T18:56:20Z |
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| id | doaj.art-cd279ea92b614ca28854b4ed12014e23 |
| institution | Directory Open Access Journal |
| issn | 2333-8326 2333-8334 |
| language | English |
| last_indexed | 2024-12-11T18:56:20Z |
| publishDate | 2013-10-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Energy |
| spelling | doaj.art-cd279ea92b614ca28854b4ed12014e232022-12-22T00:54:09ZengAIMS PressAIMS Energy2333-83262333-83342013-10-011131610.3934/energy.2013.1.3Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925Waraporn Apiwatanapiwat0Prapassorn Rugthaworn1Pilanee Vaithanomsat2Warunee Thanapase3Akihiko Kosugi4Takamitsu Arai5Yutaka Mori6Yoshinori Murata7Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, JapanJapan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, JapanKasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50 Chatuchak, Bangkok 10900, ThailandKasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50 Chatuchak, Bangkok 10900, ThailandJapan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, JapanJapan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, JapanJapan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, JapanJapan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, JapanKluyveromyces marxianus TISTR 5925, isolated from rotten fruit in Thailand, can ferment at pH 3 at temperatures between 42 and 45 ℃. Bioethanol production from cassava pulp using the simultaneous saccharification and fermentation (SSF) process was evaluated and compared with the separated hydrolysis and fermentation (SHF) process using K. marxianus TISTR 5925. The ethanol concentrations obtained from the SSF process were higher than those from the SHF process. The optimum conditions for ethanol production were investigated by response surface methodology (RSM) based on a five level central composite design involving the following variables: enzyme dilution (times), temperature (℃) and fermentation time (h). Cassava pulp was pretreated by boiling for 10 min, treated with a mixture of enzymes (cellulase, pectinase, α-amylase and glucoamylase), then fermented by K. marxianus TISTR 5925. Data obtained from the RSM were subjected to analysis of variance and fit to a second order polynomial equation. At optimum enzyme dilution (0.1 times), temperature (41 ℃) and fermentation time (27 h), the maximum obtained concentration of ethanol was 5.0% (w/v), which is very close to the predicted ethanol concentration of 5.3% (w/v).http://www.aimspress.com/article/10.3934/energy.2013.1.3/fulltext.htmlEthanolThermotolerantResponse surface methodologyCassava pulp |
| spellingShingle | Waraporn Apiwatanapiwat Prapassorn Rugthaworn Pilanee Vaithanomsat Warunee Thanapase Akihiko Kosugi Takamitsu Arai Yutaka Mori Yoshinori Murata Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925 AIMS Energy Ethanol Thermotolerant Response surface methodology Cassava pulp |
| title | Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925 |
| title_full | Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925 |
| title_fullStr | Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925 |
| title_full_unstemmed | Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925 |
| title_short | Ethanol production at high temperature from cassava pulp by a newly isolated Kluyveromyces marxianus strain, TISTR 5925 |
| title_sort | ethanol production at high temperature from cassava pulp by a newly isolated kluyveromyces marxianus strain tistr 5925 |
| topic | Ethanol Thermotolerant Response surface methodology Cassava pulp |
| url | http://www.aimspress.com/article/10.3934/energy.2013.1.3/fulltext.html |
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