Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells
Our ultimate goal of in vitro derivation of Schwann cells (SCs) from adult bone marrow stromal cells (BMSCs) is such that they may be used autologously to assist post-traumatic nerve regeneration. Existing protocols for derivation of SC-like cells from BMSCs fall short in the stability of the acquir...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671117303612 |
| _version_ | 1811191996393455616 |
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| author | Sa Cai Yat-Ping Tsui Kin-Wai Tam Graham Ka-Hon Shea Richard Shek-Kwan Chang Qiang Ao Daisy Kwok-Yan Shum Ying-Shing Chan |
| author_facet | Sa Cai Yat-Ping Tsui Kin-Wai Tam Graham Ka-Hon Shea Richard Shek-Kwan Chang Qiang Ao Daisy Kwok-Yan Shum Ying-Shing Chan |
| author_sort | Sa Cai |
| collection | DOAJ |
| description | Our ultimate goal of in vitro derivation of Schwann cells (SCs) from adult bone marrow stromal cells (BMSCs) is such that they may be used autologously to assist post-traumatic nerve regeneration. Existing protocols for derivation of SC-like cells from BMSCs fall short in the stability of the acquired phenotype and the functional capacity to myelinate axons. Our experiments indicated that neuro-ectodermal progenitor cells among the human hBMSCs could be selectively expanded and then induced to differentiate into SC-like cells. Co-culture of the SC-like cells with embryonic dorsal root ganglion neurons facilitated contact-mediated signaling that accomplished the switch to fate-committed SCs. Microarray analysis and in vitro myelination provided evidence that the human BMSC-derived SCs were functionally mature. This was reinforced by repair and myelination phenotypes observable in vivo with the derived SCs seeded into a nerve guide as an implant across a critical gap in a rat model of sciatic nerve injury. |
| first_indexed | 2024-04-11T23:45:54Z |
| format | Article |
| id | doaj.art-cd45b472256c4da7aaa538e09718c530 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-11T23:45:54Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-cd45b472256c4da7aaa538e09718c5302022-12-22T03:56:39ZengElsevierStem Cell Reports2213-67112017-10-01941097110810.1016/j.stemcr.2017.08.004Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann CellsSa Cai0Yat-Ping Tsui1Kin-Wai Tam2Graham Ka-Hon Shea3Richard Shek-Kwan Chang4Qiang Ao5Daisy Kwok-Yan Shum6Ying-Shing Chan7School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SARSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SARSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SARDepartment of Orthopaedics & Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SARDepartment of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SARDepartment of Tissue Engineering, China Medical University, Shenyang, PR ChinaSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SARSchool of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAROur ultimate goal of in vitro derivation of Schwann cells (SCs) from adult bone marrow stromal cells (BMSCs) is such that they may be used autologously to assist post-traumatic nerve regeneration. Existing protocols for derivation of SC-like cells from BMSCs fall short in the stability of the acquired phenotype and the functional capacity to myelinate axons. Our experiments indicated that neuro-ectodermal progenitor cells among the human hBMSCs could be selectively expanded and then induced to differentiate into SC-like cells. Co-culture of the SC-like cells with embryonic dorsal root ganglion neurons facilitated contact-mediated signaling that accomplished the switch to fate-committed SCs. Microarray analysis and in vitro myelination provided evidence that the human BMSC-derived SCs were functionally mature. This was reinforced by repair and myelination phenotypes observable in vivo with the derived SCs seeded into a nerve guide as an implant across a critical gap in a rat model of sciatic nerve injury.http://www.sciencedirect.com/science/article/pii/S2213671117303612bone marrow stromal cellSchwann celldifferentiationfate commitmentmyelination |
| spellingShingle | Sa Cai Yat-Ping Tsui Kin-Wai Tam Graham Ka-Hon Shea Richard Shek-Kwan Chang Qiang Ao Daisy Kwok-Yan Shum Ying-Shing Chan Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells Stem Cell Reports bone marrow stromal cell Schwann cell differentiation fate commitment myelination |
| title | Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells |
| title_full | Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells |
| title_fullStr | Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells |
| title_full_unstemmed | Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells |
| title_short | Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells |
| title_sort | directed differentiation of human bone marrow stromal cells to fate committed schwann cells |
| topic | bone marrow stromal cell Schwann cell differentiation fate commitment myelination |
| url | http://www.sciencedirect.com/science/article/pii/S2213671117303612 |
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