BMP-2 signaling in ovarian cancer and its association with poor prognosis
<p>Abstract</p> <p>Background</p> <p>We previously observed the over-expression of BMP-2 in primary cultures of epithelial ovarian cancer (EOC) cells as compared to normal epithelial cells based on Affymetrix microarray profiling <abbrgrp><abbr bid="B1&quo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-04-01
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| Series: | Journal of Ovarian Research |
| Online Access: | http://www.ovarianresearch.com/content/2/1/4 |
| _version_ | 1797968306715492352 |
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| author | Le Page Cécile Puiffe Marie-Line Meunier Liliane Zietarska Magdalena de Ladurantaye Manon Tonin Patricia N Provencher Diane Mes-Masson Anne-Marie |
| author_facet | Le Page Cécile Puiffe Marie-Line Meunier Liliane Zietarska Magdalena de Ladurantaye Manon Tonin Patricia N Provencher Diane Mes-Masson Anne-Marie |
| author_sort | Le Page Cécile |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>We previously observed the over-expression of BMP-2 in primary cultures of epithelial ovarian cancer (EOC) cells as compared to normal epithelial cells based on Affymetrix microarray profiling <abbrgrp><abbr bid="B1">1</abbr></abbrgrp>. Here we investigate the effect of BMP-2 on several parameters of ovarian cancer tumorigenesis using the TOV-2223, TOV-1946 and TOV-112D EOC cell lines.</p> <p>Methods</p> <p>We treated each EOC cell line with recombinant BMP-2 and assayed various parameters associated with tumorigenesis. More specifically, cell signaling events induced by BMP-2 treatment were investigated by western-blot using anti-phosphospecific antibodies. Induction of <it>Id1, Snail </it>and <it>Smad6 </it>mRNA expression was investigated by real time RT-PCR. The ability of cells to migrate was tested using the scratch assay. Cell-cell adhesion was analyzed by the ability of cells to form spheroids. We also investigated BMP-2 expression in tissue samples from a series of EOC patients.</p> <p>Results</p> <p>Treatment of these cell lines with recombinant BMP-2 induced a rapid phosphorylation of Smad1/5/8 and Erk MAPKs. Increased expression of <it>Id1</it>, <it>Smad6 </it>and <it>Snail </it>mRNAs was also observed. Only in the TOV-2223 cell line were these signaling events accompanied by an alteration in cell proliferation. We also observed that BMP-2 efficiently increased the motility of all three cell lines. In contrast, BMP-2 treatment decreased the ability of TOV-1946 and TOV-112D cell lines to form spheroids indicating an inhibition of cell-cell adhesion. The expression of BMP-2 in tumor tissues from patients was inversely correlated with survival.</p> <p>Conclusion</p> <p>These results suggest that EOC cell secretion of BMP-2 in the tumor environment contributes to a modification of tumor cell behavior through a change in motility and adherence. We also show that BMP-2 expression in tumor tissues is associated with a poorer prognosis for ovarian cancer patients.</p> |
| first_indexed | 2024-04-11T02:44:48Z |
| format | Article |
| id | doaj.art-cd4a1128e9814b44954c23f6c7e598a6 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T02:44:48Z |
| publishDate | 2009-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-cd4a1128e9814b44954c23f6c7e598a62023-01-02T18:15:35ZengBMCJournal of Ovarian Research1757-22152009-04-0121410.1186/1757-2215-2-4BMP-2 signaling in ovarian cancer and its association with poor prognosisLe Page CécilePuiffe Marie-LineMeunier LilianeZietarska Magdalenade Ladurantaye ManonTonin Patricia NProvencher DianeMes-Masson Anne-Marie<p>Abstract</p> <p>Background</p> <p>We previously observed the over-expression of BMP-2 in primary cultures of epithelial ovarian cancer (EOC) cells as compared to normal epithelial cells based on Affymetrix microarray profiling <abbrgrp><abbr bid="B1">1</abbr></abbrgrp>. Here we investigate the effect of BMP-2 on several parameters of ovarian cancer tumorigenesis using the TOV-2223, TOV-1946 and TOV-112D EOC cell lines.</p> <p>Methods</p> <p>We treated each EOC cell line with recombinant BMP-2 and assayed various parameters associated with tumorigenesis. More specifically, cell signaling events induced by BMP-2 treatment were investigated by western-blot using anti-phosphospecific antibodies. Induction of <it>Id1, Snail </it>and <it>Smad6 </it>mRNA expression was investigated by real time RT-PCR. The ability of cells to migrate was tested using the scratch assay. Cell-cell adhesion was analyzed by the ability of cells to form spheroids. We also investigated BMP-2 expression in tissue samples from a series of EOC patients.</p> <p>Results</p> <p>Treatment of these cell lines with recombinant BMP-2 induced a rapid phosphorylation of Smad1/5/8 and Erk MAPKs. Increased expression of <it>Id1</it>, <it>Smad6 </it>and <it>Snail </it>mRNAs was also observed. Only in the TOV-2223 cell line were these signaling events accompanied by an alteration in cell proliferation. We also observed that BMP-2 efficiently increased the motility of all three cell lines. In contrast, BMP-2 treatment decreased the ability of TOV-1946 and TOV-112D cell lines to form spheroids indicating an inhibition of cell-cell adhesion. The expression of BMP-2 in tumor tissues from patients was inversely correlated with survival.</p> <p>Conclusion</p> <p>These results suggest that EOC cell secretion of BMP-2 in the tumor environment contributes to a modification of tumor cell behavior through a change in motility and adherence. We also show that BMP-2 expression in tumor tissues is associated with a poorer prognosis for ovarian cancer patients.</p>http://www.ovarianresearch.com/content/2/1/4 |
| spellingShingle | Le Page Cécile Puiffe Marie-Line Meunier Liliane Zietarska Magdalena de Ladurantaye Manon Tonin Patricia N Provencher Diane Mes-Masson Anne-Marie BMP-2 signaling in ovarian cancer and its association with poor prognosis Journal of Ovarian Research |
| title | BMP-2 signaling in ovarian cancer and its association with poor prognosis |
| title_full | BMP-2 signaling in ovarian cancer and its association with poor prognosis |
| title_fullStr | BMP-2 signaling in ovarian cancer and its association with poor prognosis |
| title_full_unstemmed | BMP-2 signaling in ovarian cancer and its association with poor prognosis |
| title_short | BMP-2 signaling in ovarian cancer and its association with poor prognosis |
| title_sort | bmp 2 signaling in ovarian cancer and its association with poor prognosis |
| url | http://www.ovarianresearch.com/content/2/1/4 |
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