A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4+-N removal in shrimp aquaculture water
Photosynthetic bacteria (PSB) attract considerable interest as useful microorganisms; nevertheless, a generalized culture technique has not been previously reported owing to difficulty in their cultivation. Therefore, a simple culture technique suitable for public use was investigated. Among the PSB...
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| Format: | Article |
| Language: | English |
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The Korean Society of Fisheries and Aquatic Science
2022-10-01
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| Series: | Fisheries and Aquatic Sciences |
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| Online Access: | http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2022.e47 |
| _version_ | 1811248710492880896 |
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| author | Kyoung Sook Cho Joong Kyun Kim |
| author_facet | Kyoung Sook Cho Joong Kyun Kim |
| author_sort | Kyoung Sook Cho |
| collection | DOAJ |
| description | Photosynthetic bacteria (PSB) attract considerable interest as useful microorganisms; nevertheless, a generalized culture technique has not been previously reported owing to difficulty in their cultivation. Therefore, a simple culture technique suitable for public use was investigated. Among the PSB tested, the strain Rhodobacter azotoformans EBN-7 was the most suitable for scaleup production because it showed the highest specific growth rate (0.20 h−1) on basal medium. In scale-up cultivation (500 L), R. azotoformans EBN-7 showed 4.50 × 1010 colony-forming units mL−1 (number of viable cells), dry cell weight of 26.8 g/L, and a specific growth rate of 0.15 h−1. Cultivation using this final culture broth (as seed culture) in a 15 L simple reactor was successful, with maintenance of cell activity evident. For use as seed culture, the maximum allowable preservation period of R. azotoformans EBN-7 at 4°C was 3 months. When R. azotoformans EBN-7 cultivated in a simple technique was applied to shrimp aquaculture water, NH4+-N was reduced from 0.61 mg/L to 0.24 mg/L (by 60.7%) in 4 days in comparison with the control. Thus, this simple culture technique using R. azotoformans EBN-7 has the potential for a good removal efficiency of NH4+-N, making seed culture easier and suitable for public use. |
| first_indexed | 2024-04-12T15:32:47Z |
| format | Article |
| id | doaj.art-cd4b63c0548c4b1287da195430c149c9 |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-04-12T15:32:47Z |
| publishDate | 2022-10-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-cd4b63c0548c4b1287da195430c149c92022-12-22T03:27:02ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572022-10-01251052553610.47853/FAS.2022.e47A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4+-N removal in shrimp aquaculture waterKyoung Sook Cho0Joong Kyun Kim1Department of Bioengineering, Pukyong National University, Busan 48513, KoreaDepartment of Bioengineering, Pukyong National University, Busan 48513, KoreaPhotosynthetic bacteria (PSB) attract considerable interest as useful microorganisms; nevertheless, a generalized culture technique has not been previously reported owing to difficulty in their cultivation. Therefore, a simple culture technique suitable for public use was investigated. Among the PSB tested, the strain Rhodobacter azotoformans EBN-7 was the most suitable for scaleup production because it showed the highest specific growth rate (0.20 h−1) on basal medium. In scale-up cultivation (500 L), R. azotoformans EBN-7 showed 4.50 × 1010 colony-forming units mL−1 (number of viable cells), dry cell weight of 26.8 g/L, and a specific growth rate of 0.15 h−1. Cultivation using this final culture broth (as seed culture) in a 15 L simple reactor was successful, with maintenance of cell activity evident. For use as seed culture, the maximum allowable preservation period of R. azotoformans EBN-7 at 4°C was 3 months. When R. azotoformans EBN-7 cultivated in a simple technique was applied to shrimp aquaculture water, NH4+-N was reduced from 0.61 mg/L to 0.24 mg/L (by 60.7%) in 4 days in comparison with the control. Thus, this simple culture technique using R. azotoformans EBN-7 has the potential for a good removal efficiency of NH4+-N, making seed culture easier and suitable for public use. http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2022.e47Rhodobacter azotoformans EBN-7Seed cultureSimple culture techniqueScale-up productionShrimp aquaculture water |
| spellingShingle | Kyoung Sook Cho Joong Kyun Kim A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4+-N removal in shrimp aquaculture water Fisheries and Aquatic Sciences Rhodobacter azotoformans EBN-7 Seed culture Simple culture technique Scale-up production Shrimp aquaculture water |
| title | A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4+-N removal in shrimp aquaculture water |
| title_full | A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4+-N removal in shrimp aquaculture water |
| title_fullStr | A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4+-N removal in shrimp aquaculture water |
| title_full_unstemmed | A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4+-N removal in shrimp aquaculture water |
| title_short | A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4+-N removal in shrimp aquaculture water |
| title_sort | simple culture technique of rhodobacter azotoformans ebn 7 for public use application to nh4 n removal in shrimp aquaculture water |
| topic | Rhodobacter azotoformans EBN-7 Seed culture Simple culture technique Scale-up production Shrimp aquaculture water |
| url | http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2022.e47 |
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