Raman Spectroscopy of Individual Cervical Exfoliated Cells in Premalignant and Malignant Lesions
Cervical cancer is frequent neoplasia. Currently, the diagnostic approach includes cervical cytology, colposcopy, and histopathology studies; combining detection techniques increases the sensitivity and specificity of the tests. Raman spectroscopy is a high-resolution technique that supports the dia...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/5/2419 |
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| author | Mariana Sarai Silva-López César Arturo Ilizaliturri Hernández Hugo Ricardo Navarro Contreras Ángel Gabriel Rodríguez Vázquez Alejandra Ortiz-Dosal Eleazar Samuel Kolosovas-Machuca |
| author_facet | Mariana Sarai Silva-López César Arturo Ilizaliturri Hernández Hugo Ricardo Navarro Contreras Ángel Gabriel Rodríguez Vázquez Alejandra Ortiz-Dosal Eleazar Samuel Kolosovas-Machuca |
| author_sort | Mariana Sarai Silva-López |
| collection | DOAJ |
| description | Cervical cancer is frequent neoplasia. Currently, the diagnostic approach includes cervical cytology, colposcopy, and histopathology studies; combining detection techniques increases the sensitivity and specificity of the tests. Raman spectroscopy is a high-resolution technique that supports the diagnosis of malignancies. This study aimed to evaluate the Raman spectroscopy technique discriminating between healthy and premalignant/malignant cervical cells. We included 81 exfoliative cytology samples, 29 in the “healthy group” (negative cytology), and 52 in the “CIN group” (premalignant/malignant lesions). We obtained the nucleus and cytoplasm Raman spectra of individual cells. We tested the spectral differences between groups using Permutational Multivariate Analysis of Variance (PERMANOVA) and Canonical Analysis of Principal Coordinates (CAP). We found that Raman spectra have increased intensity in premalignant/malignant cells compared with healthy cells. The characteristic Raman bands corresponded to proteins and nucleic acids, in concordance with the increased replication and translation processes in premalignant/malignant states. We found a classification efficiency of 76.5% and 82.7% for cytoplasmic and nuclear Raman spectra, respectively; cell nucleus Raman spectra showed a sensitivity of 84.6% in identifying cervical anomalies. The classification efficiency and sensitivity obtained for nuclear spectra suggest that Raman spectroscopy could be helpful in the screening and diagnosis of premalignant lesions and cervical cancer. |
| first_indexed | 2024-03-09T20:48:56Z |
| format | Article |
| id | doaj.art-cd48fca9784f490ebdd04408cd8aa452 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T20:48:56Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-cd48fca9784f490ebdd04408cd8aa4522023-11-23T22:40:28ZengMDPI AGApplied Sciences2076-34172022-02-01125241910.3390/app12052419Raman Spectroscopy of Individual Cervical Exfoliated Cells in Premalignant and Malignant LesionsMariana Sarai Silva-López0César Arturo Ilizaliturri Hernández1Hugo Ricardo Navarro Contreras2Ángel Gabriel Rodríguez Vázquez3Alejandra Ortiz-Dosal4Eleazar Samuel Kolosovas-Machuca5Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, 550-2a Sierra Leona Ave, San Luis Potosí 78210, MexicoCIACYT-Facultad de Medicina, Universidad Autónoma de San Luís Potosí, 550-2a Sierra Leona Ave, San Luis Potosí 78210, MexicoCoordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, 550-2a Sierra Leona Ave, San Luis Potosí 78210, MexicoCoordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, 550-2a Sierra Leona Ave, San Luis Potosí 78210, MexicoDoctorado Institucional en Ingeniería y Ciencia de Materiales (DICIM-UASLP), Universidad Autónoma de San Luis Potosí, 550 Sierra Leona Ave, San Luis Potosí 78210, MexicoCoordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, 550-2a Sierra Leona Ave, San Luis Potosí 78210, MexicoCervical cancer is frequent neoplasia. Currently, the diagnostic approach includes cervical cytology, colposcopy, and histopathology studies; combining detection techniques increases the sensitivity and specificity of the tests. Raman spectroscopy is a high-resolution technique that supports the diagnosis of malignancies. This study aimed to evaluate the Raman spectroscopy technique discriminating between healthy and premalignant/malignant cervical cells. We included 81 exfoliative cytology samples, 29 in the “healthy group” (negative cytology), and 52 in the “CIN group” (premalignant/malignant lesions). We obtained the nucleus and cytoplasm Raman spectra of individual cells. We tested the spectral differences between groups using Permutational Multivariate Analysis of Variance (PERMANOVA) and Canonical Analysis of Principal Coordinates (CAP). We found that Raman spectra have increased intensity in premalignant/malignant cells compared with healthy cells. The characteristic Raman bands corresponded to proteins and nucleic acids, in concordance with the increased replication and translation processes in premalignant/malignant states. We found a classification efficiency of 76.5% and 82.7% for cytoplasmic and nuclear Raman spectra, respectively; cell nucleus Raman spectra showed a sensitivity of 84.6% in identifying cervical anomalies. The classification efficiency and sensitivity obtained for nuclear spectra suggest that Raman spectroscopy could be helpful in the screening and diagnosis of premalignant lesions and cervical cancer.https://www.mdpi.com/2076-3417/12/5/2419cancer screeningcervical cytologysingle-cell Raman spectroscopycervical intraepithelial neoplasiamalignant cervical lesions |
| spellingShingle | Mariana Sarai Silva-López César Arturo Ilizaliturri Hernández Hugo Ricardo Navarro Contreras Ángel Gabriel Rodríguez Vázquez Alejandra Ortiz-Dosal Eleazar Samuel Kolosovas-Machuca Raman Spectroscopy of Individual Cervical Exfoliated Cells in Premalignant and Malignant Lesions Applied Sciences cancer screening cervical cytology single-cell Raman spectroscopy cervical intraepithelial neoplasia malignant cervical lesions |
| title | Raman Spectroscopy of Individual Cervical Exfoliated Cells in Premalignant and Malignant Lesions |
| title_full | Raman Spectroscopy of Individual Cervical Exfoliated Cells in Premalignant and Malignant Lesions |
| title_fullStr | Raman Spectroscopy of Individual Cervical Exfoliated Cells in Premalignant and Malignant Lesions |
| title_full_unstemmed | Raman Spectroscopy of Individual Cervical Exfoliated Cells in Premalignant and Malignant Lesions |
| title_short | Raman Spectroscopy of Individual Cervical Exfoliated Cells in Premalignant and Malignant Lesions |
| title_sort | raman spectroscopy of individual cervical exfoliated cells in premalignant and malignant lesions |
| topic | cancer screening cervical cytology single-cell Raman spectroscopy cervical intraepithelial neoplasia malignant cervical lesions |
| url | https://www.mdpi.com/2076-3417/12/5/2419 |
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