Free Radical Scavenging and Antioxidant Activities of Silymarin Components
Silymarin is an over the counter food supplement that is sold as a liver enhancement and liver protection preparation. It is a major constituent of the seeds of Silybum marianum which is composed of a mixture of seven major components and several minor compounds. The seven major components: taxifoli...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-12-01
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| Series: | Antioxidants |
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| Online Access: | http://www.mdpi.com/2076-3921/2/4/398 |
| _version_ | 1797726531608379392 |
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| author | Kevin P. Anthony Mahmoud A. Saleh |
| author_facet | Kevin P. Anthony Mahmoud A. Saleh |
| author_sort | Kevin P. Anthony |
| collection | DOAJ |
| description | Silymarin is an over the counter food supplement that is sold as a liver enhancement and liver protection preparation. It is a major constituent of the seeds of Silybum marianum which is composed of a mixture of seven major components and several minor compounds. The seven major components: taxifolin, silychristin, silydianin, silybin A, silybin B, iso-silybin A and iso-silybin B were isolated and purified from the crude mixture of silymarin using preparative high performance liquid chromatography to determine which were the most effective for liver protection. Free radical scavenging, hydroxyl radical antioxidant capacity, oxygen radical antioxidant capacity, trolox-equivalent antioxidant capacity and total antioxidant capacity antioxidant activities were determined for each of the individual purified components as well as the crude silymarin mixture. Taxifolin was the most effective component for scavenging free radicals in the DPPH assay with an EC50 of 32 µM far more effective than all other components which showed EC50 ranging from 115 to 855 µM. Taxifolin was also found to be the most effective antioxidant in the oxygen radical antioxidant capacity (ORAC) assay with a trolox equivalent of 2.43 and the second most effective in the hydroxyl radical antioxidant capacity (HORAC) assay with a gallic acid equivalent of 0.57. Other antioxidants assays did not show significant differences between samples. |
| first_indexed | 2024-03-12T10:46:55Z |
| format | Article |
| id | doaj.art-cd4f9f049eb245a781ee0e1b7e434294 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T10:46:55Z |
| publishDate | 2013-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-cd4f9f049eb245a781ee0e1b7e4342942023-09-02T07:28:36ZengMDPI AGAntioxidants2076-39212013-12-012439840710.3390/antiox2040398antiox2040398Free Radical Scavenging and Antioxidant Activities of Silymarin ComponentsKevin P. Anthony0Mahmoud A. Saleh1Department of Chemistry, Texas Southern University, Houston, TX 77004, USADepartment of Chemistry, Texas Southern University, Houston, TX 77004, USASilymarin is an over the counter food supplement that is sold as a liver enhancement and liver protection preparation. It is a major constituent of the seeds of Silybum marianum which is composed of a mixture of seven major components and several minor compounds. The seven major components: taxifolin, silychristin, silydianin, silybin A, silybin B, iso-silybin A and iso-silybin B were isolated and purified from the crude mixture of silymarin using preparative high performance liquid chromatography to determine which were the most effective for liver protection. Free radical scavenging, hydroxyl radical antioxidant capacity, oxygen radical antioxidant capacity, trolox-equivalent antioxidant capacity and total antioxidant capacity antioxidant activities were determined for each of the individual purified components as well as the crude silymarin mixture. Taxifolin was the most effective component for scavenging free radicals in the DPPH assay with an EC50 of 32 µM far more effective than all other components which showed EC50 ranging from 115 to 855 µM. Taxifolin was also found to be the most effective antioxidant in the oxygen radical antioxidant capacity (ORAC) assay with a trolox equivalent of 2.43 and the second most effective in the hydroxyl radical antioxidant capacity (HORAC) assay with a gallic acid equivalent of 0.57. Other antioxidants assays did not show significant differences between samples.http://www.mdpi.com/2076-3921/2/4/398Silybum marianummilk thistlefood supplementshepatoprotection |
| spellingShingle | Kevin P. Anthony Mahmoud A. Saleh Free Radical Scavenging and Antioxidant Activities of Silymarin Components Antioxidants Silybum marianum milk thistle food supplements hepatoprotection |
| title | Free Radical Scavenging and Antioxidant Activities of Silymarin Components |
| title_full | Free Radical Scavenging and Antioxidant Activities of Silymarin Components |
| title_fullStr | Free Radical Scavenging and Antioxidant Activities of Silymarin Components |
| title_full_unstemmed | Free Radical Scavenging and Antioxidant Activities of Silymarin Components |
| title_short | Free Radical Scavenging and Antioxidant Activities of Silymarin Components |
| title_sort | free radical scavenging and antioxidant activities of silymarin components |
| topic | Silybum marianum milk thistle food supplements hepatoprotection |
| url | http://www.mdpi.com/2076-3921/2/4/398 |
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