Determination of biofilm formation, antibacterial resistance and genotypes of bacillus cereus isolates from raw milk
Bacillus cereus is a foodborne pathogen that has a widespread presence in the environment and frequently found in foods especially in dairy products. Raw milk contaminated with B. cereus could be the cause of its widespreadness in the environment. In this study, it was aimed to determine the genotyp...
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Format: | Article |
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Kafkas University, Faculty of Veterinary Medicine
2023-05-01
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Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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Online Access: | https://vetdergikafkas.org/pdf.php?id=2999 |
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author | Sadık SAVAŞAN Çağatay NUHAY Volkan Enes ERGÜDEN Serap SAVAŞAN |
author_facet | Sadık SAVAŞAN Çağatay NUHAY Volkan Enes ERGÜDEN Serap SAVAŞAN |
author_sort | Sadık SAVAŞAN |
collection | DOAJ |
description | Bacillus cereus is a foodborne pathogen that has a widespread presence in the environment and frequently found in foods
especially in dairy products. Raw milk contaminated with B. cereus could be the cause of its widespreadness in the environment. In this
study, it was aimed to determine the genotypes, biofilm formation, antimicrobial susceptibilities, and antibiotypes of B. cereus isolates from
raw milk. For this aim, B. cereus isolated and identified from 10 of 250 raw milk samples were investigated. Biofilm forming abilities were
determined in vitro by Congo Red Agar Method. Kirby Bauer Disc Diffusion Method was used for determining the antibiotic susceptibilities
of the isolates. According to the antibiotic susceptibility results, quantitative antibiotyping was implemented. Genotyping of the isolates
were performed by RAPD-PCR. Biofilm formation was determined in 40% of the isolates. The resistances against amoxicillin-clavulanic
acid, gentamicin, erythromycin, vancomycin, chloramphenicol, tetracycline, trimethoprim-sulfamethoxazole were determined in 100%,
0%, 30%, 0%, 0%, 0%, and 50% of the isolates, respectively. In the quantitative antibiotyping, the isolates showed similarity between 0.75 to
1.00. The phylogenetic similarities were calculated between 29% to 82%. In conclusion, raw milks might threat the public health because of
having potential of containing the antibiotic resistant B. cereus. |
first_indexed | 2024-03-12T10:24:05Z |
format | Article |
id | doaj.art-cd2c4c13b8e84df69176251a8ed012e7 |
institution | Directory Open Access Journal |
issn | 1309-2251 |
language | English |
last_indexed | 2024-03-12T10:24:05Z |
publishDate | 2023-05-01 |
publisher | Kafkas University, Faculty of Veterinary Medicine |
record_format | Article |
series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
spelling | doaj.art-cd2c4c13b8e84df69176251a8ed012e72023-09-02T09:54:26ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-05-0129326527110.9775/kvfd.2023.291622999Determination of biofilm formation, antibacterial resistance and genotypes of bacillus cereus isolates from raw milkSadık SAVAŞAN0Çağatay NUHAY1Volkan Enes ERGÜDEN2Serap SAVAŞAN3Aydın Adnan Menderes University, Graduate School of Health Sciences, Food Hygiene and Technology Department, TR- 09100 Aydın - TÜRKİYEBornova Veterinary Control Institute, Bacteriology Laboratory, TR-35040 İzmir - TÜRKİYEOndokuz Mayıs University, Faculty of Veterinary Medicine, Veterinary Microbiology Department, TR-55200 Samsun - TÜRKİYEAydın Adnan Menderes University, Faculty of Veterinary Medicine, Veterinary Microbiology Department, TR-09100 Aydın - TÜRKİYEBacillus cereus is a foodborne pathogen that has a widespread presence in the environment and frequently found in foods especially in dairy products. Raw milk contaminated with B. cereus could be the cause of its widespreadness in the environment. In this study, it was aimed to determine the genotypes, biofilm formation, antimicrobial susceptibilities, and antibiotypes of B. cereus isolates from raw milk. For this aim, B. cereus isolated and identified from 10 of 250 raw milk samples were investigated. Biofilm forming abilities were determined in vitro by Congo Red Agar Method. Kirby Bauer Disc Diffusion Method was used for determining the antibiotic susceptibilities of the isolates. According to the antibiotic susceptibility results, quantitative antibiotyping was implemented. Genotyping of the isolates were performed by RAPD-PCR. Biofilm formation was determined in 40% of the isolates. The resistances against amoxicillin-clavulanic acid, gentamicin, erythromycin, vancomycin, chloramphenicol, tetracycline, trimethoprim-sulfamethoxazole were determined in 100%, 0%, 30%, 0%, 0%, 0%, and 50% of the isolates, respectively. In the quantitative antibiotyping, the isolates showed similarity between 0.75 to 1.00. The phylogenetic similarities were calculated between 29% to 82%. In conclusion, raw milks might threat the public health because of having potential of containing the antibiotic resistant B. cereus.https://vetdergikafkas.org/pdf.php?id=2999antibacterial resistanceb. cereusbiofilmgenotypingraw milk |
spellingShingle | Sadık SAVAŞAN Çağatay NUHAY Volkan Enes ERGÜDEN Serap SAVAŞAN Determination of biofilm formation, antibacterial resistance and genotypes of bacillus cereus isolates from raw milk Kafkas Universitesi Veteriner Fakültesi Dergisi antibacterial resistance b. cereus biofilm genotyping raw milk |
title | Determination of biofilm formation, antibacterial resistance and genotypes of bacillus cereus isolates from raw milk |
title_full | Determination of biofilm formation, antibacterial resistance and genotypes of bacillus cereus isolates from raw milk |
title_fullStr | Determination of biofilm formation, antibacterial resistance and genotypes of bacillus cereus isolates from raw milk |
title_full_unstemmed | Determination of biofilm formation, antibacterial resistance and genotypes of bacillus cereus isolates from raw milk |
title_short | Determination of biofilm formation, antibacterial resistance and genotypes of bacillus cereus isolates from raw milk |
title_sort | determination of biofilm formation antibacterial resistance and genotypes of bacillus cereus isolates from raw milk |
topic | antibacterial resistance b. cereus biofilm genotyping raw milk |
url | https://vetdergikafkas.org/pdf.php?id=2999 |
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