Gadolinium-Labelled Cell Scaffolds to Follow-up Cell Transplantation by Magnetic Resonance Imaging
Cell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated...
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MDPI AG
2019-07-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/10/3/28 |
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| author | Valeria Catanzaro Giuseppe Digilio Federico Capuana Sergio Padovan Juan C. Cutrin Fabio Carniato Stefano Porta Cristina Grange Nenad Filipović Magdalena Stevanović |
| author_facet | Valeria Catanzaro Giuseppe Digilio Federico Capuana Sergio Padovan Juan C. Cutrin Fabio Carniato Stefano Porta Cristina Grange Nenad Filipović Magdalena Stevanović |
| author_sort | Valeria Catanzaro |
| collection | DOAJ |
| description | Cell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200−600 μm diameter and 50−80 μm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T<sub>1</sub>-weighted (T<sub>1</sub>w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progressive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs. |
| first_indexed | 2024-04-14T06:18:09Z |
| format | Article |
| id | doaj.art-cd5ab130fc7c4722a8681fcbdf85fe33 |
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| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-14T06:18:09Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-cd5ab130fc7c4722a8681fcbdf85fe332022-12-22T02:08:07ZengMDPI AGJournal of Functional Biomaterials2079-49832019-07-011032810.3390/jfb10030028jfb10030028Gadolinium-Labelled Cell Scaffolds to Follow-up Cell Transplantation by Magnetic Resonance ImagingValeria Catanzaro0Giuseppe Digilio1Federico Capuana2Sergio Padovan3Juan C. Cutrin4Fabio Carniato5Stefano Porta6Cristina Grange7Nenad Filipović8Magdalena Stevanović9Department of Science and Technologic Innovation, Università del Piemonte Orientale “Amedeo Avogadro”, Viale T. Michel 11, I-15121 Alessandria, ItalyDepartment of Science and Technologic Innovation, Università del Piemonte Orientale “Amedeo Avogadro”, Viale T. Michel 11, I-15121 Alessandria, ItalyDepartment of Molecular Biotechnology and Health Science & Center for Molecular Imaging, University of Turin, Via Nizza 52, 10126 Torino, ItalyInstitute for Biostructures and Bioimages (CNR) c/o Molecular Biotechnology Center Via Nizza 52, 10126 Torino, ItalyDepartment of Molecular Biotechnology and Health Science & Center for Molecular Imaging, University of Turin, Via Nizza 52, 10126 Torino, ItalyDepartment of Science and Technologic Innovation, Università del Piemonte Orientale “Amedeo Avogadro”, Viale T. Michel 11, I-15121 Alessandria, ItalyDepartment of Molecular Biotechnology and Health Science & Center for Molecular Imaging, University of Turin, Via Nizza 52, 10126 Torino, ItalyDepartment of Medical Sciences, University of Turin, Via Nizza 52, 10126 Torino, ItalyInstitute of Technical Sciences of the Serbian Academy of Sciences and Arts, Knez Mihailova 35/IV, 11000 Belgrade, SerbiaInstitute of Technical Sciences of the Serbian Academy of Sciences and Arts, Knez Mihailova 35/IV, 11000 Belgrade, SerbiaCell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200−600 μm diameter and 50−80 μm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T<sub>1</sub>-weighted (T<sub>1</sub>w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progressive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs.https://www.mdpi.com/2079-4983/10/3/28cell scaffoldgraft transplantationgadoliniumMagnetic Resonance Imagingbiomaterialhuman mesenchymal stromal cells (hMSC)immune response |
| spellingShingle | Valeria Catanzaro Giuseppe Digilio Federico Capuana Sergio Padovan Juan C. Cutrin Fabio Carniato Stefano Porta Cristina Grange Nenad Filipović Magdalena Stevanović Gadolinium-Labelled Cell Scaffolds to Follow-up Cell Transplantation by Magnetic Resonance Imaging Journal of Functional Biomaterials cell scaffold graft transplantation gadolinium Magnetic Resonance Imaging biomaterial human mesenchymal stromal cells (hMSC) immune response |
| title | Gadolinium-Labelled Cell Scaffolds to Follow-up Cell Transplantation by Magnetic Resonance Imaging |
| title_full | Gadolinium-Labelled Cell Scaffolds to Follow-up Cell Transplantation by Magnetic Resonance Imaging |
| title_fullStr | Gadolinium-Labelled Cell Scaffolds to Follow-up Cell Transplantation by Magnetic Resonance Imaging |
| title_full_unstemmed | Gadolinium-Labelled Cell Scaffolds to Follow-up Cell Transplantation by Magnetic Resonance Imaging |
| title_short | Gadolinium-Labelled Cell Scaffolds to Follow-up Cell Transplantation by Magnetic Resonance Imaging |
| title_sort | gadolinium labelled cell scaffolds to follow up cell transplantation by magnetic resonance imaging |
| topic | cell scaffold graft transplantation gadolinium Magnetic Resonance Imaging biomaterial human mesenchymal stromal cells (hMSC) immune response |
| url | https://www.mdpi.com/2079-4983/10/3/28 |
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