Disintegration promotes protospacer integration by the Cas1-Cas2 complex

Disintegration promotes protospacer integration by the Cas1-Cas2 complex

‘Disintegration’—the reversal of transposon DNA integration at a target site—is regarded as an abortive off-pathway reaction. Here, we challenge this view with a biochemical investigation of the mechanism of protospacer insertion, which is mechanistically analogous to DNA transposition, by the Strep...

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Bibliographic Details
Main Authors: Chien-Hui Ma, Kamyab Javanmardi, Ilya J Finkelstein, Makkuni Jayaram
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-08-01
Series:eLife
Subjects:
CRISPR
adaptation
transposition
Online Access:https://elifesciences.org/articles/65763

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