Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line
The study on Erucin (ER) has gained interest of nutraceutical and pharmaceutical industries because of its anti-cancer properties. Erucin is an isothiocyanate obtained from the seeds of Eruca sativa which possess certain drawbacks such as poor aqueous solubility and bioavailability. Therefore, the p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1080977/full |
| _version_ | 1797943604380958720 |
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| author | Sharabjit Singh Gurdeep Singh Shivani Attri Prabhjot Kaur Farhana Rashid Neena Bedi Shafiul Haque Shafiul Haque Essam M. Janahi Saroj Arora |
| author_facet | Sharabjit Singh Gurdeep Singh Shivani Attri Prabhjot Kaur Farhana Rashid Neena Bedi Shafiul Haque Shafiul Haque Essam M. Janahi Saroj Arora |
| author_sort | Sharabjit Singh |
| collection | DOAJ |
| description | The study on Erucin (ER) has gained interest of nutraceutical and pharmaceutical industries because of its anti-cancer properties. Erucin is an isothiocyanate obtained from the seeds of Eruca sativa which possess certain drawbacks such as poor aqueous solubility and bioavailability. Therefore, the present study aimed at developing ER-cubosomes (CUB) by solvent evaporation technique followed by applying Central Composite Design to optimize ER loaded cubosomes. For this purpose, independent variables selected were Monoolein (MO) as lipid and Pluronic-84 (P-84) as a stabilizer whereas dependent variables were particle size, percentage of ER loading and percentage of its entrapment efficiency. The cubosomal nanocarriers exhibited particle size in the range of 26 nm, entrapment efficiency of 99.12 ± 0.04% and drug loading of 3.96 ± 0.0001%. Furthermore, to investigate the antioxidant potential, we checked the effect of ER and ER-CUB by DNA nicking assay, DDPH assay and Phosphomolybdate assay, and results showed significant improvement in antioxidant potential for ER-CUB than ER. Similarly, ER-CUB showed enhanced anticancer activity with a marked reduction in IC50 value than ER in MTT assay. These results suggested that ER-CUB produced notable escalation in antioxidant potential and enhanced anticancer activity than ER. |
| first_indexed | 2024-04-10T20:26:48Z |
| format | Article |
| id | doaj.art-cd399eda5f60487f96cd80d415203814 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-10T20:26:48Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-cd399eda5f60487f96cd80d4152038142023-01-25T10:42:06ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-01-011310.3389/fphar.2022.10809771080977Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell lineSharabjit Singh0Gurdeep Singh1Shivani Attri2Prabhjot Kaur3Farhana Rashid4Neena Bedi5Shafiul Haque6Shafiul Haque7Essam M. Janahi8Saroj Arora9Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, IndiaDepartment of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, IndiaDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, IndiaDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, IndiaDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, IndiaDepartment of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, IndiaResearch and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Janzan, Saudi ArabiaCentre of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab EmiratesIndependent Researcher, Al Janabiyah, BahrainDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, IndiaThe study on Erucin (ER) has gained interest of nutraceutical and pharmaceutical industries because of its anti-cancer properties. Erucin is an isothiocyanate obtained from the seeds of Eruca sativa which possess certain drawbacks such as poor aqueous solubility and bioavailability. Therefore, the present study aimed at developing ER-cubosomes (CUB) by solvent evaporation technique followed by applying Central Composite Design to optimize ER loaded cubosomes. For this purpose, independent variables selected were Monoolein (MO) as lipid and Pluronic-84 (P-84) as a stabilizer whereas dependent variables were particle size, percentage of ER loading and percentage of its entrapment efficiency. The cubosomal nanocarriers exhibited particle size in the range of 26 nm, entrapment efficiency of 99.12 ± 0.04% and drug loading of 3.96 ± 0.0001%. Furthermore, to investigate the antioxidant potential, we checked the effect of ER and ER-CUB by DNA nicking assay, DDPH assay and Phosphomolybdate assay, and results showed significant improvement in antioxidant potential for ER-CUB than ER. Similarly, ER-CUB showed enhanced anticancer activity with a marked reduction in IC50 value than ER in MTT assay. These results suggested that ER-CUB produced notable escalation in antioxidant potential and enhanced anticancer activity than ER.https://www.frontiersin.org/articles/10.3389/fphar.2022.1080977/fullerucincentral composite designcubosomescolon cancerehrlich ascites carcinomaantioxidant assay |
| spellingShingle | Sharabjit Singh Gurdeep Singh Shivani Attri Prabhjot Kaur Farhana Rashid Neena Bedi Shafiul Haque Shafiul Haque Essam M. Janahi Saroj Arora Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line Frontiers in Pharmacology erucin central composite design cubosomes colon cancer ehrlich ascites carcinoma antioxidant assay |
| title | Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line |
| title_full | Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line |
| title_fullStr | Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line |
| title_full_unstemmed | Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line |
| title_short | Development and optimization of nanoparticles loaded with erucin, a dietary isothiocyanate isolated from Eruca sativa: Antioxidant and antiproliferative activities in ehrlich-ascites carcinoma cell line |
| title_sort | development and optimization of nanoparticles loaded with erucin a dietary isothiocyanate isolated from eruca sativa antioxidant and antiproliferative activities in ehrlich ascites carcinoma cell line |
| topic | erucin central composite design cubosomes colon cancer ehrlich ascites carcinoma antioxidant assay |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1080977/full |
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