Effect of lncRNA XIST on acute myeloid leukemia cells via miR-142-5p-PFKP axis
Acute myeloid leukemia (AML) is the common blood cancer in hematopoietic system-related diseases and has a poor prognosis. Studies have shown that long non-coding RNAs (lncRNAs) are closely related to the pathogenesis of a variety of diseases, including AML. However, the specific molecular mechanism...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Hematology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16078454.2024.2306444 |
| _version_ | 1826936801713979392 |
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| author | Zhaozhi Jiang Tingting Liu Youhong Wang Jiao Li Lusheng Guo |
| author_facet | Zhaozhi Jiang Tingting Liu Youhong Wang Jiao Li Lusheng Guo |
| author_sort | Zhaozhi Jiang |
| collection | DOAJ |
| description | Acute myeloid leukemia (AML) is the common blood cancer in hematopoietic system-related diseases and has a poor prognosis. Studies have shown that long non-coding RNAs (lncRNAs) are closely related to the pathogenesis of a variety of diseases, including AML. However, the specific molecular mechanism remains unclear. Hence, the objective of this study was to investigate the effect and mechanism of lncRNA X inactive specific transcript (lncRNA XIST) on AML. To achieve our objective, some tests were performed. Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to detect the expression of lncRNA XIST, miR-142-5p and the platelet isoform of phosphofructokinase (PFKP). The targeting relationship between miR-142-5p and lncRNA XIST and PFKP was verified by Pearson correlation analysis, dual-luciferase reporter assay, and pull-down assay. Functional experiments were used to analyze the effect and mechanism of action of knocking down lncRNA XIST on THP-1 and U937 cells. Compared with bone marrow cells, lncRNA XIST and PFKP expression levels were up-regulated and miR-142-5p expression levels were down-regulated in AML. Further analysis revealed that lncRNA XIST targeted and bound to miR-142-5p, and PFKP was a target gene of miR-142-5p. Knockdown of lncRNA XIST significantly promoted miR-142-5p expression to down-regulate PFKP in THP-1 and U937 cells, while the cell proliferation, cell viability, and cell cycle arrest were inhibited and apoptosis was increased. Knockdown of miR-142-5p reversed the functional impact of lncRNA XIST knockdown on AML cells. In conclusion, down-regulation of lncRNA XIST can affect the progression of AML by regulating miR-142-5p. |
| first_indexed | 2024-03-08T07:58:06Z |
| format | Article |
| id | doaj.art-cd3bfd41a777415b9effef3dd756c968 |
| institution | Directory Open Access Journal |
| issn | 1607-8454 |
| language | English |
| last_indexed | 2025-02-17T18:28:05Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hematology |
| spelling | doaj.art-cd3bfd41a777415b9effef3dd756c9682024-12-12T15:08:53ZengTaylor & Francis GroupHematology1607-84542024-12-0129110.1080/16078454.2024.2306444Effect of lncRNA XIST on acute myeloid leukemia cells via miR-142-5p-PFKP axisZhaozhi Jiang0Tingting Liu1Youhong Wang2Jiao Li3Lusheng Guo4Blood Transfusion Department, Affiliated Hospital of Jilin Medical University, Jilin, ChinaPathology Department, Affiliated Hospital of Jilin Medical University, Jilin, ChinaBlood Transfusion Department, Affiliated Hospital of Jilin Medical University, Jilin, ChinaBlood Transfusion Department, Affiliated Hospital of Jilin Medical University, Jilin, ChinaBlood Transfusion Department, Affiliated Hospital of Jilin Medical University, Jilin, ChinaAcute myeloid leukemia (AML) is the common blood cancer in hematopoietic system-related diseases and has a poor prognosis. Studies have shown that long non-coding RNAs (lncRNAs) are closely related to the pathogenesis of a variety of diseases, including AML. However, the specific molecular mechanism remains unclear. Hence, the objective of this study was to investigate the effect and mechanism of lncRNA X inactive specific transcript (lncRNA XIST) on AML. To achieve our objective, some tests were performed. Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to detect the expression of lncRNA XIST, miR-142-5p and the platelet isoform of phosphofructokinase (PFKP). The targeting relationship between miR-142-5p and lncRNA XIST and PFKP was verified by Pearson correlation analysis, dual-luciferase reporter assay, and pull-down assay. Functional experiments were used to analyze the effect and mechanism of action of knocking down lncRNA XIST on THP-1 and U937 cells. Compared with bone marrow cells, lncRNA XIST and PFKP expression levels were up-regulated and miR-142-5p expression levels were down-regulated in AML. Further analysis revealed that lncRNA XIST targeted and bound to miR-142-5p, and PFKP was a target gene of miR-142-5p. Knockdown of lncRNA XIST significantly promoted miR-142-5p expression to down-regulate PFKP in THP-1 and U937 cells, while the cell proliferation, cell viability, and cell cycle arrest were inhibited and apoptosis was increased. Knockdown of miR-142-5p reversed the functional impact of lncRNA XIST knockdown on AML cells. In conclusion, down-regulation of lncRNA XIST can affect the progression of AML by regulating miR-142-5p.https://www.tandfonline.com/doi/10.1080/16078454.2024.2306444Acute myeloid leukemia (AML)lncRNA X inactive specific transcript (lncRNA XIST)miR-142-5pthe platelet isoform of phosphofructokinase (PFKP)proliferationapoptosis |
| spellingShingle | Zhaozhi Jiang Tingting Liu Youhong Wang Jiao Li Lusheng Guo Effect of lncRNA XIST on acute myeloid leukemia cells via miR-142-5p-PFKP axis Hematology Acute myeloid leukemia (AML) lncRNA X inactive specific transcript (lncRNA XIST) miR-142-5p the platelet isoform of phosphofructokinase (PFKP) proliferation apoptosis |
| title | Effect of lncRNA XIST on acute myeloid leukemia cells via miR-142-5p-PFKP axis |
| title_full | Effect of lncRNA XIST on acute myeloid leukemia cells via miR-142-5p-PFKP axis |
| title_fullStr | Effect of lncRNA XIST on acute myeloid leukemia cells via miR-142-5p-PFKP axis |
| title_full_unstemmed | Effect of lncRNA XIST on acute myeloid leukemia cells via miR-142-5p-PFKP axis |
| title_short | Effect of lncRNA XIST on acute myeloid leukemia cells via miR-142-5p-PFKP axis |
| title_sort | effect of lncrna xist on acute myeloid leukemia cells via mir 142 5p pfkp axis |
| topic | Acute myeloid leukemia (AML) lncRNA X inactive specific transcript (lncRNA XIST) miR-142-5p the platelet isoform of phosphofructokinase (PFKP) proliferation apoptosis |
| url | https://www.tandfonline.com/doi/10.1080/16078454.2024.2306444 |
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