Characterization of culturable bacterial flora in yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with "gaping jaws" syndrome
One of the main problems facing Atlantic halibut hatcheries is the high mortality in the early stages of larval development. Several factors could be involved, for example: water quality, diseases or abnormalities, such as deformities occurring in the yolk sac larvae prior to exogenous feeding. The...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Escuela de Ciencias del Mar, Facultad de Recursos Naturales, Pontificia Universidad Católica de Valparaíso
2014-03-01
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| Series: | Latin American Journal of Aquatic Research |
| Subjects: | |
| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2014000100007&lng=en&tlng=en |
| _version_ | 1818649333598257152 |
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| author | Rocío Urtubia Pablo Gallardo Paris Lavin Nick Brown Marcelo González |
| author_facet | Rocío Urtubia Pablo Gallardo Paris Lavin Nick Brown Marcelo González |
| author_sort | Rocío Urtubia |
| collection | DOAJ |
| description | One of the main problems facing Atlantic halibut hatcheries is the high mortality in the early stages of larval development. Several factors could be involved, for example: water quality, diseases or abnormalities, such as deformities occurring in the yolk sac larvae prior to exogenous feeding. The aim of this study was to identify differences in bacterial flora associated with yolk sac larvae with oral deformity. We also aimed to establish whether there is any relationship between bacterial strains and the "gaping jaws" syndrome. During our study, 74 bacterial isolates were obtained using three different nutrient media: Marine Agar, R2A and TCBS. Some of these bacteria were characterized using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and 16S rRNA sequencing. The immune response in larvae exhibiting the "gaping jaws" condition was measured by real time PCR. Our results showed significant differences in bacterial flora between normal and gaping larvae. The gaping yolk sac larvae were predominantly colonized by members of the families Vibrionaceae and Flavobacteriaceae. Bacteria belonging to the Bacillus and Pseudoalteromonas genera were also present but less frequent. It was not possible to associate a type or group of bacteria directly related to "gaping". Strikingly, larvae with gaping jaws had an increase in the expression of two immune related genes, like hepcidin and chemokine (MIP-1B). These results indicate activation of the immune response in larvae with "gaping jaws" syndrome and this response could be related to bacteria isolated from gaping condition. |
| first_indexed | 2024-12-17T01:32:39Z |
| format | Article |
| id | doaj.art-cd3870d447944d7f830222b27c9deb2d |
| institution | Directory Open Access Journal |
| issn | 0718-560X |
| language | English |
| last_indexed | 2024-12-17T01:32:39Z |
| publishDate | 2014-03-01 |
| publisher | Escuela de Ciencias del Mar, Facultad de Recursos Naturales, Pontificia Universidad Católica de Valparaíso |
| record_format | Article |
| series | Latin American Journal of Aquatic Research |
| spelling | doaj.art-cd3870d447944d7f830222b27c9deb2d2022-12-21T22:08:31ZengEscuela de Ciencias del Mar, Facultad de Recursos Naturales, Pontificia Universidad Católica de ValparaísoLatin American Journal of Aquatic Research0718-560X2014-03-0142197110S0718-560X2014000100007Characterization of culturable bacterial flora in yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with "gaping jaws" syndromeRocío Urtubia0Pablo Gallardo1Paris Lavin2Nick Brown3Marcelo González4Instituto Antártico ChilenoUniversidad de MagallanesInstituto Antártico ChilenoUniversity of MaineInstituto Antártico ChilenoOne of the main problems facing Atlantic halibut hatcheries is the high mortality in the early stages of larval development. Several factors could be involved, for example: water quality, diseases or abnormalities, such as deformities occurring in the yolk sac larvae prior to exogenous feeding. The aim of this study was to identify differences in bacterial flora associated with yolk sac larvae with oral deformity. We also aimed to establish whether there is any relationship between bacterial strains and the "gaping jaws" syndrome. During our study, 74 bacterial isolates were obtained using three different nutrient media: Marine Agar, R2A and TCBS. Some of these bacteria were characterized using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and 16S rRNA sequencing. The immune response in larvae exhibiting the "gaping jaws" condition was measured by real time PCR. Our results showed significant differences in bacterial flora between normal and gaping larvae. The gaping yolk sac larvae were predominantly colonized by members of the families Vibrionaceae and Flavobacteriaceae. Bacteria belonging to the Bacillus and Pseudoalteromonas genera were also present but less frequent. It was not possible to associate a type or group of bacteria directly related to "gaping". Strikingly, larvae with gaping jaws had an increase in the expression of two immune related genes, like hepcidin and chemokine (MIP-1B). These results indicate activation of the immune response in larvae with "gaping jaws" syndrome and this response could be related to bacteria isolated from gaping condition.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2014000100007&lng=en&tlng=enhalibut del AtlánticoHippoglossus hippoglossusflora bacterianamandíbula abiertagenes inmunitarios |
| spellingShingle | Rocío Urtubia Pablo Gallardo Paris Lavin Nick Brown Marcelo González Characterization of culturable bacterial flora in yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with "gaping jaws" syndrome Latin American Journal of Aquatic Research halibut del Atlántico Hippoglossus hippoglossus flora bacteriana mandíbula abierta genes inmunitarios |
| title | Characterization of culturable bacterial flora in yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with "gaping jaws" syndrome |
| title_full | Characterization of culturable bacterial flora in yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with "gaping jaws" syndrome |
| title_fullStr | Characterization of culturable bacterial flora in yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with "gaping jaws" syndrome |
| title_full_unstemmed | Characterization of culturable bacterial flora in yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with "gaping jaws" syndrome |
| title_short | Characterization of culturable bacterial flora in yolk-sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with "gaping jaws" syndrome |
| title_sort | characterization of culturable bacterial flora in yolk sac larvae of atlantic halibut hippoglossus hippoglossus l with gaping jaws syndrome |
| topic | halibut del Atlántico Hippoglossus hippoglossus flora bacteriana mandíbula abierta genes inmunitarios |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2014000100007&lng=en&tlng=en |
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