T Cell Specific BOB.1/OBF.1 Expression Promotes Germinal Center Response and T Helper Cell Differentiation
The transcriptional co-activator BOB.1/OBF.1 is expressed in both B and T cells. The main characteristic of conventional BOB.1/OBF.1 deficient mice is the complete absence of germinal centers (GCs). This defect was mainly attributed to the defective B cell compartment. However, it is unknown whether...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.889564/full |
| _version_ | 1811234771503677440 |
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| author | Annika C. Betzler Jasmin Ezić Tsima Abou Kors Thomas K. Hoffmann Thomas Wirth Cornelia Brunner |
| author_facet | Annika C. Betzler Jasmin Ezić Tsima Abou Kors Thomas K. Hoffmann Thomas Wirth Cornelia Brunner |
| author_sort | Annika C. Betzler |
| collection | DOAJ |
| description | The transcriptional co-activator BOB.1/OBF.1 is expressed in both B and T cells. The main characteristic of conventional BOB.1/OBF.1 deficient mice is the complete absence of germinal centers (GCs). This defect was mainly attributed to the defective B cell compartment. However, it is unknown whether and how BOB.1/OBF.1 expression in T cells contributes to the GC reaction. To finally clarify this question, we studied the in vivo function of BOB.1/OBF.1 in CD4+ T and follicular T helper (TFH) cell subpopulations by conditional mutagenesis, in the presence of immunocompetent B lymphocytes. BOB.1/OBF.1 deletion in CD4+ T as well as TFH cells resulted in impaired GC formation demonstrating that the impaired GC reaction described for conventional BOB.1/OBF.1-deficient mice cannot exclusively be traced back to the B cell compartment. Furthermore, we show a requirement of BOB.1/OBF.1 for T helper (TH) cell subsets, particularly for TFH cell differentiation. |
| first_indexed | 2024-04-12T11:41:14Z |
| format | Article |
| id | doaj.art-cd50db055ba54440964af69597aaba3b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T11:41:14Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-cd50db055ba54440964af69597aaba3b2022-12-22T03:34:37ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-05-011310.3389/fimmu.2022.889564889564T Cell Specific BOB.1/OBF.1 Expression Promotes Germinal Center Response and T Helper Cell DifferentiationAnnika C. Betzler0Jasmin Ezić1Tsima Abou Kors2Thomas K. Hoffmann3Thomas Wirth4Cornelia Brunner5Department of Oto-Rhino-Laryngology, Ulm University Medical Center, Ulm, GermanyDepartment of Oto-Rhino-Laryngology, Ulm University Medical Center, Ulm, GermanyDepartment of Oto-Rhino-Laryngology, Ulm University Medical Center, Ulm, GermanyDepartment of Oto-Rhino-Laryngology, Ulm University Medical Center, Ulm, GermanyDepartment of Physiological Chemistry, Ulm University, Ulm, GermanyDepartment of Oto-Rhino-Laryngology, Ulm University Medical Center, Ulm, GermanyThe transcriptional co-activator BOB.1/OBF.1 is expressed in both B and T cells. The main characteristic of conventional BOB.1/OBF.1 deficient mice is the complete absence of germinal centers (GCs). This defect was mainly attributed to the defective B cell compartment. However, it is unknown whether and how BOB.1/OBF.1 expression in T cells contributes to the GC reaction. To finally clarify this question, we studied the in vivo function of BOB.1/OBF.1 in CD4+ T and follicular T helper (TFH) cell subpopulations by conditional mutagenesis, in the presence of immunocompetent B lymphocytes. BOB.1/OBF.1 deletion in CD4+ T as well as TFH cells resulted in impaired GC formation demonstrating that the impaired GC reaction described for conventional BOB.1/OBF.1-deficient mice cannot exclusively be traced back to the B cell compartment. Furthermore, we show a requirement of BOB.1/OBF.1 for T helper (TH) cell subsets, particularly for TFH cell differentiation.https://www.frontiersin.org/articles/10.3389/fimmu.2022.889564/fullBOB.1/OBF.1germinal centerT cell differentiationfollicular T helper cellshumoral immunitytranscriptional co-activator |
| spellingShingle | Annika C. Betzler Jasmin Ezić Tsima Abou Kors Thomas K. Hoffmann Thomas Wirth Cornelia Brunner T Cell Specific BOB.1/OBF.1 Expression Promotes Germinal Center Response and T Helper Cell Differentiation Frontiers in Immunology BOB.1/OBF.1 germinal center T cell differentiation follicular T helper cells humoral immunity transcriptional co-activator |
| title | T Cell Specific BOB.1/OBF.1 Expression Promotes Germinal Center Response and T Helper Cell Differentiation |
| title_full | T Cell Specific BOB.1/OBF.1 Expression Promotes Germinal Center Response and T Helper Cell Differentiation |
| title_fullStr | T Cell Specific BOB.1/OBF.1 Expression Promotes Germinal Center Response and T Helper Cell Differentiation |
| title_full_unstemmed | T Cell Specific BOB.1/OBF.1 Expression Promotes Germinal Center Response and T Helper Cell Differentiation |
| title_short | T Cell Specific BOB.1/OBF.1 Expression Promotes Germinal Center Response and T Helper Cell Differentiation |
| title_sort | t cell specific bob 1 obf 1 expression promotes germinal center response and t helper cell differentiation |
| topic | BOB.1/OBF.1 germinal center T cell differentiation follicular T helper cells humoral immunity transcriptional co-activator |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.889564/full |
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