Genetic Polymorphisms of CCL22 and CCR4 in Patients with Lung Cancer
Background: An association between lung cancer and chemokines has been advocated in the recent years. This study aims at investigating the association between lung cancer and 16C/A single nucleotide polymorphism (SNP) (rs. 4359426) in C-C motif chemokine 22 (CCL22) as well as C1014T SNP (rs. 2228428...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2015-07-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/index.php/IJMS/article/view/951 |
| _version_ | 1818538424115658752 |
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| author | Nasrollah Erfani Ahmadi-Sina Nedaei Ahmadi Mohammad Ali Ghayumi Zahra Mojtahedi |
| author_facet | Nasrollah Erfani Ahmadi-Sina Nedaei Ahmadi Mohammad Ali Ghayumi Zahra Mojtahedi |
| author_sort | Nasrollah Erfani |
| collection | DOAJ |
| description | Background: An association between lung cancer and chemokines has been advocated in the recent years. This study aims at investigating the association between lung cancer and 16C/A single nucleotide polymorphism (SNP) (rs. 4359426) in C-C motif chemokine 22 (CCL22) as well as C1014T SNP (rs. 2228428) in C-C chemokine receptor type 4 (CCR4), which serves as the receptor for CCL22.
Methods: Genotyping was performed in 148 lung cancer patients and 148 normal controls using Polymerase Chain Reaction-Restriction-Fragment Length Polymorphism (PCR-RFLP). The data were verified by direct automated sequencing.
Results: Frequencies of CC, CA and AA genotypes of 16C/A SNP in CCL22 gene were 112 (75.7%), 33 (22.3%) and 3 (2.0%) in patients, and 119 (80.4%), 24 (16.2%) and 5 (3.4%) in controls respectively. No significant differences were observed in genotype frequencies at this position between cases and controls (P=0.34). Moreover, there was no significant association between CCL22 polymorphism and types of lung cancer in patients. The distribution of CC, CT and TT genotypes of C1014T SNP in CCR4 gene, was 76 (51.4%), 60 (40.5%) and 12 (8.1%) in patients, and 80 (54.1%), 49 (33.1%) and 19 (12.8%) in controls respectively. No statistically significant differences were observed in genotypes frequencies of CCR4 gene between patients and controls (P=0.24). The genotype inherited by patients observed not to be associated with the type of lung cancer (P>0.05).
Conclusion: Results reveal that CCL22 gene polymorphism at position 16C/A and CCR4 gene polymorphism at position C1014T, appear not to be associated with susceptibility to lung cancer. |
| first_indexed | 2024-12-11T21:28:48Z |
| format | Article |
| id | doaj.art-cd583ede93724bfea397dc05a946ccb8 |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-12-11T21:28:48Z |
| publishDate | 2015-07-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-cd583ede93724bfea397dc05a946ccb82022-12-22T00:50:15ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882015-07-01394367373Genetic Polymorphisms of CCL22 and CCR4 in Patients with Lung CancerNasrollah Erfani0Ahmadi-Sina Nedaei Ahmadi1Mohammad Ali Ghayumi2Zahra Mojtahedi3Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Student Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Department of Internal Medicine, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, IranShiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Background: An association between lung cancer and chemokines has been advocated in the recent years. This study aims at investigating the association between lung cancer and 16C/A single nucleotide polymorphism (SNP) (rs. 4359426) in C-C motif chemokine 22 (CCL22) as well as C1014T SNP (rs. 2228428) in C-C chemokine receptor type 4 (CCR4), which serves as the receptor for CCL22. Methods: Genotyping was performed in 148 lung cancer patients and 148 normal controls using Polymerase Chain Reaction-Restriction-Fragment Length Polymorphism (PCR-RFLP). The data were verified by direct automated sequencing. Results: Frequencies of CC, CA and AA genotypes of 16C/A SNP in CCL22 gene were 112 (75.7%), 33 (22.3%) and 3 (2.0%) in patients, and 119 (80.4%), 24 (16.2%) and 5 (3.4%) in controls respectively. No significant differences were observed in genotype frequencies at this position between cases and controls (P=0.34). Moreover, there was no significant association between CCL22 polymorphism and types of lung cancer in patients. The distribution of CC, CT and TT genotypes of C1014T SNP in CCR4 gene, was 76 (51.4%), 60 (40.5%) and 12 (8.1%) in patients, and 80 (54.1%), 49 (33.1%) and 19 (12.8%) in controls respectively. No statistically significant differences were observed in genotypes frequencies of CCR4 gene between patients and controls (P=0.24). The genotype inherited by patients observed not to be associated with the type of lung cancer (P>0.05). Conclusion: Results reveal that CCL22 gene polymorphism at position 16C/A and CCR4 gene polymorphism at position C1014T, appear not to be associated with susceptibility to lung cancer.http://ijms.sums.ac.ir/index.php/IJMS/article/view/951PolymorphismGeneticLung NeoplasmsChemokine CCL22CCR4 |
| spellingShingle | Nasrollah Erfani Ahmadi-Sina Nedaei Ahmadi Mohammad Ali Ghayumi Zahra Mojtahedi Genetic Polymorphisms of CCL22 and CCR4 in Patients with Lung Cancer Iranian Journal of Medical Sciences Polymorphism Genetic Lung Neoplasms Chemokine CCL22 CCR4 |
| title | Genetic Polymorphisms of CCL22 and CCR4 in Patients with Lung Cancer |
| title_full | Genetic Polymorphisms of CCL22 and CCR4 in Patients with Lung Cancer |
| title_fullStr | Genetic Polymorphisms of CCL22 and CCR4 in Patients with Lung Cancer |
| title_full_unstemmed | Genetic Polymorphisms of CCL22 and CCR4 in Patients with Lung Cancer |
| title_short | Genetic Polymorphisms of CCL22 and CCR4 in Patients with Lung Cancer |
| title_sort | genetic polymorphisms of ccl22 and ccr4 in patients with lung cancer |
| topic | Polymorphism Genetic Lung Neoplasms Chemokine CCL22 CCR4 |
| url | http://ijms.sums.ac.ir/index.php/IJMS/article/view/951 |
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