The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites
Microtubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored. CAMSAP homologs associate with microtubule minus-ends, and are important for the stability of epithelial cell adhesions. In this study, we rep...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2014-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/01637 |
| _version_ | 1818018951843545088 |
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| author | Jana Dorfman Marcette Jessica Jie Chen Michael L Nonet |
| author_facet | Jana Dorfman Marcette Jessica Jie Chen Michael L Nonet |
| author_sort | Jana Dorfman Marcette |
| collection | DOAJ |
| description | Microtubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored. CAMSAP homologs associate with microtubule minus-ends, and are important for the stability of epithelial cell adhesions. In this study, we report morphological defects in neurons and neuromuscular defects in mutants of the C. elegans CAMSAP, ptrn-1. Mechanosensory neurons initially extend wild-type neurites, and subsequently remodel by overextending neurites and retracting synaptic branches and presynaptic varicosities. This neuronal remodeling can be activated by mutations known to alter microtubules, and depends on a functioning DLK-1 MAP kinase pathway. We found that PTRN-1 localizes to both neurites and synapses, and our results suggest that alterations of microtubule structures caused by loss of PTRN-1 function activates a remodeling program leading to changes in neurite morphology. We propose a model whereby minus-end microtubule stabilization mediated by a functional PTRN-1 is necessary for morphological maintenance of neurons. |
| first_indexed | 2024-04-14T07:46:31Z |
| format | Article |
| id | doaj.art-cd48dda5a2364b238df398c5d218c5ab |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:46:31Z |
| publishDate | 2014-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-cd48dda5a2364b238df398c5d218c5ab2022-12-22T02:05:20ZengeLife Sciences Publications LtdeLife2050-084X2014-02-01310.7554/eLife.01637The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neuritesJana Dorfman Marcette0Jessica Jie Chen1Michael L Nonet2Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, United StatesDepartment of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, United StatesDepartment of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, United StatesMicrotubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored. CAMSAP homologs associate with microtubule minus-ends, and are important for the stability of epithelial cell adhesions. In this study, we report morphological defects in neurons and neuromuscular defects in mutants of the C. elegans CAMSAP, ptrn-1. Mechanosensory neurons initially extend wild-type neurites, and subsequently remodel by overextending neurites and retracting synaptic branches and presynaptic varicosities. This neuronal remodeling can be activated by mutations known to alter microtubules, and depends on a functioning DLK-1 MAP kinase pathway. We found that PTRN-1 localizes to both neurites and synapses, and our results suggest that alterations of microtubule structures caused by loss of PTRN-1 function activates a remodeling program leading to changes in neurite morphology. We propose a model whereby minus-end microtubule stabilization mediated by a functional PTRN-1 is necessary for morphological maintenance of neurons.https://elifesciences.org/articles/01637PTRN-1microtubule minus-endneuron morphologyregenerationDLK-1 |
| spellingShingle | Jana Dorfman Marcette Jessica Jie Chen Michael L Nonet The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites eLife PTRN-1 microtubule minus-end neuron morphology regeneration DLK-1 |
| title | The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites |
| title_full | The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites |
| title_fullStr | The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites |
| title_full_unstemmed | The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites |
| title_short | The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites |
| title_sort | caenorhabditis elegans microtubule minus end binding homolog ptrn 1 stabilizes synapses and neurites |
| topic | PTRN-1 microtubule minus-end neuron morphology regeneration DLK-1 |
| url | https://elifesciences.org/articles/01637 |
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