A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle
Objective: To determine the polymorphism of GDF-9 gene in FH cattle that may affect its interaction with the BMP-15 protein. Method: Blood was taken from the jugular vein using 10 mL sterile syringes from ten Friesian Holstein cattle. The DNA were isolated from the whole blood and used as a template...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-04-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2305050016000191 |
| _version_ | 1818207265280229376 |
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| author | Afifi Inayah Sri Rahayu Nashi Widodo Widya Ayu Prasdini |
| author_facet | Afifi Inayah Sri Rahayu Nashi Widodo Widya Ayu Prasdini |
| author_sort | Afifi Inayah |
| collection | DOAJ |
| description | Objective: To determine the polymorphism of GDF-9 gene in FH cattle that may affect its interaction with the BMP-15 protein.
Method: Blood was taken from the jugular vein using 10 mL sterile syringes from ten Friesian Holstein cattle. The DNA were isolated from the whole blood and used as a template to amplify GDF-9 gene. The Amplicon (PCR product) was sequenced to identify the new SNP. The three-dimensional structure of GDF-9 protein was modeled by SWISS-MODEL. The characteristic of the protein structure was analyzed by using projectHOPE. The binding affinity GDF-9 into MBP was examined by PatchDock and FireDock.
Results: The result indicates a new variant G1358A changed amino acid residue at position 435 from Arginine into Histidine (R453H). In silico analysis using projectHOPE predicted that the variant altered side chain of GDF-9 and changed its interaction with BMP-15. Further study suggested that the polymorphism R453H also reduce the binding affinity of the GDF-9 into BMP-15 from −85.58 kcal/mol (R453) into −80.79 kcal/mol (H453).
Conclusion: The new variant G1358A at an estrous FH a cattle has potency alter GDF-9 profile that may affect to reproduction performance. |
| first_indexed | 2024-12-12T04:26:10Z |
| format | Article |
| id | doaj.art-cd5c9345cd6f4cb2976cf0810ef35e14 |
| institution | Directory Open Access Journal |
| issn | 2305-0500 |
| language | English |
| last_indexed | 2024-12-12T04:26:10Z |
| publishDate | 2016-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-cd5c9345cd6f4cb2976cf0810ef35e142022-12-22T00:38:11ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002016-04-015214014310.1016/j.apjr.2016.01.010A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattleAfifi Inayah0Sri Rahayu1Nashi Widodo2Widya Ayu Prasdini3Biology Department, Mathematics and Science Faculty, Brawijaya University, Malang, East Java, IndonesiaBiology Department, Mathematics and Science Faculty, Brawijaya University, Malang, East Java, IndonesiaBiology Department, Mathematics and Science Faculty, Brawijaya University, Malang, East Java, IndonesiaTraining Center for Animal Husbandry, Batu, East Java, IndonesiaObjective: To determine the polymorphism of GDF-9 gene in FH cattle that may affect its interaction with the BMP-15 protein. Method: Blood was taken from the jugular vein using 10 mL sterile syringes from ten Friesian Holstein cattle. The DNA were isolated from the whole blood and used as a template to amplify GDF-9 gene. The Amplicon (PCR product) was sequenced to identify the new SNP. The three-dimensional structure of GDF-9 protein was modeled by SWISS-MODEL. The characteristic of the protein structure was analyzed by using projectHOPE. The binding affinity GDF-9 into MBP was examined by PatchDock and FireDock. Results: The result indicates a new variant G1358A changed amino acid residue at position 435 from Arginine into Histidine (R453H). In silico analysis using projectHOPE predicted that the variant altered side chain of GDF-9 and changed its interaction with BMP-15. Further study suggested that the polymorphism R453H also reduce the binding affinity of the GDF-9 into BMP-15 from −85.58 kcal/mol (R453) into −80.79 kcal/mol (H453). Conclusion: The new variant G1358A at an estrous FH a cattle has potency alter GDF-9 profile that may affect to reproduction performance.http://www.sciencedirect.com/science/article/pii/S2305050016000191BMP-15FolliculogenesisGDF-9In silicoDocking molecule |
| spellingShingle | Afifi Inayah Sri Rahayu Nashi Widodo Widya Ayu Prasdini A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle Asian Pacific Journal of Reproduction BMP-15 Folliculogenesis GDF-9 In silico Docking molecule |
| title | A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle |
| title_full | A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle |
| title_fullStr | A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle |
| title_full_unstemmed | A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle |
| title_short | A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle |
| title_sort | new nucleotide variant g1358a potentially change growth differentiation factor 9 profile that may affect the reproduction performance of friesian holstein cattle |
| topic | BMP-15 Folliculogenesis GDF-9 In silico Docking molecule |
| url | http://www.sciencedirect.com/science/article/pii/S2305050016000191 |
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