Optimization of the culture medium and fermentation conditions for Pseudomonas fluorescens-based biofertilizers production
In Cuba, a biofertilizer has been developed based on a Pseudomonas fluorescens strain phosphate solubilizer. Given the need to standardize its industrial scale up, this work aimed to optimize the culture medium and the fermentation conditions. First, tests in the shaker were carried out and then in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2019-06-01
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| Series: | Biotecnología Vegetal |
| Subjects: | |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/624 |
| _version_ | 1811271440889020416 |
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| author | María Cristina Pérez Peñaranda Julio Oramas García Emilio Arcadio Sotolongo Valdés Alina Miranda Galuzzo Yoandra Román Tabio Alain González Soto |
| author_facet | María Cristina Pérez Peñaranda Julio Oramas García Emilio Arcadio Sotolongo Valdés Alina Miranda Galuzzo Yoandra Román Tabio Alain González Soto |
| author_sort | María Cristina Pérez Peñaranda |
| collection | DOAJ |
| description | In Cuba, a biofertilizer has been developed based on a Pseudomonas fluorescens strain phosphate solubilizer. Given the need to standardize its industrial scale up, this work aimed to optimize the culture medium and the fermentation conditions. First, tests in the shaker were carried out and then in a fermenter. In each case an experimental validation was performed. For the optimization of the culture medium, a central composite design was used to adjust response surfaces. A 23 factorial design with equidistant star points and six repetitions in the center of the plan considered zero was applied. A total of 20 experimental runs were performed, with three repetitions each and the regression equation was determined. As a response variable, cell growth was determined by optical density λ = 600 nm (OD600). To optimize microbial growth based on agitation speed and air flow, a 12-liter total volume fermenter with 8.5 liters of effective volume with a factorial design 22 was used, taking OD600 as the response variable. It was found that for the industrial production of P. fluorescens and the formulation of the bioproduct, an optimized semisynthetic culture medium can be used, without molasses. Together with the employed of optimal agitation speed and air flow, the bacterial biomass content can be increased. The new conditions allowed reducing the fermentation time to 12 hours compared to the 20 to 22 hours used in the production scheme. In addition, 29%, 50% and 40%, respectively, of carbon, nitrogen and phosphorus sources were reduced. |
| first_indexed | 2024-04-12T22:21:06Z |
| format | Article |
| id | doaj.art-cd60d9399fe04ed882d43e0b2902f855 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-04-12T22:21:06Z |
| publishDate | 2019-06-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-cd60d9399fe04ed882d43e0b2902f8552022-12-22T03:14:22ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472019-06-01192127138Optimization of the culture medium and fermentation conditions for Pseudomonas fluorescens-based biofertilizers productionMaría Cristina Pérez Peñaranda0Julio Oramas García1 Emilio Arcadio Sotolongo Valdés2Alina Miranda Galuzzo3Yoandra Román Tabio4Alain González Soto5 Unidad de Desarrollo–Innovación del Grupo Empresarial Laboratorios Biológicos Farmacéuticos (LABIOFAM). Ave. Independencia km 16 ½. Boyeros. La Habana. Cuba.; Cuba Unidad de Desarrollo–Innovación del Grupo Empresarial Laboratorios Biológicos Farmacéuticos (LABIOFAM). Ave. Independencia km 16 ½. Boyeros. La Habana. Cuba.; Cuba Facultad de Ingeniería Química, Universidad Tecnológica de La Habana. Calle 114, No. 11901, entre Ciclovía y Rotonda. Marianao. La Habana. Cuba.; Cuba Unidad Empresarial de Base VIMANG-LABIOFAM. Arroyo Naranjo. La Habana. Cuba.; Cuba Unidad de Desarrollo–Innovación del Grupo Empresarial Laboratorios Biológicos Farmacéuticos (LABIOFAM). Ave. Independencia km 16 ½. Boyeros. La Habana. Cuba.; Cuba Facultad de Ingeniería Química, Universidad Tecnológica de La Habana. Calle 114, No. 11901, entre Ciclovía y Rotonda. Marianao. La Habana. Cuba.; Cuba In Cuba, a biofertilizer has been developed based on a Pseudomonas fluorescens strain phosphate solubilizer. Given the need to standardize its industrial scale up, this work aimed to optimize the culture medium and the fermentation conditions. First, tests in the shaker were carried out and then in a fermenter. In each case an experimental validation was performed. For the optimization of the culture medium, a central composite design was used to adjust response surfaces. A 23 factorial design with equidistant star points and six repetitions in the center of the plan considered zero was applied. A total of 20 experimental runs were performed, with three repetitions each and the regression equation was determined. As a response variable, cell growth was determined by optical density λ = 600 nm (OD600). To optimize microbial growth based on agitation speed and air flow, a 12-liter total volume fermenter with 8.5 liters of effective volume with a factorial design 22 was used, taking OD600 as the response variable. It was found that for the industrial production of P. fluorescens and the formulation of the bioproduct, an optimized semisynthetic culture medium can be used, without molasses. Together with the employed of optimal agitation speed and air flow, the bacterial biomass content can be increased. The new conditions allowed reducing the fermentation time to 12 hours compared to the 20 to 22 hours used in the production scheme. In addition, 29%, 50% and 40%, respectively, of carbon, nitrogen and phosphorus sources were reduced.https://revista.ibp.co.cu/index.php/BV/article/view/624bioproductcentral composite designresponse surface methodology |
| spellingShingle | María Cristina Pérez Peñaranda Julio Oramas García Emilio Arcadio Sotolongo Valdés Alina Miranda Galuzzo Yoandra Román Tabio Alain González Soto Optimization of the culture medium and fermentation conditions for Pseudomonas fluorescens-based biofertilizers production Biotecnología Vegetal bioproduct central composite design response surface methodology |
| title | Optimization of the culture medium and fermentation conditions for Pseudomonas fluorescens-based biofertilizers production |
| title_full | Optimization of the culture medium and fermentation conditions for Pseudomonas fluorescens-based biofertilizers production |
| title_fullStr | Optimization of the culture medium and fermentation conditions for Pseudomonas fluorescens-based biofertilizers production |
| title_full_unstemmed | Optimization of the culture medium and fermentation conditions for Pseudomonas fluorescens-based biofertilizers production |
| title_short | Optimization of the culture medium and fermentation conditions for Pseudomonas fluorescens-based biofertilizers production |
| title_sort | optimization of the culture medium and fermentation conditions for pseudomonas fluorescens based biofertilizers production |
| topic | bioproduct central composite design response surface methodology |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/624 |
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