Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1
Pronounced inflammation after intracerebral hemorrhage (ICH) contributes to secondary brain injury, which seriously affects the prognosis of ICH patients. Fractalkine (FKN)/CX3CR1 pathway may play an important role in regulating inflammatory reactions after ICH. As resident immunocyte in brain, micr...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2020-09-01
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| Series: | Brain Hemorrhages |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589238X20300292 |
| _version_ | 1819289294142963712 |
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| author | Hai-Han Yu Gai-Gai Li Ying-Xin Tang Shuang Bai Chao Pan Zhou-Ping Tang |
| author_facet | Hai-Han Yu Gai-Gai Li Ying-Xin Tang Shuang Bai Chao Pan Zhou-Ping Tang |
| author_sort | Hai-Han Yu |
| collection | DOAJ |
| description | Pronounced inflammation after intracerebral hemorrhage (ICH) contributes to secondary brain injury, which seriously affects the prognosis of ICH patients. Fractalkine (FKN)/CX3CR1 pathway may play an important role in regulating inflammatory reactions after ICH. As resident immunocyte in brain, microglia is essential for inflammatory reactions in central nervous system. This study intends to explore the effect of FKN/CX3CR1 pathway on microglia after experimental ICH and its possible mechanisms. In mouse ICH models, FKN and TGF-β1 levels gradually increased and showed a positive correlation, both of which peaked at the third day after ICH model. Compared with ICH only, the ratio of s-FKN/m-FKN, microglia activation, and neuronal apoptosis were all decreased after injecting ADAM10 inhibitor (G1254023X) into the lateral ventricle. The expression of inflammatory factors was increased after stimulation with hemin in BV2 cells. The addition of m-FKN reduced the inflammatory response to a certain extent, and the inhibitor of TGF-β1 could counteract the effect of m-FKN.In conclusion, in mouse ICH model, the expression of FKN is increased, and m-FKN may inhibit the activation of microglia and reduce neuron apoptosis by promoting the expression of TGF-β1. |
| first_indexed | 2024-12-24T03:04:33Z |
| format | Article |
| id | doaj.art-cd35ebf0c27942ef93fc4827afc21c34 |
| institution | Directory Open Access Journal |
| issn | 2589-238X |
| language | English |
| last_indexed | 2024-12-24T03:04:33Z |
| publishDate | 2020-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Brain Hemorrhages |
| spelling | doaj.art-cd35ebf0c27942ef93fc4827afc21c342022-12-21T17:18:03ZengKeAi Communications Co., Ltd.Brain Hemorrhages2589-238X2020-09-0113146151Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1Hai-Han Yu0Gai-Gai Li1Ying-Xin Tang2Shuang Bai3Chao Pan4Zhou-Ping Tang5Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaCorresponding authors at: Department of Neurology, Tongji hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, Hubei 430030, China.; Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaCorresponding authors at: Department of Neurology, Tongji hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, Hubei 430030, China.; Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaPronounced inflammation after intracerebral hemorrhage (ICH) contributes to secondary brain injury, which seriously affects the prognosis of ICH patients. Fractalkine (FKN)/CX3CR1 pathway may play an important role in regulating inflammatory reactions after ICH. As resident immunocyte in brain, microglia is essential for inflammatory reactions in central nervous system. This study intends to explore the effect of FKN/CX3CR1 pathway on microglia after experimental ICH and its possible mechanisms. In mouse ICH models, FKN and TGF-β1 levels gradually increased and showed a positive correlation, both of which peaked at the third day after ICH model. Compared with ICH only, the ratio of s-FKN/m-FKN, microglia activation, and neuronal apoptosis were all decreased after injecting ADAM10 inhibitor (G1254023X) into the lateral ventricle. The expression of inflammatory factors was increased after stimulation with hemin in BV2 cells. The addition of m-FKN reduced the inflammatory response to a certain extent, and the inhibitor of TGF-β1 could counteract the effect of m-FKN.In conclusion, in mouse ICH model, the expression of FKN is increased, and m-FKN may inhibit the activation of microglia and reduce neuron apoptosis by promoting the expression of TGF-β1.http://www.sciencedirect.com/science/article/pii/S2589238X20300292Intracerebral hemorrhageMicrogliaFractalkineTGF-β1BV2 |
| spellingShingle | Hai-Han Yu Gai-Gai Li Ying-Xin Tang Shuang Bai Chao Pan Zhou-Ping Tang Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1 Brain Hemorrhages Intracerebral hemorrhage Microglia Fractalkine TGF-β1 BV2 |
| title | Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1 |
| title_full | Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1 |
| title_fullStr | Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1 |
| title_full_unstemmed | Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1 |
| title_short | Fractalkine/CX3CR1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of TGF-β1 |
| title_sort | fractalkine cx3cr1 pathway is neuroprotective in intracerebral hemorrhage through facilitating the expression of tgf β1 |
| topic | Intracerebral hemorrhage Microglia Fractalkine TGF-β1 BV2 |
| url | http://www.sciencedirect.com/science/article/pii/S2589238X20300292 |
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