The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting Exopolysaccharides
Endophytes have the potential to enhance the ability of plants to resist salt stress, improving crop development and yield. Therefore, in this study, we isolated an endophyte that produced large amounts of exopolysaccharides (EPSs) from the roots of sea rice and examined its effects on the physiolog...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.03112/full |
| _version_ | 1811259683454844928 |
|---|---|
| author | Liang Sun Liang Sun Liang Sun Peng Lei Peng Lei Peng Lei Qian Wang Qian Wang Junjie Ma Junjie Ma Yijing Zhan Yijing Zhan Yijing Zhan Kang Jiang Kang Jiang Kang Jiang Zongqi Xu Zongqi Xu Zongqi Xu Hong Xu Hong Xu Hong Xu |
| author_facet | Liang Sun Liang Sun Liang Sun Peng Lei Peng Lei Peng Lei Qian Wang Qian Wang Junjie Ma Junjie Ma Yijing Zhan Yijing Zhan Yijing Zhan Kang Jiang Kang Jiang Kang Jiang Zongqi Xu Zongqi Xu Zongqi Xu Hong Xu Hong Xu Hong Xu |
| author_sort | Liang Sun |
| collection | DOAJ |
| description | Endophytes have the potential to enhance the ability of plants to resist salt stress, improving crop development and yield. Therefore, in this study, we isolated an endophyte that produced large amounts of exopolysaccharides (EPSs) from the roots of sea rice and examined its effects on the physiological responses of rice (Oryza sativa L. ssp. japonica “Nipponbare”) seedlings to salt stress using hydroponic experiments. The endophyte was named Pantoea alhagi NX-11 based on its morphological characteristics and 16S ribosomal DNA (rDNA) sequence alignment. Rice seedlings that had been inoculated with P. alhagi NX-11 exhibited a 30.3% increase in fresh weight, a 28.6% increase in root length, a 51.6% increase in shoot length, and a 26.3% increase in chlorophyll content compared with control seedlings under normal conditions. In addition, inoculated rice seedlings had a 37.5% lower malondialdehyde content, a 133% higher K+/Na+ ratio, and a 52.8% higher proline content after 7 days under salt stress, as well as up-regulated expression of proline synthase, down-regulated expression of proline dehydrogenase, and enhanced antioxidant enzyme activities. Interestingly, rice seedlings that were inoculated with an EPS-deficient strain named NX-11eps– that was obtained by atmospheric and room temperature plasma (ARTP) mutagenesis were damaged by salt stress and had similar physiological and biochemical indicators to the control group. Therefore, we speculate that the ability of P. alhagi NX-11 to enhance the salt tolerance of rice seedlings is related to the EPSs it produces. |
| first_indexed | 2024-04-12T18:35:14Z |
| format | Article |
| id | doaj.art-cd5c73ab04cb48d8ad5e39c8c2701425 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T18:35:14Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cd5c73ab04cb48d8ad5e39c8c27014252022-12-22T03:20:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-01-011010.3389/fmicb.2019.03112486750The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting ExopolysaccharidesLiang Sun0Liang Sun1Liang Sun2Peng Lei3Peng Lei4Peng Lei5Qian Wang6Qian Wang7Junjie Ma8Junjie Ma9Yijing Zhan10Yijing Zhan11Yijing Zhan12Kang Jiang13Kang Jiang14Kang Jiang15Zongqi Xu16Zongqi Xu17Zongqi Xu18Hong Xu19Hong Xu20Hong Xu21State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, ChinaCollege of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaJiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, ChinaCollege of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaNanjing Institute for Comprehensive Utilization of Wild Plants, China Co-op, Nanjing, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, ChinaCollege of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, ChinaCollege of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, ChinaCollege of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaNanjing Shineking Biotech Co., Ltd., Nanjing, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, ChinaCollege of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaJiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, ChinaCollege of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaJiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, ChinaCollege of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaJiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing, ChinaEndophytes have the potential to enhance the ability of plants to resist salt stress, improving crop development and yield. Therefore, in this study, we isolated an endophyte that produced large amounts of exopolysaccharides (EPSs) from the roots of sea rice and examined its effects on the physiological responses of rice (Oryza sativa L. ssp. japonica “Nipponbare”) seedlings to salt stress using hydroponic experiments. The endophyte was named Pantoea alhagi NX-11 based on its morphological characteristics and 16S ribosomal DNA (rDNA) sequence alignment. Rice seedlings that had been inoculated with P. alhagi NX-11 exhibited a 30.3% increase in fresh weight, a 28.6% increase in root length, a 51.6% increase in shoot length, and a 26.3% increase in chlorophyll content compared with control seedlings under normal conditions. In addition, inoculated rice seedlings had a 37.5% lower malondialdehyde content, a 133% higher K+/Na+ ratio, and a 52.8% higher proline content after 7 days under salt stress, as well as up-regulated expression of proline synthase, down-regulated expression of proline dehydrogenase, and enhanced antioxidant enzyme activities. Interestingly, rice seedlings that were inoculated with an EPS-deficient strain named NX-11eps– that was obtained by atmospheric and room temperature plasma (ARTP) mutagenesis were damaged by salt stress and had similar physiological and biochemical indicators to the control group. Therefore, we speculate that the ability of P. alhagi NX-11 to enhance the salt tolerance of rice seedlings is related to the EPSs it produces.https://www.frontiersin.org/article/10.3389/fmicb.2019.03112/fullendophytePantoea alhagiexopolysaccharidessalt stress ameliorationrice |
| spellingShingle | Liang Sun Liang Sun Liang Sun Peng Lei Peng Lei Peng Lei Qian Wang Qian Wang Junjie Ma Junjie Ma Yijing Zhan Yijing Zhan Yijing Zhan Kang Jiang Kang Jiang Kang Jiang Zongqi Xu Zongqi Xu Zongqi Xu Hong Xu Hong Xu Hong Xu The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting Exopolysaccharides Frontiers in Microbiology endophyte Pantoea alhagi exopolysaccharides salt stress amelioration rice |
| title | The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting Exopolysaccharides |
| title_full | The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting Exopolysaccharides |
| title_fullStr | The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting Exopolysaccharides |
| title_full_unstemmed | The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting Exopolysaccharides |
| title_short | The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting Exopolysaccharides |
| title_sort | endophyte pantoea alhagi nx 11 alleviates salt stress damage to rice seedlings by secreting exopolysaccharides |
| topic | endophyte Pantoea alhagi exopolysaccharides salt stress amelioration rice |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.03112/full |
| work_keys_str_mv | AT liangsun theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT liangsun theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT liangsun theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT penglei theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT penglei theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT penglei theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT qianwang theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT qianwang theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT junjiema theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT junjiema theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT yijingzhan theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT yijingzhan theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT yijingzhan theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT kangjiang theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT kangjiang theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT kangjiang theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT zongqixu theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT zongqixu theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT zongqixu theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT hongxu theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT hongxu theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT hongxu theendophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT liangsun endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT liangsun endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT liangsun endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT penglei endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT penglei endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT penglei endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT qianwang endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT qianwang endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT junjiema endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT junjiema endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT yijingzhan endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT yijingzhan endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT yijingzhan endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT kangjiang endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT kangjiang endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT kangjiang endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT zongqixu endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT zongqixu endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT zongqixu endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT hongxu endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT hongxu endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides AT hongxu endophytepantoeaalhaginx11alleviatessaltstressdamagetoriceseedlingsbysecretingexopolysaccharides |