Production of lipase by the deep-sea bacterium Idiomarina zobellii; Identification of effective factors and optimization of production process
Introduction: In this study, the deep-sea bacterium, Idiomarina zobellii, was used as a new source for the production of lipase. Lipases from halophilic bacteria are attractive for use in diverse industrial and biotechnological applications. Materials and methods: The optimal temperature and pH for...
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| Format: | Article |
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University of Isfahan
2018-12-01
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| Series: | Biological Journal of Microorganism |
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| Online Access: | http://bjm.ui.ac.ir/article_22830_5d580887ee498b307e1bc476d27b7a25.pdf |
| _version_ | 1818026807636525056 |
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| author | Shahrbanoo Sadeghi-Dastjerdi Mehdi Ebrahimi Rozbeh Yalfani |
| author_facet | Shahrbanoo Sadeghi-Dastjerdi Mehdi Ebrahimi Rozbeh Yalfani |
| author_sort | Shahrbanoo Sadeghi-Dastjerdi |
| collection | DOAJ |
| description | Introduction: In this study, the deep-sea bacterium, Idiomarina zobellii, was used as a new source for the production of lipase. Lipases from halophilic bacteria are attractive for use in diverse industrial and biotechnological applications. Materials and methods: The optimal temperature and pH for the activity of I. zobellii lipase was determined using different temperature and pH ranging from 30-60 °C and 7-11, respectively. The growth conditions influencing lipase production were determined using two-level fractional factorial Plackett–Burman design. The optimum levels of effective factors for production process were determined by using the response surface central composite design. Results: The optimum temperature and pH of I. zobellii lipase in the crude mixture were 7-8 and 50 °C, respectively. Among the screened 10 factors, MgCl2, yeast extract, sodium bicarbonate, ammonium nitrate concentrations and temperature were found to be effectively important. The highest productivity level was achieved at 42°C temperature and MgCl2 7.18 (g/L), yeast extract 1.1 (g/L), NH4NO3 3.65 (g/L) and NaHCO3 0.11 (g/L) concentrations. When the predicted optimum levels of effective factors were employed, the enzyme activity almost doubled (0.8 comparing to 0.39 U/ml) of the initial. Discussion and conclusion: The Idiomarina zobellii originated lipase is an alkalophile enzyme. A significant increase in enzyme production, as large as 2.09 folds, is achievable under optimized conditions that favours biotechnology and industrial applications. |
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| id | doaj.art-cd54ee0bdf6d4df3af8bc2ac987a3c41 |
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| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-12-10T04:37:52Z |
| publishDate | 2018-12-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-cd54ee0bdf6d4df3af8bc2ac987a3c412022-12-22T02:01:58ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812018-12-01728415210.22108/bjm.2018.108098.109622830Production of lipase by the deep-sea bacterium Idiomarina zobellii; Identification of effective factors and optimization of production processShahrbanoo Sadeghi-Dastjerdi0Mehdi Ebrahimi1Rozbeh Yalfani2M.Sc student, Department of Biochemistry and Biophysics, Faculty of Biological sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, IranAssistant Professor, Department of Biochemistry and Biophysics, Faculty of Biological sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, IranAssistant Professor, Department of Biochemistry and Biophysics, Faculty of Biological sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, IranIntroduction: In this study, the deep-sea bacterium, Idiomarina zobellii, was used as a new source for the production of lipase. Lipases from halophilic bacteria are attractive for use in diverse industrial and biotechnological applications. Materials and methods: The optimal temperature and pH for the activity of I. zobellii lipase was determined using different temperature and pH ranging from 30-60 °C and 7-11, respectively. The growth conditions influencing lipase production were determined using two-level fractional factorial Plackett–Burman design. The optimum levels of effective factors for production process were determined by using the response surface central composite design. Results: The optimum temperature and pH of I. zobellii lipase in the crude mixture were 7-8 and 50 °C, respectively. Among the screened 10 factors, MgCl2, yeast extract, sodium bicarbonate, ammonium nitrate concentrations and temperature were found to be effectively important. The highest productivity level was achieved at 42°C temperature and MgCl2 7.18 (g/L), yeast extract 1.1 (g/L), NH4NO3 3.65 (g/L) and NaHCO3 0.11 (g/L) concentrations. When the predicted optimum levels of effective factors were employed, the enzyme activity almost doubled (0.8 comparing to 0.39 U/ml) of the initial. Discussion and conclusion: The Idiomarina zobellii originated lipase is an alkalophile enzyme. A significant increase in enzyme production, as large as 2.09 folds, is achievable under optimized conditions that favours biotechnology and industrial applications.http://bjm.ui.ac.ir/article_22830_5d580887ee498b307e1bc476d27b7a25.pdfLipaseIdiomarina zobelliiPlackett–Burman DesignResponse Surface Methodology (RSM)Deep-sea Bacterium |
| spellingShingle | Shahrbanoo Sadeghi-Dastjerdi Mehdi Ebrahimi Rozbeh Yalfani Production of lipase by the deep-sea bacterium Idiomarina zobellii; Identification of effective factors and optimization of production process Biological Journal of Microorganism Lipase Idiomarina zobellii Plackett–Burman Design Response Surface Methodology (RSM) Deep-sea Bacterium |
| title | Production of lipase by the deep-sea bacterium Idiomarina zobellii; Identification of effective factors and optimization of production process |
| title_full | Production of lipase by the deep-sea bacterium Idiomarina zobellii; Identification of effective factors and optimization of production process |
| title_fullStr | Production of lipase by the deep-sea bacterium Idiomarina zobellii; Identification of effective factors and optimization of production process |
| title_full_unstemmed | Production of lipase by the deep-sea bacterium Idiomarina zobellii; Identification of effective factors and optimization of production process |
| title_short | Production of lipase by the deep-sea bacterium Idiomarina zobellii; Identification of effective factors and optimization of production process |
| title_sort | production of lipase by the deep sea bacterium idiomarina zobellii identification of effective factors and optimization of production process |
| topic | Lipase Idiomarina zobellii Plackett–Burman Design Response Surface Methodology (RSM) Deep-sea Bacterium |
| url | http://bjm.ui.ac.ir/article_22830_5d580887ee498b307e1bc476d27b7a25.pdf |
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