RETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1)
RETRACTED Herpes simplex viruses types 1 (HSV-1) and 2 (HSV-2) are among the most common viruses in the human population. The clinical manifestations of HSV infection vary widely, which necessitates reliable molecular methods for the timely diagnosis of herpes virus infection, as well as for detecti...
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| Format: | Article |
| Language: | English |
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Central Research Institute for Epidemiology
2020-07-01
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| Series: | Вопросы вирусологии |
| Subjects: | |
| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/385/259 |
| _version_ | 1797782305845018624 |
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| author | O. V. Sergeyev R. E. Bosh’ian I. F. Barinsky |
| author_facet | O. V. Sergeyev R. E. Bosh’ian I. F. Barinsky |
| author_sort | O. V. Sergeyev |
| collection | DOAJ |
| description | RETRACTED
Herpes simplex viruses types 1 (HSV-1) and 2 (HSV-2) are among the most common viruses in the human population. The clinical manifestations of HSV infection vary widely, which necessitates reliable molecular methods for the timely diagnosis of herpes virus infection, as well as for detection of mutations in the genes responsible for drug resistance. PCR is often unable to detect HSV isolates with nucleotide substitutions at the primer binding site. Sanger sequencing of the whole genome reveals mutations mainly at the consensus level, which accumulate at advanced stages of viral infection. High-throughput sequencing (HTS, next generation sequencing) offers an obvious advantage both in early diagnosis of herpes virus infection and identification of HSV variants. |
| first_indexed | 2024-03-13T00:09:06Z |
| format | Article |
| id | doaj.art-cd51045fc5364b4f9533af108d602b3a |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:09:06Z |
| publishDate | 2020-07-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-cd51045fc5364b4f9533af108d602b3a2023-07-12T19:57:43ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972020-07-0165312613110.36233/0507-4088-2020-65-3-126-131335RETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1)O. V. Sergeyev0https://orcid.org/0000-0003-3407-2224R. E. Bosh’ian1https://orcid.org/0000-0003-4789-4964I. F. Barinsky2https://orcid.org/0000-0002-9909-3598I.M. Sechenov First Moscow State Medical University (Sechenov University)I.M. Sechenov First Moscow State Medical University (Sechenov University)National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. GamaleyaRETRACTED Herpes simplex viruses types 1 (HSV-1) and 2 (HSV-2) are among the most common viruses in the human population. The clinical manifestations of HSV infection vary widely, which necessitates reliable molecular methods for the timely diagnosis of herpes virus infection, as well as for detection of mutations in the genes responsible for drug resistance. PCR is often unable to detect HSV isolates with nucleotide substitutions at the primer binding site. Sanger sequencing of the whole genome reveals mutations mainly at the consensus level, which accumulate at advanced stages of viral infection. High-throughput sequencing (HTS, next generation sequencing) offers an obvious advantage both in early diagnosis of herpes virus infection and identification of HSV variants.https://virusjour.crie.ru/jour/article/viewFile/385/259herpes simplex virusmolecular diagnosticsgenomevariabilityhighthroughput sequencinghtsnext generation sequencingwhole genome sequencingpcrmetagenomics |
| spellingShingle | O. V. Sergeyev R. E. Bosh’ian I. F. Barinsky RETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1) Вопросы вирусологии herpes simplex virus molecular diagnostics genome variability highthroughput sequencing hts next generation sequencing whole genome sequencing pcr metagenomics |
| title | RETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1) |
| title_full | RETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1) |
| title_fullStr | RETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1) |
| title_full_unstemmed | RETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1) |
| title_short | RETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1) |
| title_sort | retracted high throughput sequencing in diagnostics and prevention of herpes simplex virus infection herpesviridae alphaherpesvirinae simplexvirus human alphaherpesvirus 1 |
| topic | herpes simplex virus molecular diagnostics genome variability highthroughput sequencing hts next generation sequencing whole genome sequencing pcr metagenomics |
| url | https://virusjour.crie.ru/jour/article/viewFile/385/259 |
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