A nuclear circularity-based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological images
Context: Distinction of spindle cell melanoma (SM) and desmoplastic melanoma (DM) is clinically important due to differences in metastatic rate and prognosis; however, histological distinction is not always straightforward. During a routine review of cases, we noted differences in nuclear circularit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Journal of Pathology Informatics |
| Subjects: | |
| Online Access: | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2014;volume=5;issue=1;spage=40;epage=40;aulast=Schöchlin |
| _version_ | 1818554339313057792 |
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| author | Manuel Schöchlin Stephanie E Weissinger Arnd R Brandes Markus Herrmann Peter Möller Jochen K Lennerz |
| author_facet | Manuel Schöchlin Stephanie E Weissinger Arnd R Brandes Markus Herrmann Peter Möller Jochen K Lennerz |
| author_sort | Manuel Schöchlin |
| collection | DOAJ |
| description | Context: Distinction of spindle cell melanoma (SM) and desmoplastic melanoma (DM) is clinically important due to differences in metastatic rate and prognosis; however, histological distinction is not always straightforward. During a routine review of cases, we noted differences in nuclear circularity between SM and DM. Aim: The primary aim in our study was to determine whether these differences in nuclear circularity, when assessed using a basic ImageJ-based threshold extraction, can serve as a diagnostic classifier to distinguish DM from SM. Settings and Design: Our retrospective analysis of an established patient cohort (SM n = 9, DM n = 9) was employed to determine discriminatory power. Subjects and Methods: Regions of interest (total n = 108; 6 images per case) were selected from scanned H and E-stained histological sections, and nuclear circularity was extracted and quantified by computational image analysis using open source tools (plugins for ImageJ). Statistical Analysis: Using analysis of variance, t-tests, and Fisher′s exact tests, we compared extracted quantitative shape measures; statistical significance was defined as P < 0.05. Results: Classifying circularity values into four shape categories (spindled, elongated, oval, round) demonstrated significant differences in the spindled and round categories. Paradoxically, DM contained more spindled nuclei than SM (P = 0.011) and SM contained more round nuclei than DM (P = 0.026). Performance assessment using a combined shape-classification of the round and spindled fractions showed 88.9% accuracy and a Youden index of 0.77. Conclusions: Spindle cell melanoma and DM differ significantly in their nuclear morphology with respect to fractions of round and spindled nuclei. Our study demonstrates that quantifying nuclear circularity can be used as an adjunct diagnostic tool for distinction of DM and SM. |
| first_indexed | 2024-12-12T09:38:05Z |
| format | Article |
| id | doaj.art-cd36058a00214cf1aba05c15259bece4 |
| institution | Directory Open Access Journal |
| issn | 2153-3539 |
| language | English |
| last_indexed | 2024-12-12T09:38:05Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pathology Informatics |
| spelling | doaj.art-cd36058a00214cf1aba05c15259bece42022-12-22T00:28:40ZengElsevierJournal of Pathology Informatics2153-35392014-01-0151404010.4103/2153-3539.143335A nuclear circularity-based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological imagesManuel SchöchlinStephanie E WeissingerArnd R BrandesMarkus HerrmannPeter MöllerJochen K LennerzContext: Distinction of spindle cell melanoma (SM) and desmoplastic melanoma (DM) is clinically important due to differences in metastatic rate and prognosis; however, histological distinction is not always straightforward. During a routine review of cases, we noted differences in nuclear circularity between SM and DM. Aim: The primary aim in our study was to determine whether these differences in nuclear circularity, when assessed using a basic ImageJ-based threshold extraction, can serve as a diagnostic classifier to distinguish DM from SM. Settings and Design: Our retrospective analysis of an established patient cohort (SM n = 9, DM n = 9) was employed to determine discriminatory power. Subjects and Methods: Regions of interest (total n = 108; 6 images per case) were selected from scanned H and E-stained histological sections, and nuclear circularity was extracted and quantified by computational image analysis using open source tools (plugins for ImageJ). Statistical Analysis: Using analysis of variance, t-tests, and Fisher′s exact tests, we compared extracted quantitative shape measures; statistical significance was defined as P < 0.05. Results: Classifying circularity values into four shape categories (spindled, elongated, oval, round) demonstrated significant differences in the spindled and round categories. Paradoxically, DM contained more spindled nuclei than SM (P = 0.011) and SM contained more round nuclei than DM (P = 0.026). Performance assessment using a combined shape-classification of the round and spindled fractions showed 88.9% accuracy and a Youden index of 0.77. Conclusions: Spindle cell melanoma and DM differ significantly in their nuclear morphology with respect to fractions of round and spindled nuclei. Our study demonstrates that quantifying nuclear circularity can be used as an adjunct diagnostic tool for distinction of DM and SM.http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2014;volume=5;issue=1;spage=40;epage=40;aulast=SchöchlinDigital pathology, morphometry, numerical histology |
| spellingShingle | Manuel Schöchlin Stephanie E Weissinger Arnd R Brandes Markus Herrmann Peter Möller Jochen K Lennerz A nuclear circularity-based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological images Journal of Pathology Informatics Digital pathology, morphometry, numerical histology |
| title | A nuclear circularity-based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological images |
| title_full | A nuclear circularity-based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological images |
| title_fullStr | A nuclear circularity-based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological images |
| title_full_unstemmed | A nuclear circularity-based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological images |
| title_short | A nuclear circularity-based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological images |
| title_sort | nuclear circularity based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological images |
| topic | Digital pathology, morphometry, numerical histology |
| url | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2014;volume=5;issue=1;spage=40;epage=40;aulast=Schöchlin |
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