The Effects of Seed Pretreatment with Endophytic Bacteria <i>Bacillus subtilis</i> on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit
We investigated the effect of pre-sowing seed treatment with endophytic <i>Bacillus subtilis</i> 10-4 (<i>B. subtilis</i>) on spring and winter wheat (<i>Triticum aestivum</i> L.; cultivars Ekada-70 (Ek) and Scepter (Sc), respectively) growth and tolerance under 1...
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MDPI AG
2023-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/14/2684 |
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| author | Alsu R. Lubyanova Chulpan R. Allagulova Oksana V. Lastochkina |
| author_facet | Alsu R. Lubyanova Chulpan R. Allagulova Oksana V. Lastochkina |
| author_sort | Alsu R. Lubyanova |
| collection | DOAJ |
| description | We investigated the effect of pre-sowing seed treatment with endophytic <i>Bacillus subtilis</i> 10-4 (<i>B. subtilis</i>) on spring and winter wheat (<i>Triticum aestivum</i> L.; cultivars Ekada-70 (Ek) and Scepter (Sc), respectively) growth and tolerance under 1–24 h of drought stress, modulated by 12% polyethylene glycol 6000 (PEG). The results showed that drought decreased transpiration intensity (TI), root relative water content (RWC), osmotic potential (Ψ<sub>π</sub>) of cell sap, and induced proline accumulation and electrolyte leakage (EL) in both wheat cultivars. It was revealed that Sc was more responsive to PEG and <i>B. subtilis</i> treatments than Ek. Under drought, Ek did not significantly change root length, shoot height, or dry biomass. The pretreatment of wheat plants with <i>B. subtilis</i> performed significantly better under drought conditions through the enhanced TI, RWC, and Ψ<sub>π</sub> of the cell sap in comparison with the plants treated with 12% PEG alone. <i>B. subtilis</i> also reduced stress-caused EL, especially in the Sc cultivar. Under water deficit wheat seedlings, pretreated with <i>B. subtilis</i>, have a higher proline accumulation in comparison to untreated stressed plants. Taken together, our results demonstrate the crucial role of endophytic <i>B. subtilis</i> in ameliorating the adverse effects of water stress on the water balance of both winter and spring wheat cultivars. |
| first_indexed | 2024-03-11T00:43:21Z |
| format | Article |
| id | doaj.art-cd4aa1caf34c4b209c4f7e75c3af531a |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T00:43:21Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-cd4aa1caf34c4b209c4f7e75c3af531a2023-11-18T21:00:33ZengMDPI AGPlants2223-77472023-07-011214268410.3390/plants12142684The Effects of Seed Pretreatment with Endophytic Bacteria <i>Bacillus subtilis</i> on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water DeficitAlsu R. Lubyanova0Chulpan R. Allagulova1Oksana V. Lastochkina2Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Prospect Oktyabrya 71, 450054 Ufa, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Prospect Oktyabrya 71, 450054 Ufa, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Prospect Oktyabrya 71, 450054 Ufa, RussiaWe investigated the effect of pre-sowing seed treatment with endophytic <i>Bacillus subtilis</i> 10-4 (<i>B. subtilis</i>) on spring and winter wheat (<i>Triticum aestivum</i> L.; cultivars Ekada-70 (Ek) and Scepter (Sc), respectively) growth and tolerance under 1–24 h of drought stress, modulated by 12% polyethylene glycol 6000 (PEG). The results showed that drought decreased transpiration intensity (TI), root relative water content (RWC), osmotic potential (Ψ<sub>π</sub>) of cell sap, and induced proline accumulation and electrolyte leakage (EL) in both wheat cultivars. It was revealed that Sc was more responsive to PEG and <i>B. subtilis</i> treatments than Ek. Under drought, Ek did not significantly change root length, shoot height, or dry biomass. The pretreatment of wheat plants with <i>B. subtilis</i> performed significantly better under drought conditions through the enhanced TI, RWC, and Ψ<sub>π</sub> of the cell sap in comparison with the plants treated with 12% PEG alone. <i>B. subtilis</i> also reduced stress-caused EL, especially in the Sc cultivar. Under water deficit wheat seedlings, pretreated with <i>B. subtilis</i>, have a higher proline accumulation in comparison to untreated stressed plants. Taken together, our results demonstrate the crucial role of endophytic <i>B. subtilis</i> in ameliorating the adverse effects of water stress on the water balance of both winter and spring wheat cultivars.https://www.mdpi.com/2223-7747/12/14/2684winter and spring <i>Triticum aestivum</i> L.drought stressendophytic PGP bacteriaseed bioprimingosmotic potentialproline |
| spellingShingle | Alsu R. Lubyanova Chulpan R. Allagulova Oksana V. Lastochkina The Effects of Seed Pretreatment with Endophytic Bacteria <i>Bacillus subtilis</i> on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit Plants winter and spring <i>Triticum aestivum</i> L. drought stress endophytic PGP bacteria seed biopriming osmotic potential proline |
| title | The Effects of Seed Pretreatment with Endophytic Bacteria <i>Bacillus subtilis</i> on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit |
| title_full | The Effects of Seed Pretreatment with Endophytic Bacteria <i>Bacillus subtilis</i> on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit |
| title_fullStr | The Effects of Seed Pretreatment with Endophytic Bacteria <i>Bacillus subtilis</i> on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit |
| title_full_unstemmed | The Effects of Seed Pretreatment with Endophytic Bacteria <i>Bacillus subtilis</i> on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit |
| title_short | The Effects of Seed Pretreatment with Endophytic Bacteria <i>Bacillus subtilis</i> on the Water Balance of Spring and Winter Wheat Seedlings under Short-Time Water Deficit |
| title_sort | effects of seed pretreatment with endophytic bacteria i bacillus subtilis i on the water balance of spring and winter wheat seedlings under short time water deficit |
| topic | winter and spring <i>Triticum aestivum</i> L. drought stress endophytic PGP bacteria seed biopriming osmotic potential proline |
| url | https://www.mdpi.com/2223-7747/12/14/2684 |
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