Autophagy Flux Contributes to Regulation of Components of Eclipta prostrata L. on Cigarette Smoking-Induced Injury of Bronchial Epithelial Cells
Excessive autophagy plays a crucial role in cigarette smoking extract (CSE)-induced inflammation response and oxidative damage of respiratory epithelial cells. The components from Eclipta prostrata L. (CCE) have been shown to be beneficial for CSE-induced epithelial cells injury. However, whether it...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2018.00107/full |
| _version_ | 1811191996600025088 |
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| author | Shumin Ding Xuefeng Hou Xuefeng Hou Gang Wang Gang Wang Huihui Qiu Ying Liu Ying Liu Yuanli Zhou Mei Du Mei Du Xiaobin Tan Jie Song Yingjie Wei Luan Shu Zhiyong Li Liang Feng Liang Feng Xiaobin Jia Xiaobin Jia Xiaobin Jia |
| author_facet | Shumin Ding Xuefeng Hou Xuefeng Hou Gang Wang Gang Wang Huihui Qiu Ying Liu Ying Liu Yuanli Zhou Mei Du Mei Du Xiaobin Tan Jie Song Yingjie Wei Luan Shu Zhiyong Li Liang Feng Liang Feng Xiaobin Jia Xiaobin Jia Xiaobin Jia |
| author_sort | Shumin Ding |
| collection | DOAJ |
| description | Excessive autophagy plays a crucial role in cigarette smoking extract (CSE)-induced inflammation response and oxidative damage of respiratory epithelial cells. The components from Eclipta prostrata L. (CCE) have been shown to be beneficial for CSE-induced epithelial cells injury. However, whether its protection on CSE-stress injury is related to its regulation on autophagy remains still unclear. In this study, CCE, containing mainly wedelolactone of 45.88% and demethylwedelolactone of 23.74%, could improve significantly 10%CSE-induced cell viability of normal human bronchial epithelial (NHBE) cells using CCK-8 kit. We revealed that CCE could remarkably increase autophagic factors Beclin-1, Atg5, ATF4 proteins expression levels and the transformation of LC3-I to LC3-II. Additionally, CCE up-regulated significantly p-p16 and p-p21 phosphorylation levels whereas down-regulated p-p53 in NHBE cells. The changes of typical autolysosom and representative autophagosome in the presence of CCE or/and autophagy inhibitor chloroquine (CQ) were also observed by transmission electron microscopy. These data demonstrated that CCE reduced CSE-induced autophagy flux activation in NHBE cells. The blockade of CCE on autophagy flux contributes to its protection against CSE-induced NHBE cells damage, and CCE is promising to be combination therapeutic molecules to excessive autophagic damage in respiratory diseases. |
| first_indexed | 2024-04-11T23:45:54Z |
| format | Article |
| id | doaj.art-cd46f425ecb84e02ae5d49dc754afb71 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T23:45:54Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-cd46f425ecb84e02ae5d49dc754afb712022-12-22T03:56:39ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-02-01910.3389/fphar.2018.00107290439Autophagy Flux Contributes to Regulation of Components of Eclipta prostrata L. on Cigarette Smoking-Induced Injury of Bronchial Epithelial CellsShumin Ding0Xuefeng Hou1Xuefeng Hou2Gang Wang3Gang Wang4Huihui Qiu5Ying Liu6Ying Liu7Yuanli Zhou8Mei Du9Mei Du10Xiaobin Tan11Jie Song12Yingjie Wei13Luan Shu14Zhiyong Li15Liang Feng16Liang Feng17Xiaobin Jia18Xiaobin Jia19Xiaobin Jia20School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaCollege of Traditional Chinese Medicine, Anhui University of Chinese Medicine, Hefei, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaCollege of Traditional Chinese Medicine, Anhui University of Chinese Medicine, Hefei, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaCollege of Traditional Chinese Medicine, Anhui University of Chinese Medicine, Hefei, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaCollege of Traditional Chinese Medicine, Anhui University of Chinese Medicine, Hefei, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaChina Minority Traditional Medical Center, Minzu University of China, Beijing, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaCollege of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, ChinaKey Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, ChinaCollege of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, ChinaAffliated Hospital of Long Hua, Shanghai University of Chinese Medicine, Shanghai, ChinaExcessive autophagy plays a crucial role in cigarette smoking extract (CSE)-induced inflammation response and oxidative damage of respiratory epithelial cells. The components from Eclipta prostrata L. (CCE) have been shown to be beneficial for CSE-induced epithelial cells injury. However, whether its protection on CSE-stress injury is related to its regulation on autophagy remains still unclear. In this study, CCE, containing mainly wedelolactone of 45.88% and demethylwedelolactone of 23.74%, could improve significantly 10%CSE-induced cell viability of normal human bronchial epithelial (NHBE) cells using CCK-8 kit. We revealed that CCE could remarkably increase autophagic factors Beclin-1, Atg5, ATF4 proteins expression levels and the transformation of LC3-I to LC3-II. Additionally, CCE up-regulated significantly p-p16 and p-p21 phosphorylation levels whereas down-regulated p-p53 in NHBE cells. The changes of typical autolysosom and representative autophagosome in the presence of CCE or/and autophagy inhibitor chloroquine (CQ) were also observed by transmission electron microscopy. These data demonstrated that CCE reduced CSE-induced autophagy flux activation in NHBE cells. The blockade of CCE on autophagy flux contributes to its protection against CSE-induced NHBE cells damage, and CCE is promising to be combination therapeutic molecules to excessive autophagic damage in respiratory diseases.http://journal.frontiersin.org/article/10.3389/fphar.2018.00107/fullEclipta prostrata L.autophagycigarette smokingstress injurybronchial epithelial cells |
| spellingShingle | Shumin Ding Xuefeng Hou Xuefeng Hou Gang Wang Gang Wang Huihui Qiu Ying Liu Ying Liu Yuanli Zhou Mei Du Mei Du Xiaobin Tan Jie Song Yingjie Wei Luan Shu Zhiyong Li Liang Feng Liang Feng Xiaobin Jia Xiaobin Jia Xiaobin Jia Autophagy Flux Contributes to Regulation of Components of Eclipta prostrata L. on Cigarette Smoking-Induced Injury of Bronchial Epithelial Cells Frontiers in Pharmacology Eclipta prostrata L. autophagy cigarette smoking stress injury bronchial epithelial cells |
| title | Autophagy Flux Contributes to Regulation of Components of Eclipta prostrata L. on Cigarette Smoking-Induced Injury of Bronchial Epithelial Cells |
| title_full | Autophagy Flux Contributes to Regulation of Components of Eclipta prostrata L. on Cigarette Smoking-Induced Injury of Bronchial Epithelial Cells |
| title_fullStr | Autophagy Flux Contributes to Regulation of Components of Eclipta prostrata L. on Cigarette Smoking-Induced Injury of Bronchial Epithelial Cells |
| title_full_unstemmed | Autophagy Flux Contributes to Regulation of Components of Eclipta prostrata L. on Cigarette Smoking-Induced Injury of Bronchial Epithelial Cells |
| title_short | Autophagy Flux Contributes to Regulation of Components of Eclipta prostrata L. on Cigarette Smoking-Induced Injury of Bronchial Epithelial Cells |
| title_sort | autophagy flux contributes to regulation of components of eclipta prostrata l on cigarette smoking induced injury of bronchial epithelial cells |
| topic | Eclipta prostrata L. autophagy cigarette smoking stress injury bronchial epithelial cells |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2018.00107/full |
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