Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats
Peptic ulcer disease is an injury of the alimentary tract that leads to a mucosal defect reaching the submucosa. This study aimed to formulate and optimize omega-3 oil as a self-nanoemulsifying drug delivery system (SNEDDS) to achieve oil dispersion in the nano-range in the stomach to augment omega-...
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MDPI AG
2020-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/2/140 |
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| author | Osama A. A. Ahmed Usama A. Fahmy Rana Bakhaidar Mohamed A. El-Moselhy Solomon Z. Okbazghi Al-Shaimaa F. Ahmed Asmaa S. A. Hammad Nabil A. Alhakamy |
| author_facet | Osama A. A. Ahmed Usama A. Fahmy Rana Bakhaidar Mohamed A. El-Moselhy Solomon Z. Okbazghi Al-Shaimaa F. Ahmed Asmaa S. A. Hammad Nabil A. Alhakamy |
| author_sort | Osama A. A. Ahmed |
| collection | DOAJ |
| description | Peptic ulcer disease is an injury of the alimentary tract that leads to a mucosal defect reaching the submucosa. This study aimed to formulate and optimize omega-3 oil as a self-nanoemulsifying drug delivery system (SNEDDS) to achieve oil dispersion in the nano-range in the stomach to augment omega-3 oil gastric ulcer protection efficacy. Three SNEDDS components were selected as the design factors: the concentrations of the oil omega-3 (X1, 10−30%), the surfactant tween 20 and Kolliphor mixture (X2, 20−40%), and the cosurfactant transcutol (X3, 40−60%). The mixture experimental design proposed twenty-three formulations with varying omega-3 SNEDDS formulation component percentages. The optimized omega-3 SNEDDS formula was investigated for gastric ulcer protective effects by evaluating the ulcer index and by the determination of gastric mucosa oxidative stress parameters. Results revealed that optimized omega-3-SNEDDS achieved significant improvement in the gastric ulcer index in comparison with pure omega-3 oil. Histopathological findings confirmed the protective effect of the formulated optimized omega-3 SNEDDS in comparison with omega-3 oil. These findings suggest that formulation of omega-3 in the form of a SNEDDS would be more effective in gastric ulcer protection than the administration of omega-3 as a crude oil. |
| first_indexed | 2024-04-13T07:16:05Z |
| format | Article |
| id | doaj.art-cd4d47b708bc4bee802a549119d07ae5 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-13T07:16:05Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-cd4d47b708bc4bee802a549119d07ae52022-12-22T02:56:45ZengMDPI AGPharmaceutics1999-49232020-02-0112214010.3390/pharmaceutics12020140pharmaceutics12020140Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in RatsOsama A. A. Ahmed0Usama A. Fahmy1Rana Bakhaidar2Mohamed A. El-Moselhy3Solomon Z. Okbazghi4Al-Shaimaa F. Ahmed5Asmaa S. A. Hammad6Nabil A. Alhakamy7Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmacology, School of Pharmacy, Ibn Sina national college, Jeddah 22413, Saudi ArabiaGlobal Analytical and Pharmaceutical Development, Alexion Pharmaceuticals, New Haven, CT 06510, USADepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaPeptic ulcer disease is an injury of the alimentary tract that leads to a mucosal defect reaching the submucosa. This study aimed to formulate and optimize omega-3 oil as a self-nanoemulsifying drug delivery system (SNEDDS) to achieve oil dispersion in the nano-range in the stomach to augment omega-3 oil gastric ulcer protection efficacy. Three SNEDDS components were selected as the design factors: the concentrations of the oil omega-3 (X1, 10−30%), the surfactant tween 20 and Kolliphor mixture (X2, 20−40%), and the cosurfactant transcutol (X3, 40−60%). The mixture experimental design proposed twenty-three formulations with varying omega-3 SNEDDS formulation component percentages. The optimized omega-3 SNEDDS formula was investigated for gastric ulcer protective effects by evaluating the ulcer index and by the determination of gastric mucosa oxidative stress parameters. Results revealed that optimized omega-3-SNEDDS achieved significant improvement in the gastric ulcer index in comparison with pure omega-3 oil. Histopathological findings confirmed the protective effect of the formulated optimized omega-3 SNEDDS in comparison with omega-3 oil. These findings suggest that formulation of omega-3 in the form of a SNEDDS would be more effective in gastric ulcer protection than the administration of omega-3 as a crude oil.https://www.mdpi.com/1999-4923/12/2/140natural productfish oilnanoemulsionfactorial designgastric ulcer |
| spellingShingle | Osama A. A. Ahmed Usama A. Fahmy Rana Bakhaidar Mohamed A. El-Moselhy Solomon Z. Okbazghi Al-Shaimaa F. Ahmed Asmaa S. A. Hammad Nabil A. Alhakamy Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats Pharmaceutics natural product fish oil nanoemulsion factorial design gastric ulcer |
| title | Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats |
| title_full | Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats |
| title_fullStr | Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats |
| title_full_unstemmed | Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats |
| title_short | Omega-3 Self-Nanoemulsion Role in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats |
| title_sort | omega 3 self nanoemulsion role in gastroprotection against indomethacin induced gastric injury in rats |
| topic | natural product fish oil nanoemulsion factorial design gastric ulcer |
| url | https://www.mdpi.com/1999-4923/12/2/140 |
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