Combining a Mutant Allele of <i>FAD2-1A</i> with <i>HD</i> Improves the ω-6/ω-3 Ratio in Soybeans
The intake of foods with unbalanced ω-6/ω-3 ratios causes various health problems. Commodity soybeans generally have a ω-6/ω-3 ratio of 6–7:1. The recommended ratio in terms of health benefits is <4:1. This study aimed to identify the appropriate combination of mutant alleles that can reduce the...
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MDPI AG
2023-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/3/913 |
| _version_ | 1827751849833267200 |
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| author | Hwayeop Kim Hyun Jo Jeong-Dong Lee |
| author_facet | Hwayeop Kim Hyun Jo Jeong-Dong Lee |
| author_sort | Hwayeop Kim |
| collection | DOAJ |
| description | The intake of foods with unbalanced ω-6/ω-3 ratios causes various health problems. Commodity soybeans generally have a ω-6/ω-3 ratio of 6–7:1. The recommended ratio in terms of health benefits is <4:1. This study aimed to identify the appropriate combination of mutant alleles that can reduce the ω-6/ω-3 ratio using three segregating soybean populations. F<sub>2</sub> individuals from each population were genotyped for three different alleles of microsomal delta-12 fatty acid desaturase 2 enzyme (<i>FAD2-1A</i>) and an allele of homeodomain-like transcriptional regulator (<i>HD</i>) genes, and their five major fatty acids were assessed. F<sub>2</sub> seeds carrying both <i>fad2-1a</i> and <i>hd</i> had slightly different ω-6/ω-3 ratios according to the different <i>fad2-1a</i> alleles. The <i>fad2-1a<sub>DEL</sub></i>, <i>fad2-1a<sub>S117N</sub></i>, and <i>fad2-1a<sub>W293STOP</sub></i> alleles combined with a <i>hd</i> allele resulted in ω-6/ω-3 ratios with a range of 1.9–2.7:1, 2.7–3.9:1, and 2.6–3.6:1 in soybean seeds, respectively. This study revealed that the induction of mutations in <i>FAD2-1A<sub>DEL</sub></i> and <i>HD</i> was the most efficient strategy to improve the ω-6/ω-3 ratio and elevate the ω-3 fatty acid concentrations in soybean seeds. These results provide useful information in soybean breeding programs to release a new soybean cultivar with a lower ω-6/ω-3 ratio and elevated ω-3 fatty acids, which can be a beneficial ingredient for soybean-based foods. |
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| language | English |
| last_indexed | 2024-03-11T07:02:54Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-cd52300a285f488ba2472c81877df5fd2023-11-17T09:08:06ZengMDPI AGAgronomy2073-43952023-03-0113391310.3390/agronomy13030913Combining a Mutant Allele of <i>FAD2-1A</i> with <i>HD</i> Improves the ω-6/ω-3 Ratio in SoybeansHwayeop Kim0Hyun Jo1Jeong-Dong Lee2Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaThe intake of foods with unbalanced ω-6/ω-3 ratios causes various health problems. Commodity soybeans generally have a ω-6/ω-3 ratio of 6–7:1. The recommended ratio in terms of health benefits is <4:1. This study aimed to identify the appropriate combination of mutant alleles that can reduce the ω-6/ω-3 ratio using three segregating soybean populations. F<sub>2</sub> individuals from each population were genotyped for three different alleles of microsomal delta-12 fatty acid desaturase 2 enzyme (<i>FAD2-1A</i>) and an allele of homeodomain-like transcriptional regulator (<i>HD</i>) genes, and their five major fatty acids were assessed. F<sub>2</sub> seeds carrying both <i>fad2-1a</i> and <i>hd</i> had slightly different ω-6/ω-3 ratios according to the different <i>fad2-1a</i> alleles. The <i>fad2-1a<sub>DEL</sub></i>, <i>fad2-1a<sub>S117N</sub></i>, and <i>fad2-1a<sub>W293STOP</sub></i> alleles combined with a <i>hd</i> allele resulted in ω-6/ω-3 ratios with a range of 1.9–2.7:1, 2.7–3.9:1, and 2.6–3.6:1 in soybean seeds, respectively. This study revealed that the induction of mutations in <i>FAD2-1A<sub>DEL</sub></i> and <i>HD</i> was the most efficient strategy to improve the ω-6/ω-3 ratio and elevate the ω-3 fatty acid concentrations in soybean seeds. These results provide useful information in soybean breeding programs to release a new soybean cultivar with a lower ω-6/ω-3 ratio and elevated ω-3 fatty acids, which can be a beneficial ingredient for soybean-based foods.https://www.mdpi.com/2073-4395/13/3/913α-linoleic acid<i>FAD2-1</i>homeodomain transcription factorsoybean foodω-6/ω-3 ratio |
| spellingShingle | Hwayeop Kim Hyun Jo Jeong-Dong Lee Combining a Mutant Allele of <i>FAD2-1A</i> with <i>HD</i> Improves the ω-6/ω-3 Ratio in Soybeans Agronomy α-linoleic acid <i>FAD2-1</i> homeodomain transcription factor soybean food ω-6/ω-3 ratio |
| title | Combining a Mutant Allele of <i>FAD2-1A</i> with <i>HD</i> Improves the ω-6/ω-3 Ratio in Soybeans |
| title_full | Combining a Mutant Allele of <i>FAD2-1A</i> with <i>HD</i> Improves the ω-6/ω-3 Ratio in Soybeans |
| title_fullStr | Combining a Mutant Allele of <i>FAD2-1A</i> with <i>HD</i> Improves the ω-6/ω-3 Ratio in Soybeans |
| title_full_unstemmed | Combining a Mutant Allele of <i>FAD2-1A</i> with <i>HD</i> Improves the ω-6/ω-3 Ratio in Soybeans |
| title_short | Combining a Mutant Allele of <i>FAD2-1A</i> with <i>HD</i> Improves the ω-6/ω-3 Ratio in Soybeans |
| title_sort | combining a mutant allele of i fad2 1a i with i hd i improves the ω 6 ω 3 ratio in soybeans |
| topic | α-linoleic acid <i>FAD2-1</i> homeodomain transcription factor soybean food ω-6/ω-3 ratio |
| url | https://www.mdpi.com/2073-4395/13/3/913 |
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